Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients

AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were s...
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Autor*in:	Shang-Li Jin [verfasserIn] Hai-Ke Guo [verfasserIn] Zhi-Hui Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract

Übergeordnetes Werk:	In: Guoji Yanke Zazhi - Press of International Journal of Ophthalmology (IJO PRESS), 2017, 15(2015), 6, Seite 968-971
Übergeordnetes Werk:	volume:15 ; year:2015 ; number:6 ; pages:968-971

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3980/j.issn.1672-5123.2015.6.07

Katalog-ID:	DOAJ037225677

Internformat


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520			\|a AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment.
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allfields	10.3980/j.issn.1672-5123.2015.6.07 doi (DE-627)DOAJ037225677 (DE-599)DOAJfd672d37ae24430f9040fe265e238a7c DE-627 ger DE-627 rakwb eng RE1-994 Shang-Li Jin verfasserin aut Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment. Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology Hai-Ke Guo verfasserin aut Zhi-Hui Chen verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 15(2015), 6, Seite 968-971 (DE-627)1680946854 16725123 nnns volume:15 year:2015 number:6 pages:968-971 https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 kostenfrei https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c kostenfrei http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_2817 AR 15 2015 6 968-971
spelling	10.3980/j.issn.1672-5123.2015.6.07 doi (DE-627)DOAJ037225677 (DE-599)DOAJfd672d37ae24430f9040fe265e238a7c DE-627 ger DE-627 rakwb eng RE1-994 Shang-Li Jin verfasserin aut Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment. Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology Hai-Ke Guo verfasserin aut Zhi-Hui Chen verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 15(2015), 6, Seite 968-971 (DE-627)1680946854 16725123 nnns volume:15 year:2015 number:6 pages:968-971 https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 kostenfrei https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c kostenfrei http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_2817 AR 15 2015 6 968-971
allfields_unstemmed	10.3980/j.issn.1672-5123.2015.6.07 doi (DE-627)DOAJ037225677 (DE-599)DOAJfd672d37ae24430f9040fe265e238a7c DE-627 ger DE-627 rakwb eng RE1-994 Shang-Li Jin verfasserin aut Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment. Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology Hai-Ke Guo verfasserin aut Zhi-Hui Chen verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 15(2015), 6, Seite 968-971 (DE-627)1680946854 16725123 nnns volume:15 year:2015 number:6 pages:968-971 https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 kostenfrei https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c kostenfrei http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_2817 AR 15 2015 6 968-971
allfieldsGer	10.3980/j.issn.1672-5123.2015.6.07 doi (DE-627)DOAJ037225677 (DE-599)DOAJfd672d37ae24430f9040fe265e238a7c DE-627 ger DE-627 rakwb eng RE1-994 Shang-Li Jin verfasserin aut Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment. Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology Hai-Ke Guo verfasserin aut Zhi-Hui Chen verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 15(2015), 6, Seite 968-971 (DE-627)1680946854 16725123 nnns volume:15 year:2015 number:6 pages:968-971 https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 kostenfrei https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c kostenfrei http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_2817 AR 15 2015 6 968-971
allfieldsSound	10.3980/j.issn.1672-5123.2015.6.07 doi (DE-627)DOAJ037225677 (DE-599)DOAJfd672d37ae24430f9040fe265e238a7c DE-627 ger DE-627 rakwb eng RE1-994 Shang-Li Jin verfasserin aut Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment. Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology Hai-Ke Guo verfasserin aut Zhi-Hui Chen verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 15(2015), 6, Seite 968-971 (DE-627)1680946854 16725123 nnns volume:15 year:2015 number:6 pages:968-971 https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 kostenfrei https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c kostenfrei http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_2817 AR 15 2015 6 968-971
language	English
source	In Guoji Yanke Zazhi 15(2015), 6, Seite 968-971 volume:15 year:2015 number:6 pages:968-971
sourceStr	In Guoji Yanke Zazhi 15(2015), 6, Seite 968-971 volume:15 year:2015 number:6 pages:968-971
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract Ophthalmology
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container_title	Guoji Yanke Zazhi
authorswithroles_txt_mv	Shang-Li Jin @@aut@@ Hai-Ke Guo @@aut@@ Zhi-Hui Chen @@aut@@
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RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. 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callnumber-first	R - Medicine
author	Shang-Li Jin
spellingShingle	Shang-Li Jin misc RE1-994 misc Sirtuin type l gene misc diabetic cataract misc age-related cataract misc traumatic cataract misc Ophthalmology Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients
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topic_title	RE1-994 Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients Sirtuin type l gene diabetic cataract age-related cataract traumatic cataract
topic	misc RE1-994 misc Sirtuin type l gene misc diabetic cataract misc age-related cataract misc traumatic cataract misc Ophthalmology
topic_unstemmed	misc RE1-994 misc Sirtuin type l gene misc diabetic cataract misc age-related cataract misc traumatic cataract misc Ophthalmology
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title	Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients
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title_full	Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients
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title_sort	preliminary study on expression of sirt1 gene in lens epithelial cells of diabetic cataract patients
callnumber	RE1-994
title_auth	Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients
abstract	AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment.
abstractGer	AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment.
abstract_unstemmed	AIM:To investigate the expression of Sirtuin type l(SIRT1)gene in lens epithelial cells(LECs)of diabetic cataract.<p<METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.<p<RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(<i<P</i<<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(<i<P</i<<0.05). <p<CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment.
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title_short	Preliminary study on expression of SIRT1 gene in lens epithelial cells of diabetic cataract patients
url	https://doi.org/10.3980/j.issn.1672-5123.2015.6.07 https://doaj.org/article/fd672d37ae24430f9040fe265e238a7c http://ies.ijo.cn/cn_publish/2015/6/201506007.pdf https://doaj.org/toc/1672-5123
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