Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

<p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Bastien Thomas [verfasserIn] van den Broeck Hetty C [verfasserIn] van der Meer Ingrid M [verfasserIn] Bas Noor [verfasserIn] Goryunova Svetlana V [verfasserIn] Salentijn Elma MJ [verfasserIn] Gilissen Luud JWJ [verfasserIn] Smulders Marinus JM [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 10(2009), 1, p 48
Übergeordnetes Werk:	volume:10 ; year:2009 ; number:1, p 48

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-10-48

Katalog-ID:	DOAJ036638153

Internformat


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245	1	0	\|a Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
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700	0		\|a Smulders Marinus JM \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
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author_variant	b t bt v d b h c vdbhc v d m i m vdmim b n bn g s v gsv s e m sem g l j glj s m j smj
matchkey_str	article:14712164:2009----::erpodnhxpodhavreiseelagdfeecsnxrsinflhgi
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allfields	10.1186/1471-2164-10-48 doi (DE-627)DOAJ036638153 (DE-599)DOAJc1d03ecf88f34536be4435675308f1fe DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Bastien Thomas verfasserin aut Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Biotechnology Genetics van den Broeck Hetty C verfasserin aut van der Meer Ingrid M verfasserin aut Bas Noor verfasserin aut Goryunova Svetlana V verfasserin aut Salentijn Elma MJ verfasserin aut Gilissen Luud JWJ verfasserin aut Smulders Marinus JM verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 48 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 48 https://doi.org/10.1186/1471-2164-10-48 kostenfrei https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe kostenfrei http://www.biomedcentral.com/1471-2164/10/48 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 48
spelling	10.1186/1471-2164-10-48 doi (DE-627)DOAJ036638153 (DE-599)DOAJc1d03ecf88f34536be4435675308f1fe DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Bastien Thomas verfasserin aut Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Biotechnology Genetics van den Broeck Hetty C verfasserin aut van der Meer Ingrid M verfasserin aut Bas Noor verfasserin aut Goryunova Svetlana V verfasserin aut Salentijn Elma MJ verfasserin aut Gilissen Luud JWJ verfasserin aut Smulders Marinus JM verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 48 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 48 https://doi.org/10.1186/1471-2164-10-48 kostenfrei https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe kostenfrei http://www.biomedcentral.com/1471-2164/10/48 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 48
allfields_unstemmed	10.1186/1471-2164-10-48 doi (DE-627)DOAJ036638153 (DE-599)DOAJc1d03ecf88f34536be4435675308f1fe DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Bastien Thomas verfasserin aut Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Biotechnology Genetics van den Broeck Hetty C verfasserin aut van der Meer Ingrid M verfasserin aut Bas Noor verfasserin aut Goryunova Svetlana V verfasserin aut Salentijn Elma MJ verfasserin aut Gilissen Luud JWJ verfasserin aut Smulders Marinus JM verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 48 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 48 https://doi.org/10.1186/1471-2164-10-48 kostenfrei https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe kostenfrei http://www.biomedcentral.com/1471-2164/10/48 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 48
allfieldsGer	10.1186/1471-2164-10-48 doi (DE-627)DOAJ036638153 (DE-599)DOAJc1d03ecf88f34536be4435675308f1fe DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Bastien Thomas verfasserin aut Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Biotechnology Genetics van den Broeck Hetty C verfasserin aut van der Meer Ingrid M verfasserin aut Bas Noor verfasserin aut Goryunova Svetlana V verfasserin aut Salentijn Elma MJ verfasserin aut Gilissen Luud JWJ verfasserin aut Smulders Marinus JM verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 48 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 48 https://doi.org/10.1186/1471-2164-10-48 kostenfrei https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe kostenfrei http://www.biomedcentral.com/1471-2164/10/48 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 48
allfieldsSound	10.1186/1471-2164-10-48 doi (DE-627)DOAJ036638153 (DE-599)DOAJc1d03ecf88f34536be4435675308f1fe DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Bastien Thomas verfasserin aut Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p< Biotechnology Genetics van den Broeck Hetty C verfasserin aut van der Meer Ingrid M verfasserin aut Bas Noor verfasserin aut Goryunova Svetlana V verfasserin aut Salentijn Elma MJ verfasserin aut Gilissen Luud JWJ verfasserin aut Smulders Marinus JM verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 48 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 48 https://doi.org/10.1186/1471-2164-10-48 kostenfrei https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe kostenfrei http://www.biomedcentral.com/1471-2164/10/48 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 48
language	English
source	In BMC Genomics 10(2009), 1, p 48 volume:10 year:2009 number:1, p 48
sourceStr	In BMC Genomics 10(2009), 1, p 48 volume:10 year:2009 number:1, p 48
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Bastien Thomas @@aut@@ van den Broeck Hetty C @@aut@@ van der Meer Ingrid M @@aut@@ Bas Noor @@aut@@ Goryunova Svetlana V @@aut@@ Salentijn Elma MJ @@aut@@ Gilissen Luud JWJ @@aut@@ Smulders Marinus JM @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ036638153
language_de	englisch
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These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. 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callnumber-first	T - Technology
author	Bastien Thomas
spellingShingle	Bastien Thomas misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
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topic_title	TP248.13-248.65 QH426-470 Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
topic	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
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title	Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
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title_full	Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
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author_browse	Bastien Thomas van den Broeck Hetty C van der Meer Ingrid M Bas Noor Goryunova Svetlana V Salentijn Elma MJ Gilissen Luud JWJ Smulders Marinus JM
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title_sort	tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous gli-2 loci
callnumber	TP248.13-248.65
title_auth	Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
abstract	<p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Α-gliadins form a multigene protein family encoded by multiple α-gliadin (<it<Gli-2</it<) genes at three genomic loci, <it<Gli-A2</it<, <it<Gli-B2 </it<and <it<Gli-D2</it<, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.</p<
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title_short	Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci
url	https://doi.org/10.1186/1471-2164-10-48 https://doaj.org/article/c1d03ecf88f34536be4435675308f1fe http://www.biomedcentral.com/1471-2164/10/48 https://doaj.org/toc/1471-2164
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up_date	2024-07-03T21:42:11.331Z
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These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the <it<Gli-B2 </it<locus harbour fewer conserved CD-epitopes than those from <it<Gli-A2</it<, whereas the <it<Gli-D2 </it<gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous <it<Gli-2 </it<loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.</p< <p<Results</p< <p<We detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for <it<Gli-A2 </it<genes. The relative <it<Gli-A2 </it<expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the <it<Gli-A2 </it<frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus <it<Gli-B2 </it<were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').</p< <p<Conclusion</p< <p<Here, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous <it<Gli-2 </it<loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous <it<Gli-2 </it<loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. 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Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

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