The ribonuclease Dis3 is an essential regulator of the developmental transcriptome

<p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exo...
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Autor*in:	Hou Dezhi [verfasserIn] Ruiz Miriam [verfasserIn] Andrulis Erik D [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	Dis3 Ribonuclease RNase RNA metabolism RNA turnover

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 13(2012), 1, p 359
Übergeordnetes Werk:	volume:13 ; year:2012 ; number:1, p 359

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-13-359

Katalog-ID:	DOAJ037225553

Internformat


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520			\|a <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p<
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matchkey_str	article:14712164:2012----::hrbncesdsiaesnileuaooteeeom
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callnumber-subject-code	TP
publishDate	2012
allfields	10.1186/1471-2164-13-359 doi (DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hou Dezhi verfasserin aut The ribonuclease Dis3 is an essential regulator of the developmental transcriptome 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p< Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics Ruiz Miriam verfasserin aut Andrulis Erik D verfasserin aut In BMC Genomics BMC, 2003 13(2012), 1, p 359 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:13 year:2012 number:1, p 359 https://doi.org/10.1186/1471-2164-13-359 kostenfrei https://doaj.org/article/1ba9e28c67d24736a25fa408981bd21f kostenfrei http://www.biomedcentral.com/1471-2164/13/359 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1, p 359
spelling	10.1186/1471-2164-13-359 doi (DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hou Dezhi verfasserin aut The ribonuclease Dis3 is an essential regulator of the developmental transcriptome 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p< Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics Ruiz Miriam verfasserin aut Andrulis Erik D verfasserin aut In BMC Genomics BMC, 2003 13(2012), 1, p 359 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:13 year:2012 number:1, p 359 https://doi.org/10.1186/1471-2164-13-359 kostenfrei https://doaj.org/article/1ba9e28c67d24736a25fa408981bd21f kostenfrei http://www.biomedcentral.com/1471-2164/13/359 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1, p 359
allfields_unstemmed	10.1186/1471-2164-13-359 doi (DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hou Dezhi verfasserin aut The ribonuclease Dis3 is an essential regulator of the developmental transcriptome 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p< Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics Ruiz Miriam verfasserin aut Andrulis Erik D verfasserin aut In BMC Genomics BMC, 2003 13(2012), 1, p 359 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:13 year:2012 number:1, p 359 https://doi.org/10.1186/1471-2164-13-359 kostenfrei https://doaj.org/article/1ba9e28c67d24736a25fa408981bd21f kostenfrei http://www.biomedcentral.com/1471-2164/13/359 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1, p 359
allfieldsGer	10.1186/1471-2164-13-359 doi (DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hou Dezhi verfasserin aut The ribonuclease Dis3 is an essential regulator of the developmental transcriptome 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p< Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics Ruiz Miriam verfasserin aut Andrulis Erik D verfasserin aut In BMC Genomics BMC, 2003 13(2012), 1, p 359 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:13 year:2012 number:1, p 359 https://doi.org/10.1186/1471-2164-13-359 kostenfrei https://doaj.org/article/1ba9e28c67d24736a25fa408981bd21f kostenfrei http://www.biomedcentral.com/1471-2164/13/359 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1, p 359
allfieldsSound	10.1186/1471-2164-13-359 doi (DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hou Dezhi verfasserin aut The ribonuclease Dis3 is an essential regulator of the developmental transcriptome 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p< Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics Ruiz Miriam verfasserin aut Andrulis Erik D verfasserin aut In BMC Genomics BMC, 2003 13(2012), 1, p 359 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:13 year:2012 number:1, p 359 https://doi.org/10.1186/1471-2164-13-359 kostenfrei https://doaj.org/article/1ba9e28c67d24736a25fa408981bd21f kostenfrei http://www.biomedcentral.com/1471-2164/13/359 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1, p 359
language	English
source	In BMC Genomics 13(2012), 1, p 359 volume:13 year:2012 number:1, p 359
sourceStr	In BMC Genomics 13(2012), 1, p 359 volume:13 year:2012 number:1, p 359
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Hou Dezhi @@aut@@ Ruiz Miriam @@aut@@ Andrulis Erik D @@aut@@
publishDateDaySort_date	2012-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ037225553
language_de	englisch
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callnumber-first	T - Technology
author	Hou Dezhi
spellingShingle	Hou Dezhi misc TP248.13-248.65 misc QH426-470 misc Dis3 misc Ribonuclease misc RNase misc <it<Drosophila melanogaster</it< misc RNA metabolism misc RNA turnover misc Biotechnology misc Genetics The ribonuclease Dis3 is an essential regulator of the developmental transcriptome
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topic_title	TP248.13-248.65 QH426-470 The ribonuclease Dis3 is an essential regulator of the developmental transcriptome Dis3 Ribonuclease RNase <it<Drosophila melanogaster</it< RNA metabolism RNA turnover
topic	misc TP248.13-248.65 misc QH426-470 misc Dis3 misc Ribonuclease misc RNase misc <it<Drosophila melanogaster</it< misc RNA metabolism misc RNA turnover misc Biotechnology misc Genetics
topic_unstemmed	misc TP248.13-248.65 misc QH426-470 misc Dis3 misc Ribonuclease misc RNase misc <it<Drosophila melanogaster</it< misc RNA metabolism misc RNA turnover misc Biotechnology misc Genetics
topic_browse	misc TP248.13-248.65 misc QH426-470 misc Dis3 misc Ribonuclease misc RNase misc <it<Drosophila melanogaster</it< misc RNA metabolism misc RNA turnover misc Biotechnology misc Genetics
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	The ribonuclease Dis3 is an essential regulator of the developmental transcriptome
ctrlnum	(DE-627)DOAJ037225553 (DE-599)DOAJ1ba9e28c67d24736a25fa408981bd21f
title_full	The ribonuclease Dis3 is an essential regulator of the developmental transcriptome
author_sort	Hou Dezhi
journal	BMC Genomics
journalStr	BMC Genomics
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author_browse	Hou Dezhi Ruiz Miriam Andrulis Erik D
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title_sort	ribonuclease dis3 is an essential regulator of the developmental transcriptome
callnumber	TP248.13-248.65
title_auth	The ribonuclease Dis3 is an essential regulator of the developmental transcriptome
abstract	<p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Dis3 is ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. Evolutionarily conserved from eubacteria to eukaryotes and a crucial component of the RNA processing exosome, Dis3 has been shown to be essential in yeast and fly S2 cells. However, it is not known whether Dis3 has essential functions in a metazoan. This study inquires whether Dis3 is required for <it<Drosophila</it< development and viability and how Dis3 regulates the transcriptome in the developing fly.</p< <p<Results</p< <p<Using transgenic flies, we show that Dis3 knock down (Dis3KD) retards growth, induces melanotic tumor formation, and ultimately results in 2<sup<nd</sup< instar larval lethality. In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals. Bioinformatic analysis of transcripts from Dis3KD flies reveals substantial transcriptomic changes, most notably down-regulation in early expressed RNAs. Finally, gene ontology analysis of this early stage shows that Dis3 regulates transcripts related to extracellular structure and remodelling, neurogenesis, and nucleotide metabolism.</p< <p<Conclusions</p< <p<We conclude that Dis3 is essential for early <it<Drosophila melanogaster</it< development and has specific and important stage-specific roles in regulating RNA metabolism. In showing for the first time that Dis3 is required for the development of a multicellular organism, our work provides mechanistic insight into how Dis3—either independent of or associated with the RNA processing exosome—participates in cell type-specific RNA turnover in metazoan development.</p<
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title_short	The ribonuclease Dis3 is an essential regulator of the developmental transcriptome
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The ribonuclease Dis3 is an essential regulator of the developmental transcriptome

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