Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	van't Westende Wendy PC [verfasserIn] America Antoine HP [verfasserIn] Cordewener Jan HG [verfasserIn] Schenk Martijn F [verfasserIn] Smulders Marinus JM [verfasserIn] Gilissen Luud JWJ [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: BMC Plant Biology - BMC, 2003, 9(2009), 1, p 24
Übergeordnetes Werk:	volume:9 ; year:2009 ; number:1, p 24

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2229-9-24

Katalog-ID:	DOAJ037468383

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100	0		\|a van't Westende Wendy PC \|e verfasserin \|4 aut
245	1	0	\|a Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
264		1	\|c 2009
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520			\|a <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p<
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700	0		\|a America Antoine HP \|e verfasserin \|4 aut
700	0		\|a Cordewener Jan HG \|e verfasserin \|4 aut
700	0		\|a Schenk Martijn F \|e verfasserin \|4 aut
700	0		\|a Smulders Marinus JM \|e verfasserin \|4 aut
700	0		\|a Gilissen Luud JWJ \|e verfasserin \|4 aut
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912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
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912			\|a GBV_ILN_2011
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912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 9 \|j 2009 \|e 1, p 24

Indexfelder

author_variant	v w w p vwwp a a h aah c j h cjh s m f smf s m j smj g l j glj
matchkey_str	article:14712229:2009----::hrceiainfr0eefoegteuapceadeetoobt
hierarchy_sort_str	2009
callnumber-subject-code	QK
publishDate	2009
allfields	10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24
spelling	10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24
allfields_unstemmed	10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24
allfieldsGer	10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24
allfieldsSound	10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24
language	English
source	In BMC Plant Biology 9(2009), 1, p 24 volume:9 year:2009 number:1, p 24
sourceStr	In BMC Plant Biology 9(2009), 1, p 24 volume:9 year:2009 number:1, p 24
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Botany
isfreeaccess_bool	true
container_title	BMC Plant Biology
authorswithroles_txt_mv	van't Westende Wendy PC @@aut@@ America Antoine HP @@aut@@ Cordewener Jan HG @@aut@@ Schenk Martijn F @@aut@@ Smulders Marinus JM @@aut@@ Gilissen Luud JWJ @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	335489060
id	DOAJ037468383
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ037468383</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230308010622.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2009 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1471-2229-9-24</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ037468383</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ9967908054f940c5a1dc478135a7b957</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QK1-989</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">van't Westende Wendy PC</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. 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author	van't Westende Wendy PC
spellingShingle	van't Westende Wendy PC misc QK1-989 misc Botany Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
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topic_title	QK1-989 Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
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title	Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
ctrlnum	(DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957
title_full	Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
author_sort	van't Westende Wendy PC
journal	BMC Plant Biology
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author_browse	van't Westende Wendy PC America Antoine HP Cordewener Jan HG Schenk Martijn F Smulders Marinus JM Gilissen Luud JWJ
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author-letter	van't Westende Wendy PC
doi_str_mv	10.1186/1471-2229-9-24
author2-role	verfasserin
title_sort	characterization of pr-10 genes from eight betula species and detection of bet v 1 isoforms in birch pollen
callnumber	QK1-989
title_auth	Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
abstract	<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p<
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title_short	Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
url	https://doi.org/10.1186/1471-2229-9-24 https://doaj.org/article/9967908054f940c5a1dc478135a7b957 http://www.biomedcentral.com/1471-2229/9/24 https://doaj.org/toc/1471-2229
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Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. 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Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

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