Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI
Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* map...
Ausführliche Beschreibung
Autor*in: |
Johanna Buschmann [verfasserIn] Dorothea M. Heuberger [verfasserIn] Fatma Kivrak Pfiffner [verfasserIn] Petra Wolint [verfasserIn] Jae-Hwi Jang [verfasserIn] Wolfgang Jungraithmayr [verfasserIn] Pietro Giovanoli [verfasserIn] Maurizio Calcagni [verfasserIn] Conny F. Waschkies [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts |
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Übergeordnetes Werk: |
In: Cancers - MDPI AG, 2010, 14(2022), 13, p 3114 |
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Übergeordnetes Werk: |
volume:14 ; year:2022 ; number:13, p 3114 |
Links: |
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DOI / URN: |
10.3390/cancers14133114 |
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Katalog-ID: |
DOAJ036875015 |
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520 | |a Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. | ||
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10.3390/cancers14133114 doi (DE-627)DOAJ036875015 (DE-599)DOAJeb4f2f99aead47448998e63899b4cc5b DE-627 ger DE-627 rakwb eng RC254-282 Johanna Buschmann verfasserin aut Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Dorothea M. Heuberger verfasserin aut Fatma Kivrak Pfiffner verfasserin aut Petra Wolint verfasserin aut Jae-Hwi Jang verfasserin aut Wolfgang Jungraithmayr verfasserin aut Pietro Giovanoli verfasserin aut Maurizio Calcagni verfasserin aut Conny F. Waschkies verfasserin aut In Cancers MDPI AG, 2010 14(2022), 13, p 3114 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:14 year:2022 number:13, p 3114 https://doi.org/10.3390/cancers14133114 kostenfrei https://doaj.org/article/eb4f2f99aead47448998e63899b4cc5b kostenfrei https://www.mdpi.com/2072-6694/14/13/3114 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 13, p 3114 |
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10.3390/cancers14133114 doi (DE-627)DOAJ036875015 (DE-599)DOAJeb4f2f99aead47448998e63899b4cc5b DE-627 ger DE-627 rakwb eng RC254-282 Johanna Buschmann verfasserin aut Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Dorothea M. Heuberger verfasserin aut Fatma Kivrak Pfiffner verfasserin aut Petra Wolint verfasserin aut Jae-Hwi Jang verfasserin aut Wolfgang Jungraithmayr verfasserin aut Pietro Giovanoli verfasserin aut Maurizio Calcagni verfasserin aut Conny F. Waschkies verfasserin aut In Cancers MDPI AG, 2010 14(2022), 13, p 3114 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:14 year:2022 number:13, p 3114 https://doi.org/10.3390/cancers14133114 kostenfrei https://doaj.org/article/eb4f2f99aead47448998e63899b4cc5b kostenfrei https://www.mdpi.com/2072-6694/14/13/3114 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 13, p 3114 |
allfields_unstemmed |
10.3390/cancers14133114 doi (DE-627)DOAJ036875015 (DE-599)DOAJeb4f2f99aead47448998e63899b4cc5b DE-627 ger DE-627 rakwb eng RC254-282 Johanna Buschmann verfasserin aut Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Dorothea M. Heuberger verfasserin aut Fatma Kivrak Pfiffner verfasserin aut Petra Wolint verfasserin aut Jae-Hwi Jang verfasserin aut Wolfgang Jungraithmayr verfasserin aut Pietro Giovanoli verfasserin aut Maurizio Calcagni verfasserin aut Conny F. Waschkies verfasserin aut In Cancers MDPI AG, 2010 14(2022), 13, p 3114 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:14 year:2022 number:13, p 3114 https://doi.org/10.3390/cancers14133114 kostenfrei https://doaj.org/article/eb4f2f99aead47448998e63899b4cc5b kostenfrei https://www.mdpi.com/2072-6694/14/13/3114 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 13, p 3114 |
allfieldsGer |
10.3390/cancers14133114 doi (DE-627)DOAJ036875015 (DE-599)DOAJeb4f2f99aead47448998e63899b4cc5b DE-627 ger DE-627 rakwb eng RC254-282 Johanna Buschmann verfasserin aut Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Dorothea M. Heuberger verfasserin aut Fatma Kivrak Pfiffner verfasserin aut Petra Wolint verfasserin aut Jae-Hwi Jang verfasserin aut Wolfgang Jungraithmayr verfasserin aut Pietro Giovanoli verfasserin aut Maurizio Calcagni verfasserin aut Conny F. Waschkies verfasserin aut In Cancers MDPI AG, 2010 14(2022), 13, p 3114 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:14 year:2022 number:13, p 3114 https://doi.org/10.3390/cancers14133114 kostenfrei https://doaj.org/article/eb4f2f99aead47448998e63899b4cc5b kostenfrei https://www.mdpi.com/2072-6694/14/13/3114 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 13, p 3114 |
allfieldsSound |
10.3390/cancers14133114 doi (DE-627)DOAJ036875015 (DE-599)DOAJeb4f2f99aead47448998e63899b4cc5b DE-627 ger DE-627 rakwb eng RC254-282 Johanna Buschmann verfasserin aut Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Dorothea M. Heuberger verfasserin aut Fatma Kivrak Pfiffner verfasserin aut Petra Wolint verfasserin aut Jae-Hwi Jang verfasserin aut Wolfgang Jungraithmayr verfasserin aut Pietro Giovanoli verfasserin aut Maurizio Calcagni verfasserin aut Conny F. Waschkies verfasserin aut In Cancers MDPI AG, 2010 14(2022), 13, p 3114 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:14 year:2022 number:13, p 3114 https://doi.org/10.3390/cancers14133114 kostenfrei https://doaj.org/article/eb4f2f99aead47448998e63899b4cc5b kostenfrei https://www.mdpi.com/2072-6694/14/13/3114 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 13, p 3114 |
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English |
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In Cancers 14(2022), 13, p 3114 volume:14 year:2022 number:13, p 3114 |
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RC254-282 Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI chorioallantoic membrane (CAM) magnetic resonance imaging (MRI) A549 lung adenocarcinoma cell grafts H460 lung adenocarcinoma cell grafts MC-38 colon carcinoma cell grafts hypercapnia |
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misc RC254-282 misc chorioallantoic membrane (CAM) misc magnetic resonance imaging (MRI) misc A549 lung adenocarcinoma cell grafts misc H460 lung adenocarcinoma cell grafts misc MC-38 colon carcinoma cell grafts misc hypercapnia misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
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probing vasoreactivity and hypoxic phenotype in different tumor grafts grown on the chorioallantoic membrane of the chicken embryo in ovo using mri |
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Probing Vasoreactivity and Hypoxic Phenotype in Different Tumor Grafts Grown on the Chorioallantoic Membrane of the Chicken Embryo In Ovo Using MRI |
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Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. |
abstractGer |
Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. |
abstract_unstemmed |
Tumor grafts grown on the chorioallantoic membrane (CAM) of chicken embryos represent a transition between cell culture and mammalian in vivo models. Magnetic resonance imaging (MRI) started to harness this potential. Functional gas challenge is feasible on the CAM. Using quantitative T1 and T2* mapping, we characterized the response of MC-38 colon, A549, and H460 adeno-carcinoma cell grafts to hypercapnic (HC) and hypercapnic-hyperoxic (HCHO) gas challenges, pertaining to the grafts’ vascular and oxygenation phenotypes. MR imaging revealed that larger T1 and T2* were located in the center of H460 and MC-38 tumors. Quantitative analysis showed a significant reduction in T1 and a significant increase in T2* in response to HCHO for A549 grafts, while H460 and MC-38 tumors did not respond to either gas challenge. Different tumor grafts respond differentially to HC and HCHO conditions. A549 tumor grafts, with higher vessel density and smaller tumor diameter compared with H460 and MC-38 grafts, had a significant response in T1 for HCHO and T2* increased slightly during HC and significantly under HCHO, consistent with a normoxic phenotype and functional vasoreactivity. Therefore, gas challenges enable differential characterization of tumor grafts with respect to their vascular and oxygenation status. |
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