FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]

Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine h...
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Autor*in:	Mita Gebriella Inthe [verfasserIn] Kustiariyah Tarman* [verfasserIn] Mega Safithri [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Indonesisch

Erschienen:	2014

Schlagwörter:	BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii

Übergeordnetes Werk:	In: Jurnal Teknologi dan Industri Pangan - Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013, 25(2014), 1, Seite 39-46
Übergeordnetes Werk:	volume:25 ; year:2014 ; number:1 ; pages:39-46

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.6066/jtip.2014.25.1.39

Katalog-ID:	DOAJ036391670

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520			\|a Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
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allfields	10.6066/jtip.2014.25.1.39 doi (DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa DE-627 ger DE-627 rakwb eng ind TP368-456 Mita Gebriella Inthe verfasserin aut FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells. BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture Kustiariyah Tarman* verfasserin aut Mega Safithri verfasserin aut In Jurnal Teknologi dan Industri Pangan Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013 25(2014), 1, Seite 39-46 (DE-627)1760592196 2087751X nnns volume:25 year:2014 number:1 pages:39-46 https://doi.org/10.6066/jtip.2014.25.1.39 kostenfrei https://doaj.org/article/6d9b31b70766457db72e57cd3b1ff6aa kostenfrei http://journal.ipb.ac.id/index.php/jtip/article/view/8301 kostenfrei https://doaj.org/toc/1979-7788 Journal toc kostenfrei https://doaj.org/toc/2087-751X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_206 GBV_ILN_224 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2014 1 39-46
spelling	10.6066/jtip.2014.25.1.39 doi (DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa DE-627 ger DE-627 rakwb eng ind TP368-456 Mita Gebriella Inthe verfasserin aut FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells. BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture Kustiariyah Tarman* verfasserin aut Mega Safithri verfasserin aut In Jurnal Teknologi dan Industri Pangan Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013 25(2014), 1, Seite 39-46 (DE-627)1760592196 2087751X nnns volume:25 year:2014 number:1 pages:39-46 https://doi.org/10.6066/jtip.2014.25.1.39 kostenfrei https://doaj.org/article/6d9b31b70766457db72e57cd3b1ff6aa kostenfrei http://journal.ipb.ac.id/index.php/jtip/article/view/8301 kostenfrei https://doaj.org/toc/1979-7788 Journal toc kostenfrei https://doaj.org/toc/2087-751X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_206 GBV_ILN_224 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2014 1 39-46
allfields_unstemmed	10.6066/jtip.2014.25.1.39 doi (DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa DE-627 ger DE-627 rakwb eng ind TP368-456 Mita Gebriella Inthe verfasserin aut FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells. BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture Kustiariyah Tarman* verfasserin aut Mega Safithri verfasserin aut In Jurnal Teknologi dan Industri Pangan Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013 25(2014), 1, Seite 39-46 (DE-627)1760592196 2087751X nnns volume:25 year:2014 number:1 pages:39-46 https://doi.org/10.6066/jtip.2014.25.1.39 kostenfrei https://doaj.org/article/6d9b31b70766457db72e57cd3b1ff6aa kostenfrei http://journal.ipb.ac.id/index.php/jtip/article/view/8301 kostenfrei https://doaj.org/toc/1979-7788 Journal toc kostenfrei https://doaj.org/toc/2087-751X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_206 GBV_ILN_224 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2014 1 39-46
allfieldsGer	10.6066/jtip.2014.25.1.39 doi (DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa DE-627 ger DE-627 rakwb eng ind TP368-456 Mita Gebriella Inthe verfasserin aut FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells. BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture Kustiariyah Tarman* verfasserin aut Mega Safithri verfasserin aut In Jurnal Teknologi dan Industri Pangan Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013 25(2014), 1, Seite 39-46 (DE-627)1760592196 2087751X nnns volume:25 year:2014 number:1 pages:39-46 https://doi.org/10.6066/jtip.2014.25.1.39 kostenfrei https://doaj.org/article/6d9b31b70766457db72e57cd3b1ff6aa kostenfrei http://journal.ipb.ac.id/index.php/jtip/article/view/8301 kostenfrei https://doaj.org/toc/1979-7788 Journal toc kostenfrei https://doaj.org/toc/2087-751X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_206 GBV_ILN_224 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2014 1 39-46
allfieldsSound	10.6066/jtip.2014.25.1.39 doi (DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa DE-627 ger DE-627 rakwb eng ind TP368-456 Mita Gebriella Inthe verfasserin aut FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells. BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture Kustiariyah Tarman* verfasserin aut Mega Safithri verfasserin aut In Jurnal Teknologi dan Industri Pangan Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013 25(2014), 1, Seite 39-46 (DE-627)1760592196 2087751X nnns volume:25 year:2014 number:1 pages:39-46 https://doi.org/10.6066/jtip.2014.25.1.39 kostenfrei https://doaj.org/article/6d9b31b70766457db72e57cd3b1ff6aa kostenfrei http://journal.ipb.ac.id/index.php/jtip/article/view/8301 kostenfrei https://doaj.org/toc/1979-7788 Journal toc kostenfrei https://doaj.org/toc/2087-751X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_206 GBV_ILN_224 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2014 1 39-46
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source	In Jurnal Teknologi dan Industri Pangan 25(2014), 1, Seite 39-46 volume:25 year:2014 number:1 pages:39-46
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topic_facet	BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii Food processing and manufacture
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One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. 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callnumber-first	T - Technology
author	Mita Gebriella Inthe
spellingShingle	Mita Gebriella Inthe misc TP368-456 misc BSLT misc cervical cancer misc fractionation misc Marine fungus misc MTT assay misc Xylaria psidii misc Food processing and manufacture FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]
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topic_title	TP368-456 FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] BSLT cervical cancer fractionation Marine fungus MTT assay Xylaria psidii
topic	misc TP368-456 misc BSLT misc cervical cancer misc fractionation misc Marine fungus misc MTT assay misc Xylaria psidii misc Food processing and manufacture
topic_unstemmed	misc TP368-456 misc BSLT misc cervical cancer misc fractionation misc Marine fungus misc MTT assay misc Xylaria psidii misc Food processing and manufacture
topic_browse	misc TP368-456 misc BSLT misc cervical cancer misc fractionation misc Marine fungus misc MTT assay misc Xylaria psidii misc Food processing and manufacture
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title	FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]
ctrlnum	(DE-627)DOAJ036391670 (DE-599)DOAJ6d9b31b70766457db72e57cd3b1ff6aa
title_full	FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]
author_sort	Mita Gebriella Inthe
journal	Jurnal Teknologi dan Industri Pangan
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author_browse	Mita Gebriella Inthe Kustiariyah Tarman* Mega Safithri
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class	TP368-456
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author-letter	Mita Gebriella Inthe
doi_str_mv	10.6066/jtip.2014.25.1.39
author2-role	verfasserin
title_sort	fraksinasi protein kapang laut xylaria psidii kt30 dan sitotoksisitasnya terhadap sel hela [fractionation of proteins of marine fungus xylaria psidii kt30 and their cytotoxicity against hela cells]
callnumber	TP368-456
title_auth	FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]
abstract	Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
abstractGer	Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
abstract_unstemmed	Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
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title_short	FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]
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up_date	2024-07-03T20:19:44.538Z
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