Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques

Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Middelveen MJ [verfasserIn] Du Cruz I [verfasserIn] Fesler MC [verfasserIn] Stricker RB [verfasserIn] Shah JS [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease.

Übergeordnetes Werk:	In: Clinical, Cosmetic and Investigational Dermatology - Dove Medical Press, 2009, (2018), Seite 561-569
Übergeordnetes Werk:	year:2018 ; pages:561-569

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ036489468

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520			\|a Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease
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allfields	(DE-627)DOAJ036489468 (DE-599)DOAJ51ec54bba21e499086b9830c5221d5f2 DE-627 ger DE-627 rakwb eng RL1-803 Middelveen MJ verfasserin aut Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology Du Cruz I verfasserin aut Fesler MC verfasserin aut Stricker RB verfasserin aut Shah JS verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2018), Seite 561-569 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2018 pages:561-569 https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 kostenfrei https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 561-569
spelling	(DE-627)DOAJ036489468 (DE-599)DOAJ51ec54bba21e499086b9830c5221d5f2 DE-627 ger DE-627 rakwb eng RL1-803 Middelveen MJ verfasserin aut Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology Du Cruz I verfasserin aut Fesler MC verfasserin aut Stricker RB verfasserin aut Shah JS verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2018), Seite 561-569 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2018 pages:561-569 https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 kostenfrei https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 561-569
allfields_unstemmed	(DE-627)DOAJ036489468 (DE-599)DOAJ51ec54bba21e499086b9830c5221d5f2 DE-627 ger DE-627 rakwb eng RL1-803 Middelveen MJ verfasserin aut Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology Du Cruz I verfasserin aut Fesler MC verfasserin aut Stricker RB verfasserin aut Shah JS verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2018), Seite 561-569 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2018 pages:561-569 https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 kostenfrei https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 561-569
allfieldsGer	(DE-627)DOAJ036489468 (DE-599)DOAJ51ec54bba21e499086b9830c5221d5f2 DE-627 ger DE-627 rakwb eng RL1-803 Middelveen MJ verfasserin aut Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology Du Cruz I verfasserin aut Fesler MC verfasserin aut Stricker RB verfasserin aut Shah JS verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2018), Seite 561-569 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2018 pages:561-569 https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 kostenfrei https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 561-569
allfieldsSound	(DE-627)DOAJ036489468 (DE-599)DOAJ51ec54bba21e499086b9830c5221d5f2 DE-627 ger DE-627 rakwb eng RL1-803 Middelveen MJ verfasserin aut Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology Du Cruz I verfasserin aut Fesler MC verfasserin aut Stricker RB verfasserin aut Shah JS verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2018), Seite 561-569 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2018 pages:561-569 https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 kostenfrei https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 561-569
language	English
source	In Clinical, Cosmetic and Investigational Dermatology (2018), Seite 561-569 year:2018 pages:561-569
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topic_facet	Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease. Dermatology
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author	Middelveen MJ
spellingShingle	Middelveen MJ misc RL1-803 misc Lyme disease misc Borrelia burgdorferi misc Morgellons disease misc Relapsing fever Borrelia misc Bartonella misc Tickborne disease. misc Dermatology Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
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topic_title	RL1-803 Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques Lyme disease Borrelia burgdorferi Morgellons disease Relapsing fever Borrelia Bartonella Tickborne disease
topic	misc RL1-803 misc Lyme disease misc Borrelia burgdorferi misc Morgellons disease misc Relapsing fever Borrelia misc Bartonella misc Tickborne disease. misc Dermatology
topic_unstemmed	misc RL1-803 misc Lyme disease misc Borrelia burgdorferi misc Morgellons disease misc Relapsing fever Borrelia misc Bartonella misc Tickborne disease. misc Dermatology
topic_browse	misc RL1-803 misc Lyme disease misc Borrelia burgdorferi misc Morgellons disease misc Relapsing fever Borrelia misc Bartonella misc Tickborne disease. misc Dermatology
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title	Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
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title_full	Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
author_sort	Middelveen MJ
journal	Clinical, Cosmetic and Investigational Dermatology
journalStr	Clinical, Cosmetic and Investigational Dermatology
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author_browse	Middelveen MJ Du Cruz I Fesler MC Stricker RB Shah JS
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author-letter	Middelveen MJ
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title_sort	detection of tick-borne infection in morgellons disease patients by serological and molecular techniques
callnumber	RL1-803
title_auth	Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
abstract	Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease
abstractGer	Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease
abstract_unstemmed	Marianne J Middelveen,1 Iris Du Cruz,2 Melissa C Fesler,3 Raphael B Stricker,3 Jyotsna S Shah2 1Atkins Veterinary Services, Calgary, AB, Canada; 2IGeneX Laboratories, Palo Alto, CA, USA; 3Union Square Medical Associates, San Francisco, CA, USA Background: Morgellons disease (MD) is a skin condition associated with Lyme disease (LD) and tick-borne illness. Patients with this skin disorder experience ulcerative lesions that contain multicolored filamentous collagen and keratin inclusions. Infection with various species of Borrelia and other tick-borne pathogens has been detected in tissue and body fluid specimens from MD patients. We sought to explore this association further in a cohort of MD patients.Patients and methods: Sera from 30 patients with MD were tested for antibody reactivity to antigens from the Borrelia burgdorferi (Bb) group and the relapsing fever Borrelia (RFB) group of spirochetes. Tissue and/or body fluid specimens from these patients were also tested for the presence of Bb and RFB infection using PCR technology. In addition, tissue and body fluid specimens were tested for the presence of Bartonella henselae using PCR, and formalin-fixed skin sections from a subset of patients were tested using fluorescent in situ hybridization (FISH) with B. henselae-specific DNA probes.Results: Seroreactivity to Bb, RFB or both was detected in 63% of the cohort, while positive PCR testing for Bb, RFB or both was detected in 53% of the cohort. Overall, 90% of patients tested positive for exposure and/or infection with Borrelia spirochetes. B. henselae infection was detected by PCR in skin sections or body fluids from 20% of the subjects, and B. henselae FISH testing was positive in 30% of the dermatological specimens submitted for study.Conclusion: The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness. Keywords: Lyme disease, Borrelia burgdorferi, Morgellons disease, relapsing fever Borrelia, Bartonella, tick-borne disease
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title_short	Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
url	https://doaj.org/article/51ec54bba21e499086b9830c5221d5f2 https://www.dovepress.com/detection-of-tick-borne-infection-in-morgellons-disease-patients-by-se-peer-reviewed-article-CCID https://doaj.org/toc/1178-7015
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