Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress

Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Alberto Marco Del Pino [verfasserIn] Luca Regni [verfasserIn] Roberto D’Amato [verfasserIn] Emma Tedeschini [verfasserIn] Daniela Businelli [verfasserIn] Primo Proietti [verfasserIn] Carlo Alberto Palmerini [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	olive se-fertilization selenium se-methionine cytosolic Ca2+ pollen germination

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 10(2019)
Übergeordnetes Werk:	volume:10 ; year:2019

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2019.01611

Katalog-ID:	DOAJ036858358

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520			\|a Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity.
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allfieldsGer	10.3389/fpls.2019.01611 doi (DE-627)DOAJ036858358 (DE-599)DOAJd1ba4c7950da4c34b740734a72ebcea1 DE-627 ger DE-627 rakwb eng SB1-1110 Alberto Marco Del Pino verfasserin aut Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity. olive se-fertilization selenium se-methionine cytosolic Ca2+ pollen germination Plant culture Luca Regni verfasserin aut Roberto D’Amato verfasserin aut Emma Tedeschini verfasserin aut Daniela Businelli verfasserin aut Primo Proietti verfasserin aut Carlo Alberto Palmerini verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 10(2019) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:10 year:2019 https://doi.org/10.3389/fpls.2019.01611 kostenfrei https://doaj.org/article/d1ba4c7950da4c34b740734a72ebcea1 kostenfrei https://www.frontiersin.org/article/10.3389/fpls.2019.01611/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
allfieldsSound	10.3389/fpls.2019.01611 doi (DE-627)DOAJ036858358 (DE-599)DOAJd1ba4c7950da4c34b740734a72ebcea1 DE-627 ger DE-627 rakwb eng SB1-1110 Alberto Marco Del Pino verfasserin aut Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity. olive se-fertilization selenium se-methionine cytosolic Ca2+ pollen germination Plant culture Luca Regni verfasserin aut Roberto D’Amato verfasserin aut Emma Tedeschini verfasserin aut Daniela Businelli verfasserin aut Primo Proietti verfasserin aut Carlo Alberto Palmerini verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 10(2019) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:10 year:2019 https://doi.org/10.3389/fpls.2019.01611 kostenfrei https://doaj.org/article/d1ba4c7950da4c34b740734a72ebcea1 kostenfrei https://www.frontiersin.org/article/10.3389/fpls.2019.01611/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
language	English
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topic_facet	olive se-fertilization selenium se-methionine cytosolic Ca2+ pollen germination Plant culture
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container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Alberto Marco Del Pino @@aut@@ Luca Regni @@aut@@ Roberto D’Amato @@aut@@ Emma Tedeschini @@aut@@ Daniela Businelli @@aut@@ Primo Proietti @@aut@@ Carlo Alberto Palmerini @@aut@@
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callnumber-first	S - Agriculture
author	Alberto Marco Del Pino
spellingShingle	Alberto Marco Del Pino misc SB1-1110 misc olive misc se-fertilization misc selenium misc se-methionine misc cytosolic Ca2+ misc pollen germination misc Plant culture Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress
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topic_title	SB1-1110 Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress olive se-fertilization selenium se-methionine cytosolic Ca2+ pollen germination
topic	misc SB1-1110 misc olive misc se-fertilization misc selenium misc se-methionine misc cytosolic Ca2+ misc pollen germination misc Plant culture
topic_unstemmed	misc SB1-1110 misc olive misc se-fertilization misc selenium misc se-methionine misc cytosolic Ca2+ misc pollen germination misc Plant culture
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title	Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress
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title_full	Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress
author_sort	Alberto Marco Del Pino
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author_browse	Alberto Marco Del Pino Luca Regni Roberto D’Amato Emma Tedeschini Daniela Businelli Primo Proietti Carlo Alberto Palmerini
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title_sort	selenium-enriched pollen grains of olea europaea l.: ca2+ signaling and germination under oxidative stress
callnumber	SB1-1110
title_auth	Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress
abstract	Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity.
abstractGer	Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity.
abstract_unstemmed	Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H2O2 to produce an oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 interrupted Ca2+ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca2+ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H2O2. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca2+ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress
url	https://doi.org/10.3389/fpls.2019.01611 https://doaj.org/article/d1ba4c7950da4c34b740734a72ebcea1 https://www.frontiersin.org/article/10.3389/fpls.2019.01611/full https://doaj.org/toc/1664-462X
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up_date	2024-07-03T22:50:36.553Z
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Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress

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