Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies

Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (...
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Gespeichert in:

Autor*in:	Xuan Zhu [verfasserIn] Jing Wang [verfasserIn] Jia Lv [verfasserIn] Pingping Liu [verfasserIn] Lingling Zhang [verfasserIn] Wenqian Jiao [verfasserIn] Cen Ma [verfasserIn] Zhenmin Bao [verfasserIn] Shi Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Übergeordnetes Werk:	In: Evolutionary Bioinformatics - SAGE Publishing, 2007, 15(2019)
Übergeordnetes Werk:	volume:15 ; year:2019

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1177/1176934319836074

Katalog-ID:	DOAJ036190780

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allfieldsSound	10.1177/1176934319836074 doi (DE-627)DOAJ036190780 (DE-599)DOAJfeef4dd39b25486680a9c411b045ba62 DE-627 ger DE-627 rakwb eng QH359-425 Xuan Zhu verfasserin aut Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies. Evolution Jing Wang verfasserin aut Jia Lv verfasserin aut Pingping Liu verfasserin aut Lingling Zhang verfasserin aut Wenqian Jiao verfasserin aut Cen Ma verfasserin aut Zhenmin Bao verfasserin aut Shi Wang verfasserin aut In Evolutionary Bioinformatics SAGE Publishing, 2007 15(2019) (DE-627)509754260 (DE-600)2227610-5 11769343 nnns volume:15 year:2019 https://doi.org/10.1177/1176934319836074 kostenfrei https://doaj.org/article/feef4dd39b25486680a9c411b045ba62 kostenfrei https://doi.org/10.1177/1176934319836074 kostenfrei https://doaj.org/toc/1176-9343 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019
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author	Xuan Zhu
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topic_title	QH359-425 Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies
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title	Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies
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title_sort	sequencing-based transcriptome-wide targeted genotyping for evolutionary and ecological studies
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title_auth	Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies
abstract	Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies.
abstractGer	Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies.
abstract_unstemmed	Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies.
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title_short	Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies
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Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies

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