Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction

Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Luo L [verfasserIn] Chen B [verfasserIn] Huang Y [verfasserIn] Liang Z [verfasserIn] Li S [verfasserIn] Yin Y [verfasserIn] Chen J [verfasserIn] Wu W [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	placental growth factor L-arginine acute myocardial infarction angiogenesis

Übergeordnetes Werk:	In: Drug Design, Development and Therapy - Dove Medical Press, 2008, (2016), Seite 3483-3492
Übergeordnetes Werk:	year:2016 ; pages:3483-3492

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ038364646

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520			\|a Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis
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allfields	(DE-627)DOAJ038364646 (DE-599)DOAJ5be31c31cdea4aceb374cb58a641a31c DE-627 ger DE-627 rakwb eng RM1-950 Luo L verfasserin aut Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology Chen B verfasserin aut Huang Y verfasserin aut Liang Z verfasserin aut Li S verfasserin aut Yin Y verfasserin aut Chen J verfasserin aut Wu W verfasserin aut In Drug Design, Development and Therapy Dove Medical Press, 2008 (2016), Seite 3483-3492 (DE-627)578533138 (DE-600)2451346-5 11778881 nnns year:2016 pages:3483-3492 https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c kostenfrei https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT kostenfrei https://doaj.org/toc/1177-8881 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3483-3492
spelling	(DE-627)DOAJ038364646 (DE-599)DOAJ5be31c31cdea4aceb374cb58a641a31c DE-627 ger DE-627 rakwb eng RM1-950 Luo L verfasserin aut Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology Chen B verfasserin aut Huang Y verfasserin aut Liang Z verfasserin aut Li S verfasserin aut Yin Y verfasserin aut Chen J verfasserin aut Wu W verfasserin aut In Drug Design, Development and Therapy Dove Medical Press, 2008 (2016), Seite 3483-3492 (DE-627)578533138 (DE-600)2451346-5 11778881 nnns year:2016 pages:3483-3492 https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c kostenfrei https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT kostenfrei https://doaj.org/toc/1177-8881 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3483-3492
allfields_unstemmed	(DE-627)DOAJ038364646 (DE-599)DOAJ5be31c31cdea4aceb374cb58a641a31c DE-627 ger DE-627 rakwb eng RM1-950 Luo L verfasserin aut Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology Chen B verfasserin aut Huang Y verfasserin aut Liang Z verfasserin aut Li S verfasserin aut Yin Y verfasserin aut Chen J verfasserin aut Wu W verfasserin aut In Drug Design, Development and Therapy Dove Medical Press, 2008 (2016), Seite 3483-3492 (DE-627)578533138 (DE-600)2451346-5 11778881 nnns year:2016 pages:3483-3492 https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c kostenfrei https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT kostenfrei https://doaj.org/toc/1177-8881 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3483-3492
allfieldsGer	(DE-627)DOAJ038364646 (DE-599)DOAJ5be31c31cdea4aceb374cb58a641a31c DE-627 ger DE-627 rakwb eng RM1-950 Luo L verfasserin aut Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology Chen B verfasserin aut Huang Y verfasserin aut Liang Z verfasserin aut Li S verfasserin aut Yin Y verfasserin aut Chen J verfasserin aut Wu W verfasserin aut In Drug Design, Development and Therapy Dove Medical Press, 2008 (2016), Seite 3483-3492 (DE-627)578533138 (DE-600)2451346-5 11778881 nnns year:2016 pages:3483-3492 https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c kostenfrei https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT kostenfrei https://doaj.org/toc/1177-8881 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3483-3492
allfieldsSound	(DE-627)DOAJ038364646 (DE-599)DOAJ5be31c31cdea4aceb374cb58a641a31c DE-627 ger DE-627 rakwb eng RM1-950 Luo L verfasserin aut Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology Chen B verfasserin aut Huang Y verfasserin aut Liang Z verfasserin aut Li S verfasserin aut Yin Y verfasserin aut Chen J verfasserin aut Wu W verfasserin aut In Drug Design, Development and Therapy Dove Medical Press, 2008 (2016), Seite 3483-3492 (DE-627)578533138 (DE-600)2451346-5 11778881 nnns year:2016 pages:3483-3492 https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c kostenfrei https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT kostenfrei https://doaj.org/toc/1177-8881 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3483-3492
language	English
source	In Drug Design, Development and Therapy (2016), Seite 3483-3492 year:2016 pages:3483-3492
sourceStr	In Drug Design, Development and Therapy (2016), Seite 3483-3492 year:2016 pages:3483-3492
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	placental growth factor L-arginine acute myocardial infarction angiogenesis Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Drug Design, Development and Therapy
authorswithroles_txt_mv	Luo L @@aut@@ Chen B @@aut@@ Huang Y @@aut@@ Liang Z @@aut@@ Li S @@aut@@ Yin Y @@aut@@ Chen J @@aut@@ Wu W @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	578533138
id	DOAJ038364646
language_de	englisch
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The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson&rsquo;s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P&lt;0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P&lt;0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P&lt;0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P&lt;0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P&lt;0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. 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callnumber-first	R - Medicine
author	Luo L
spellingShingle	Luo L misc RM1-950 misc placental growth factor misc L-arginine misc acute myocardial infarction misc angiogenesis misc Therapeutics. Pharmacology Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction
authorStr	Luo L
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topic_title	RM1-950 Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction placental growth factor L-arginine acute myocardial infarction angiogenesis
topic	misc RM1-950 misc placental growth factor misc L-arginine misc acute myocardial infarction misc angiogenesis misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc placental growth factor misc L-arginine misc acute myocardial infarction misc angiogenesis misc Therapeutics. Pharmacology
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title	Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction
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title_full	Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction
author_sort	Luo L
journal	Drug Design, Development and Therapy
journalStr	Drug Design, Development and Therapy
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author_browse	Luo L Chen B Huang Y Liang Z Li S Yin Y Chen J Wu W
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author-letter	Luo L
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title_sort	cardioprotective activity of placental growth factor combined with oral supplementation of l-arginine in a rat model of acute myocardial infarction
callnumber	RM1-950
title_auth	Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction
abstract	Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis
abstractGer	Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis
abstract_unstemmed	Liyun Luo,1,* Bairong Chen,1,* Yin Huang,1 Zibin Liang,2 Songbiao Li,1 Yuelan Yin,1 Jian Chen,1 Wei Wu1 1Department of Cardiology, 2Department of Oncological Radiotherapy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workObjective: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with L-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and L-arginine with those of direct administration of PlGF alone in a rat model of AMI.Materials and methods: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, L-arginine group, PlGF group, and combination group (PlGF + L-arginine). An AMI rat model was established by ligation of the left anterior descending of coronary arteries. After 4 weeks of postligation treatment, cardiac function, scar area, angiogenesis and arteriogenesis, myocardial endothelial nitric oxide synthase (eNOS) and collagen I protein content, and plasma concentration of brain natriuretic peptide (BNP) were studied. Echocardiography, Masson’s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P<0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P<0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P<0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P<0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P<0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. L-arginine increases the expression of the eNOS protein. PlGF and L-arginine have a more pronounced, synergistic protective effect on myocardial protection compared with that of exogenous PlGF therapy alone. Keywords: placental growth factor, L-arginine, acute myocardial infarction, angiogenesis
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title_short	Cardioprotective activity of placental growth factor combined with oral supplementation of L-arginine in a rat model of acute myocardial infarction
url	https://doaj.org/article/5be31c31cdea4aceb374cb58a641a31c https://www.dovepress.com/cardioprotective-activity-of-placental-growth-factor-combined-with-ora-peer-reviewed-article-DDDT https://doaj.org/toc/1177-8881
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author2	Chen B Huang Y Liang Z Li S Yin Y Chen J Wu W
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up_date	2024-07-03T17:32:21.598Z
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Echocardiography, Masson&rsquo;s staining, immunohistochemical analyses, Western blot, and enzyme-linked immunosorbent assay were performed.Results: Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and capillary and arteriole densities were higher in the PlGF group than in the normal saline group (P&lt;0.01). Scar area, collagen I protein content, and plasma concentration of BNP were decreased in the PlGF group (P&lt;0.01). Myocardial eNOS protein level was elevated in the L-arginine group and PlGF + L-arginine group (P&lt;0.01). Compared with the PlGF group, LVEF, LVFS, myocardial eNOS, and capillary and arteriole densities were higher in the combination group (P&lt;0.01). Scar area, content of collagen I protein, and plasma concentration of BNP were reduced in the combination group (P&lt;0.01).Conclusion: Exogenous administration of PlGF stimulates angiogenesis and improves cardiac function. 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