Development of bioluminescent <it<Salmonella </it<strains for use in food safety

<p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperat...
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Autor*in:	Bailey R Hartford [verfasserIn] Wills Robert [verfasserIn] Kirkpatrick Tasha B [verfasserIn] Howe Kevin [verfasserIn] Karsi Attila [verfasserIn] Lawrence Mark L [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Übergeordnetes Werk:	In: BMC Microbiology - BMC, 2003, 8(2008), 1, p 10
Übergeordnetes Werk:	volume:8 ; year:2008 ; number:1, p 10

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2180-8-10

Katalog-ID:	DOAJ037259377

Internformat


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912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
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912			\|a GBV_ILN_4322
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author_variant	b r h brh w r wr k t b ktb h k hk k a ka l m l lml
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publishDate	2008
allfields	10.1186/1471-2180-8-10 doi (DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54 DE-627 ger DE-627 rakwb eng QR1-502 Bailey R Hartford verfasserin aut Development of bioluminescent <it<Salmonella </it<strains for use in food safety 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p< Microbiology Wills Robert verfasserin aut Kirkpatrick Tasha B verfasserin aut Howe Kevin verfasserin aut Karsi Attila verfasserin aut Lawrence Mark L verfasserin aut In BMC Microbiology BMC, 2003 8(2008), 1, p 10 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:8 year:2008 number:1, p 10 https://doi.org/10.1186/1471-2180-8-10 kostenfrei https://doaj.org/article/51c793e3edb04416bf7bc8dc163ede54 kostenfrei http://www.biomedcentral.com/1471-2180/8/10 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1, p 10
spelling	10.1186/1471-2180-8-10 doi (DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54 DE-627 ger DE-627 rakwb eng QR1-502 Bailey R Hartford verfasserin aut Development of bioluminescent <it<Salmonella </it<strains for use in food safety 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p< Microbiology Wills Robert verfasserin aut Kirkpatrick Tasha B verfasserin aut Howe Kevin verfasserin aut Karsi Attila verfasserin aut Lawrence Mark L verfasserin aut In BMC Microbiology BMC, 2003 8(2008), 1, p 10 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:8 year:2008 number:1, p 10 https://doi.org/10.1186/1471-2180-8-10 kostenfrei https://doaj.org/article/51c793e3edb04416bf7bc8dc163ede54 kostenfrei http://www.biomedcentral.com/1471-2180/8/10 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1, p 10
allfields_unstemmed	10.1186/1471-2180-8-10 doi (DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54 DE-627 ger DE-627 rakwb eng QR1-502 Bailey R Hartford verfasserin aut Development of bioluminescent <it<Salmonella </it<strains for use in food safety 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p< Microbiology Wills Robert verfasserin aut Kirkpatrick Tasha B verfasserin aut Howe Kevin verfasserin aut Karsi Attila verfasserin aut Lawrence Mark L verfasserin aut In BMC Microbiology BMC, 2003 8(2008), 1, p 10 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:8 year:2008 number:1, p 10 https://doi.org/10.1186/1471-2180-8-10 kostenfrei https://doaj.org/article/51c793e3edb04416bf7bc8dc163ede54 kostenfrei http://www.biomedcentral.com/1471-2180/8/10 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1, p 10
allfieldsGer	10.1186/1471-2180-8-10 doi (DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54 DE-627 ger DE-627 rakwb eng QR1-502 Bailey R Hartford verfasserin aut Development of bioluminescent <it<Salmonella </it<strains for use in food safety 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p< Microbiology Wills Robert verfasserin aut Kirkpatrick Tasha B verfasserin aut Howe Kevin verfasserin aut Karsi Attila verfasserin aut Lawrence Mark L verfasserin aut In BMC Microbiology BMC, 2003 8(2008), 1, p 10 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:8 year:2008 number:1, p 10 https://doi.org/10.1186/1471-2180-8-10 kostenfrei https://doaj.org/article/51c793e3edb04416bf7bc8dc163ede54 kostenfrei http://www.biomedcentral.com/1471-2180/8/10 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1, p 10
allfieldsSound	10.1186/1471-2180-8-10 doi (DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54 DE-627 ger DE-627 rakwb eng QR1-502 Bailey R Hartford verfasserin aut Development of bioluminescent <it<Salmonella </it<strains for use in food safety 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p< Microbiology Wills Robert verfasserin aut Kirkpatrick Tasha B verfasserin aut Howe Kevin verfasserin aut Karsi Attila verfasserin aut Lawrence Mark L verfasserin aut In BMC Microbiology BMC, 2003 8(2008), 1, p 10 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:8 year:2008 number:1, p 10 https://doi.org/10.1186/1471-2180-8-10 kostenfrei https://doaj.org/article/51c793e3edb04416bf7bc8dc163ede54 kostenfrei http://www.biomedcentral.com/1471-2180/8/10 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1, p 10
language	English
source	In BMC Microbiology 8(2008), 1, p 10 volume:8 year:2008 number:1, p 10
sourceStr	In BMC Microbiology 8(2008), 1, p 10 volume:8 year:2008 number:1, p 10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microbiology
isfreeaccess_bool	true
container_title	BMC Microbiology
authorswithroles_txt_mv	Bailey R Hartford @@aut@@ Wills Robert @@aut@@ Kirkpatrick Tasha B @@aut@@ Howe Kevin @@aut@@ Karsi Attila @@aut@@ Lawrence Mark L @@aut@@
publishDateDaySort_date	2008-01-01T00:00:00Z
hierarchy_top_id	326644997
id	DOAJ037259377
language_de	englisch
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callnumber-first	Q - Science
author	Bailey R Hartford
spellingShingle	Bailey R Hartford misc QR1-502 misc Microbiology Development of bioluminescent <it<Salmonella </it<strains for use in food safety
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topic_title	QR1-502 Development of bioluminescent <it<Salmonella </it<strains for use in food safety
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title	Development of bioluminescent <it<Salmonella </it<strains for use in food safety
ctrlnum	(DE-627)DOAJ037259377 (DE-599)DOAJ51c793e3edb04416bf7bc8dc163ede54
title_full	Development of bioluminescent <it<Salmonella </it<strains for use in food safety
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author_browse	Bailey R Hartford Wills Robert Kirkpatrick Tasha B Howe Kevin Karsi Attila Lawrence Mark L
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title_sort	development of bioluminescent <it<salmonella </it<strains for use in food safety
callnumber	QR1-502
title_auth	Development of bioluminescent <it<Salmonella </it<strains for use in food safety
abstract	<p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<<it<Salmonella </it<can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent <it<Salmonella </it<strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve <it<Salmonella </it<strains from the broiler production continuum were transformed with the broad host range plasmid pAK<it<lux</it<1, and a chicken skin attachment model was developed.</p< <p<Results</p< <p<<it<Salmonella </it<strains carrying pAK<it<lux</it<1 constitutively expressed the <it<luxCDABE </it<operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent <it<Salmonella </it<strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of <it<Salmonella </it<strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain.</p< <p<Conclusion</p< <p<This study demonstrated that bioluminescence is a sensitive and effective tool to detect <it<Salmonella </it<on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing <it<Salmonella </it<contamination on food products.</p<
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title_short	Development of bioluminescent <it<Salmonella </it<strains for use in food safety
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Development of bioluminescent <it<Salmonella </it<strains for use in food safety

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