Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica
Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC reg...
Ausführliche Beschreibung
Autor*in: |
Chang Liu [verfasserIn] Chuchu Li [verfasserIn] Yuhuang Chen [verfasserIn] Huijing Hao [verfasserIn] Junrong Liang [verfasserIn] Ran Duan [verfasserIn] Zhaoke Guo [verfasserIn] Jing Zhang [verfasserIn] Zhongzhi Zhao [verfasserIn] Huaiqi Jing [verfasserIn] Xin Wang [verfasserIn] Shihe Shao [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2017 |
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Übergeordnetes Werk: |
In: Frontiers in Cellular and Infection Microbiology - Frontiers Media S.A., 2016, 7(2017) |
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Übergeordnetes Werk: |
volume:7 ; year:2017 |
Links: |
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DOI / URN: |
10.3389/fcimb.2017.00425 |
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Katalog-ID: |
DOAJ038232618 |
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10.3389/fcimb.2017.00425 doi (DE-627)DOAJ038232618 (DE-599)DOAJ7506a696a8fb4194a7167c0cae023687 DE-627 ger DE-627 rakwb eng QR1-502 Chang Liu verfasserin aut Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. Yersinia enteocolitica AmpC β-lactamase AmpD PBPs NagZ AmpR Microbiology Chang Liu verfasserin aut Chuchu Li verfasserin aut Chuchu Li verfasserin aut Yuhuang Chen verfasserin aut Huijing Hao verfasserin aut Junrong Liang verfasserin aut Ran Duan verfasserin aut Zhaoke Guo verfasserin aut Jing Zhang verfasserin aut Zhongzhi Zhao verfasserin aut Huaiqi Jing verfasserin aut Xin Wang verfasserin aut Shihe Shao verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 7(2017) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:7 year:2017 https://doi.org/10.3389/fcimb.2017.00425 kostenfrei https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 kostenfrei http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2017 |
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10.3389/fcimb.2017.00425 doi (DE-627)DOAJ038232618 (DE-599)DOAJ7506a696a8fb4194a7167c0cae023687 DE-627 ger DE-627 rakwb eng QR1-502 Chang Liu verfasserin aut Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. Yersinia enteocolitica AmpC β-lactamase AmpD PBPs NagZ AmpR Microbiology Chang Liu verfasserin aut Chuchu Li verfasserin aut Chuchu Li verfasserin aut Yuhuang Chen verfasserin aut Huijing Hao verfasserin aut Junrong Liang verfasserin aut Ran Duan verfasserin aut Zhaoke Guo verfasserin aut Jing Zhang verfasserin aut Zhongzhi Zhao verfasserin aut Huaiqi Jing verfasserin aut Xin Wang verfasserin aut Shihe Shao verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 7(2017) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:7 year:2017 https://doi.org/10.3389/fcimb.2017.00425 kostenfrei https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 kostenfrei http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2017 |
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10.3389/fcimb.2017.00425 doi (DE-627)DOAJ038232618 (DE-599)DOAJ7506a696a8fb4194a7167c0cae023687 DE-627 ger DE-627 rakwb eng QR1-502 Chang Liu verfasserin aut Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. Yersinia enteocolitica AmpC β-lactamase AmpD PBPs NagZ AmpR Microbiology Chang Liu verfasserin aut Chuchu Li verfasserin aut Chuchu Li verfasserin aut Yuhuang Chen verfasserin aut Huijing Hao verfasserin aut Junrong Liang verfasserin aut Ran Duan verfasserin aut Zhaoke Guo verfasserin aut Jing Zhang verfasserin aut Zhongzhi Zhao verfasserin aut Huaiqi Jing verfasserin aut Xin Wang verfasserin aut Shihe Shao verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 7(2017) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:7 year:2017 https://doi.org/10.3389/fcimb.2017.00425 kostenfrei https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 kostenfrei http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2017 |
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10.3389/fcimb.2017.00425 doi (DE-627)DOAJ038232618 (DE-599)DOAJ7506a696a8fb4194a7167c0cae023687 DE-627 ger DE-627 rakwb eng QR1-502 Chang Liu verfasserin aut Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. Yersinia enteocolitica AmpC β-lactamase AmpD PBPs NagZ AmpR Microbiology Chang Liu verfasserin aut Chuchu Li verfasserin aut Chuchu Li verfasserin aut Yuhuang Chen verfasserin aut Huijing Hao verfasserin aut Junrong Liang verfasserin aut Ran Duan verfasserin aut Zhaoke Guo verfasserin aut Jing Zhang verfasserin aut Zhongzhi Zhao verfasserin aut Huaiqi Jing verfasserin aut Xin Wang verfasserin aut Shihe Shao verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 7(2017) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:7 year:2017 https://doi.org/10.3389/fcimb.2017.00425 kostenfrei https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 kostenfrei http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2017 |
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10.3389/fcimb.2017.00425 doi (DE-627)DOAJ038232618 (DE-599)DOAJ7506a696a8fb4194a7167c0cae023687 DE-627 ger DE-627 rakwb eng QR1-502 Chang Liu verfasserin aut Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. Yersinia enteocolitica AmpC β-lactamase AmpD PBPs NagZ AmpR Microbiology Chang Liu verfasserin aut Chuchu Li verfasserin aut Chuchu Li verfasserin aut Yuhuang Chen verfasserin aut Huijing Hao verfasserin aut Junrong Liang verfasserin aut Ran Duan verfasserin aut Zhaoke Guo verfasserin aut Jing Zhang verfasserin aut Zhongzhi Zhao verfasserin aut Huaiqi Jing verfasserin aut Xin Wang verfasserin aut Shihe Shao verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 7(2017) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:7 year:2017 https://doi.org/10.3389/fcimb.2017.00425 kostenfrei https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 kostenfrei http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2017 |
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Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica |
abstract |
Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. |
abstractGer |
Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. |
abstract_unstemmed |
Yersinia enterocolitica encodes a chromosomal AmpC β-lactamase under the regulation of the classical ampR-ampC system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in ampC regulation of Y. enterocolitica, series of single/multiple mutant strains were systematically constructed and the ampC expression levels were determined by luxCDABE reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of ampC expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 (pbp4, pbp5, pbp6, and pbp7 inactivated) is approached to the YEΔD123 (ampD1, ampD2, and ampD3 inactivated). Deletion of nagZ gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7 ampC derepression phenotype but dispensable for YEΔD123. AmpR is essential for ampC hyperproduction in these two types of strains, inactivation of AmpR notable reduced the ampC expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7. |
collection_details |
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title_short |
Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica |
url |
https://doi.org/10.3389/fcimb.2017.00425 https://doaj.org/article/7506a696a8fb4194a7167c0cae023687 http://journal.frontiersin.org/article/10.3389/fcimb.2017.00425/full https://doaj.org/toc/2235-2988 |
remote_bool |
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author2 |
Chang Liu Chuchu Li Yuhuang Chen Huijing Hao Junrong Liang Ran Duan Zhaoke Guo Jing Zhang Zhongzhi Zhao Huaiqi Jing Xin Wang Shihe Shao |
author2Str |
Chang Liu Chuchu Li Yuhuang Chen Huijing Hao Junrong Liang Ran Duan Zhaoke Guo Jing Zhang Zhongzhi Zhao Huaiqi Jing Xin Wang Shihe Shao |
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doi_str |
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callnumber-a |
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up_date |
2024-07-03T16:48:47.447Z |
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