Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen
<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species...
Ausführliche Beschreibung
Autor*in: |
van't Westende Wendy PC [verfasserIn] America Antoine HP [verfasserIn] Cordewener Jan HG [verfasserIn] Schenk Martijn F [verfasserIn] Smulders Marinus JM [verfasserIn] Gilissen Luud JWJ [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2009 |
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Übergeordnetes Werk: |
In: BMC Plant Biology - BMC, 2003, 9(2009), 1, p 24 |
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Übergeordnetes Werk: |
volume:9 ; year:2009 ; number:1, p 24 |
Links: |
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DOI / URN: |
10.1186/1471-2229-9-24 |
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Katalog-ID: |
DOAJ037468383 |
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520 | |a <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< | ||
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10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24 |
spelling |
10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24 |
allfields_unstemmed |
10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24 |
allfieldsGer |
10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24 |
allfieldsSound |
10.1186/1471-2229-9-24 doi (DE-627)DOAJ037468383 (DE-599)DOAJ9967908054f940c5a1dc478135a7b957 DE-627 ger DE-627 rakwb eng QK1-989 van't Westende Wendy PC verfasserin aut Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< Botany America Antoine HP verfasserin aut Cordewener Jan HG verfasserin aut Schenk Martijn F verfasserin aut Smulders Marinus JM verfasserin aut Gilissen Luud JWJ verfasserin aut In BMC Plant Biology BMC, 2003 9(2009), 1, p 24 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:9 year:2009 number:1, p 24 https://doi.org/10.1186/1471-2229-9-24 kostenfrei https://doaj.org/article/9967908054f940c5a1dc478135a7b957 kostenfrei http://www.biomedcentral.com/1471-2229/9/24 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 24 |
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Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. 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QK1-989 Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
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Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
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Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
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characterization of pr-10 genes from eight betula species and detection of bet v 1 isoforms in birch pollen |
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Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
abstract |
<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< |
abstractGer |
<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< |
abstract_unstemmed |
<p<Abstract</p< <p<Background</p< <p<Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch <it<(Betula pendula) </it<have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus <it<Betula</it<. Q-TOF LC-MS<sup<E </sup<was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.</p< <p<Results</p< <p<All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except <it<B. lenta</it<, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in <it<B. pendula </it<and its closest relatives.</p< <p<Conclusion</p< <p<Q-TOF LC-MS<sup<E </sup<allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. <it<B. pendula </it<contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of <it<B. lenta</it<, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.</p< |
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Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
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score |
7.400218 |