HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways

Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid c...
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Autor*in:	Xin Zhou [verfasserIn] Ningou Huang [verfasserIn] Wenxin Chen [verfasserIn] Tang Xiaoling [verfasserIn] Behnam Mahdavi [verfasserIn] Amir Raoofi [verfasserIn] Davood Mahdian [verfasserIn] Hadi Atabati [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3

Übergeordnetes Werk:	In: AMB Express - SpringerOpen, 2011, 10(2020), 1, Seite 11
Übergeordnetes Werk:	volume:10 ; year:2020 ; number:1 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s13568-020-01138-9

Katalog-ID:	DOAJ037213482

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520			\|a Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells.
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allfields	10.1186/s13568-020-01138-9 doi (DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QR1-502 Xin Zhou verfasserin aut HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells. Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology Ningou Huang verfasserin aut Wenxin Chen verfasserin aut Tang Xiaoling verfasserin aut Behnam Mahdavi verfasserin aut Amir Raoofi verfasserin aut Davood Mahdian verfasserin aut Hadi Atabati verfasserin aut In AMB Express SpringerOpen, 2011 10(2020), 1, Seite 11 (DE-627)665672926 (DE-600)2621432-5 21910855 nnns volume:10 year:2020 number:1 pages:11 https://doi.org/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 kostenfrei http://link.springer.com/article/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/toc/2191-0855 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 1 11
spelling	10.1186/s13568-020-01138-9 doi (DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QR1-502 Xin Zhou verfasserin aut HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells. Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology Ningou Huang verfasserin aut Wenxin Chen verfasserin aut Tang Xiaoling verfasserin aut Behnam Mahdavi verfasserin aut Amir Raoofi verfasserin aut Davood Mahdian verfasserin aut Hadi Atabati verfasserin aut In AMB Express SpringerOpen, 2011 10(2020), 1, Seite 11 (DE-627)665672926 (DE-600)2621432-5 21910855 nnns volume:10 year:2020 number:1 pages:11 https://doi.org/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 kostenfrei http://link.springer.com/article/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/toc/2191-0855 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 1 11
allfields_unstemmed	10.1186/s13568-020-01138-9 doi (DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QR1-502 Xin Zhou verfasserin aut HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells. Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology Ningou Huang verfasserin aut Wenxin Chen verfasserin aut Tang Xiaoling verfasserin aut Behnam Mahdavi verfasserin aut Amir Raoofi verfasserin aut Davood Mahdian verfasserin aut Hadi Atabati verfasserin aut In AMB Express SpringerOpen, 2011 10(2020), 1, Seite 11 (DE-627)665672926 (DE-600)2621432-5 21910855 nnns volume:10 year:2020 number:1 pages:11 https://doi.org/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 kostenfrei http://link.springer.com/article/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/toc/2191-0855 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 1 11
allfieldsGer	10.1186/s13568-020-01138-9 doi (DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QR1-502 Xin Zhou verfasserin aut HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells. Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology Ningou Huang verfasserin aut Wenxin Chen verfasserin aut Tang Xiaoling verfasserin aut Behnam Mahdavi verfasserin aut Amir Raoofi verfasserin aut Davood Mahdian verfasserin aut Hadi Atabati verfasserin aut In AMB Express SpringerOpen, 2011 10(2020), 1, Seite 11 (DE-627)665672926 (DE-600)2621432-5 21910855 nnns volume:10 year:2020 number:1 pages:11 https://doi.org/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 kostenfrei http://link.springer.com/article/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/toc/2191-0855 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 1 11
allfieldsSound	10.1186/s13568-020-01138-9 doi (DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QR1-502 Xin Zhou verfasserin aut HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells. Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology Ningou Huang verfasserin aut Wenxin Chen verfasserin aut Tang Xiaoling verfasserin aut Behnam Mahdavi verfasserin aut Amir Raoofi verfasserin aut Davood Mahdian verfasserin aut Hadi Atabati verfasserin aut In AMB Express SpringerOpen, 2011 10(2020), 1, Seite 11 (DE-627)665672926 (DE-600)2621432-5 21910855 nnns volume:10 year:2020 number:1 pages:11 https://doi.org/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 kostenfrei http://link.springer.com/article/10.1186/s13568-020-01138-9 kostenfrei https://doaj.org/toc/2191-0855 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 1 11
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source	In AMB Express 10(2020), 1, Seite 11 volume:10 year:2020 number:1 pages:11
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topic_facet	Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3 Biotechnology Microbiology
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authorswithroles_txt_mv	Xin Zhou @@aut@@ Ningou Huang @@aut@@ Wenxin Chen @@aut@@ Tang Xiaoling @@aut@@ Behnam Mahdavi @@aut@@ Amir Raoofi @@aut@@ Davood Mahdian @@aut@@ Hadi Atabati @@aut@@
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topic_title	TP248.13-248.65 QR1-502 HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways Linum usitatissimum Phenolic profile Prostate cancer Induction apoptosis Bax Caspase-3
topic	misc TP248.13-248.65 misc QR1-502 misc Linum usitatissimum misc Phenolic profile misc Prostate cancer misc Induction apoptosis misc Bax misc Caspase-3 misc Biotechnology misc Microbiology
topic_unstemmed	misc TP248.13-248.65 misc QR1-502 misc Linum usitatissimum misc Phenolic profile misc Prostate cancer misc Induction apoptosis misc Bax misc Caspase-3 misc Biotechnology misc Microbiology
topic_browse	misc TP248.13-248.65 misc QR1-502 misc Linum usitatissimum misc Phenolic profile misc Prostate cancer misc Induction apoptosis misc Bax misc Caspase-3 misc Biotechnology misc Microbiology
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title	HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways
ctrlnum	(DE-627)DOAJ037213482 (DE-599)DOAJbbb8ece5f12140b69538a0ba2f52aa94
title_full	HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways
author_sort	Xin Zhou
journal	AMB Express
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author_browse	Xin Zhou Ningou Huang Wenxin Chen Tang Xiaoling Behnam Mahdavi Amir Raoofi Davood Mahdian Hadi Atabati
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class	TP248.13-248.65 QR1-502
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author-letter	Xin Zhou
doi_str_mv	10.1186/s13568-020-01138-9
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title_sort	hplc phenolic profile and induction of apoptosis by linum usitatissimum extract in lncap cells by caspase3 and bax pathways
callnumber	TP248.13-248.65
title_auth	HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways
abstract	Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells.
abstractGer	Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells.
abstract_unstemmed	Abstract Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells.
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title_short	HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways
url	https://doi.org/10.1186/s13568-020-01138-9 https://doaj.org/article/bbb8ece5f12140b69538a0ba2f52aa94 http://link.springer.com/article/10.1186/s13568-020-01138-9 https://doaj.org/toc/2191-0855
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author2	Ningou Huang Wenxin Chen Tang Xiaoling Behnam Mahdavi Amir Raoofi Davood Mahdian Hadi Atabati
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up_date	2024-07-04T00:21:45.709Z
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