Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains

<p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological n...
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Gespeichert in:

Autor*in:	Landry Christian R [verfasserIn] Aubin-Horth Nadia [verfasserIn] Cavalieri Duccio [verfasserIn] De Filippo Carlotta [verfasserIn] Mengoni Alessio [verfasserIn] Giuntini Elisa [verfasserIn] Becker Anke [verfasserIn] Bazzicalupo Marco [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2005

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 6(2005), 1, p 158
Übergeordnetes Werk:	volume:6 ; year:2005 ; number:1, p 158

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-6-158

Katalog-ID:	DOAJ036991074

Internformat


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245	1	0	\|a Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
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520			\|a <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p<
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912			\|a GBV_ILN_4700
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allfields	10.1186/1471-2164-6-158 doi (DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Landry Christian R verfasserin aut Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p< Biotechnology Genetics Aubin-Horth Nadia verfasserin aut Cavalieri Duccio verfasserin aut De Filippo Carlotta verfasserin aut Mengoni Alessio verfasserin aut Giuntini Elisa verfasserin aut Becker Anke verfasserin aut Bazzicalupo Marco verfasserin aut In BMC Genomics BMC, 2003 6(2005), 1, p 158 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:6 year:2005 number:1, p 158 https://doi.org/10.1186/1471-2164-6-158 kostenfrei https://doaj.org/article/d5d9dc9271c84678a9f1c823d0460cf4 kostenfrei http://www.biomedcentral.com/1471-2164/6/158 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 158
spelling	10.1186/1471-2164-6-158 doi (DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Landry Christian R verfasserin aut Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p< Biotechnology Genetics Aubin-Horth Nadia verfasserin aut Cavalieri Duccio verfasserin aut De Filippo Carlotta verfasserin aut Mengoni Alessio verfasserin aut Giuntini Elisa verfasserin aut Becker Anke verfasserin aut Bazzicalupo Marco verfasserin aut In BMC Genomics BMC, 2003 6(2005), 1, p 158 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:6 year:2005 number:1, p 158 https://doi.org/10.1186/1471-2164-6-158 kostenfrei https://doaj.org/article/d5d9dc9271c84678a9f1c823d0460cf4 kostenfrei http://www.biomedcentral.com/1471-2164/6/158 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 158
allfields_unstemmed	10.1186/1471-2164-6-158 doi (DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Landry Christian R verfasserin aut Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p< Biotechnology Genetics Aubin-Horth Nadia verfasserin aut Cavalieri Duccio verfasserin aut De Filippo Carlotta verfasserin aut Mengoni Alessio verfasserin aut Giuntini Elisa verfasserin aut Becker Anke verfasserin aut Bazzicalupo Marco verfasserin aut In BMC Genomics BMC, 2003 6(2005), 1, p 158 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:6 year:2005 number:1, p 158 https://doi.org/10.1186/1471-2164-6-158 kostenfrei https://doaj.org/article/d5d9dc9271c84678a9f1c823d0460cf4 kostenfrei http://www.biomedcentral.com/1471-2164/6/158 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 158
allfieldsGer	10.1186/1471-2164-6-158 doi (DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Landry Christian R verfasserin aut Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p< Biotechnology Genetics Aubin-Horth Nadia verfasserin aut Cavalieri Duccio verfasserin aut De Filippo Carlotta verfasserin aut Mengoni Alessio verfasserin aut Giuntini Elisa verfasserin aut Becker Anke verfasserin aut Bazzicalupo Marco verfasserin aut In BMC Genomics BMC, 2003 6(2005), 1, p 158 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:6 year:2005 number:1, p 158 https://doi.org/10.1186/1471-2164-6-158 kostenfrei https://doaj.org/article/d5d9dc9271c84678a9f1c823d0460cf4 kostenfrei http://www.biomedcentral.com/1471-2164/6/158 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 158
allfieldsSound	10.1186/1471-2164-6-158 doi (DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Landry Christian R verfasserin aut Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p< Biotechnology Genetics Aubin-Horth Nadia verfasserin aut Cavalieri Duccio verfasserin aut De Filippo Carlotta verfasserin aut Mengoni Alessio verfasserin aut Giuntini Elisa verfasserin aut Becker Anke verfasserin aut Bazzicalupo Marco verfasserin aut In BMC Genomics BMC, 2003 6(2005), 1, p 158 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:6 year:2005 number:1, p 158 https://doi.org/10.1186/1471-2164-6-158 kostenfrei https://doaj.org/article/d5d9dc9271c84678a9f1c823d0460cf4 kostenfrei http://www.biomedcentral.com/1471-2164/6/158 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 158
language	English
source	In BMC Genomics 6(2005), 1, p 158 volume:6 year:2005 number:1, p 158
sourceStr	In BMC Genomics 6(2005), 1, p 158 volume:6 year:2005 number:1, p 158
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Landry Christian R @@aut@@ Aubin-Horth Nadia @@aut@@ Cavalieri Duccio @@aut@@ De Filippo Carlotta @@aut@@ Mengoni Alessio @@aut@@ Giuntini Elisa @@aut@@ Becker Anke @@aut@@ Bazzicalupo Marco @@aut@@
publishDateDaySort_date	2005-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ036991074
language_de	englisch
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callnumber-first	T - Technology
author	Landry Christian R
spellingShingle	Landry Christian R misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
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topic_title	TP248.13-248.65 QH426-470 Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
topic	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
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title	Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
ctrlnum	(DE-627)DOAJ036991074 (DE-599)DOAJd5d9dc9271c84678a9f1c823d0460cf4
title_full	Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
author_sort	Landry Christian R
journal	BMC Genomics
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author_browse	Landry Christian R Aubin-Horth Nadia Cavalieri Duccio De Filippo Carlotta Mengoni Alessio Giuntini Elisa Becker Anke Bazzicalupo Marco
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author-letter	Landry Christian R
doi_str_mv	10.1186/1471-2164-6-158
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title_sort	large-scale genetic variation of the symbiosis-required megaplasmid psyma revealed by comparative genomic analysis of <it<sinorhizobium meliloti </it<natural strains
callnumber	TP248.13-248.65
title_auth	Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
abstract	<p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<<it<Sinorhizobium meliloti </it<is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (<it<Medicago sativa</it<). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region.</p< <p<Results</p< <p<From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function.</p< <p<Conclusion</p< <p<The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in <it<S. meliloti</it<.</p<
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title_short	Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains
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Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of <it<Sinorhizobium meliloti </it<natural strains

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