Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with p...
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Autor*in:	X.Z. Cai [verfasserIn] W.Y. Huang [verfasserIn] Y. Qiao [verfasserIn] Y. Chen [verfasserIn] S.Y. Du [verfasserIn] D. Chen [verfasserIn] S. Yu [verfasserIn] N. Liu [verfasserIn] L.Y. Dou [verfasserIn] Y. Jiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell

Übergeordnetes Werk:	In: Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica, 2004, 48(2015), 1, Seite 77-82
Übergeordnetes Werk:	volume:48 ; year:2015 ; number:1 ; pages:77-82

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/1414-431X20143701

Katalog-ID:	DOAJ038386992

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allfields	10.1590/1414-431X20143701 doi (DE-627)DOAJ038386992 (DE-599)DOAJ5fa9808cc1734521bbb00c9eb46d0cdf DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 X.Z. Cai verfasserin aut Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE. TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General) W.Y. Huang verfasserin aut Y. Qiao verfasserin aut Y. Chen verfasserin aut S.Y. Du verfasserin aut D. Chen verfasserin aut S. Yu verfasserin aut N. Liu verfasserin aut L.Y. Dou verfasserin aut Y. Jiang verfasserin aut In Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica, 2004 48(2015), 1, Seite 77-82 (DE-627)324613407 (DE-600)2028749-5 1414431X nnns volume:48 year:2015 number:1 pages:77-82 https://doi.org/10.1590/1414-431X20143701 kostenfrei https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en kostenfrei https://doaj.org/toc/1414-431X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2015 1 77-82
spelling	10.1590/1414-431X20143701 doi (DE-627)DOAJ038386992 (DE-599)DOAJ5fa9808cc1734521bbb00c9eb46d0cdf DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 X.Z. Cai verfasserin aut Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE. TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General) W.Y. Huang verfasserin aut Y. Qiao verfasserin aut Y. Chen verfasserin aut S.Y. Du verfasserin aut D. Chen verfasserin aut S. Yu verfasserin aut N. Liu verfasserin aut L.Y. Dou verfasserin aut Y. Jiang verfasserin aut In Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica, 2004 48(2015), 1, Seite 77-82 (DE-627)324613407 (DE-600)2028749-5 1414431X nnns volume:48 year:2015 number:1 pages:77-82 https://doi.org/10.1590/1414-431X20143701 kostenfrei https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en kostenfrei https://doaj.org/toc/1414-431X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2015 1 77-82
allfields_unstemmed	10.1590/1414-431X20143701 doi (DE-627)DOAJ038386992 (DE-599)DOAJ5fa9808cc1734521bbb00c9eb46d0cdf DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 X.Z. Cai verfasserin aut Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE. TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General) W.Y. Huang verfasserin aut Y. Qiao verfasserin aut Y. Chen verfasserin aut S.Y. Du verfasserin aut D. Chen verfasserin aut S. Yu verfasserin aut N. Liu verfasserin aut L.Y. Dou verfasserin aut Y. Jiang verfasserin aut In Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica, 2004 48(2015), 1, Seite 77-82 (DE-627)324613407 (DE-600)2028749-5 1414431X nnns volume:48 year:2015 number:1 pages:77-82 https://doi.org/10.1590/1414-431X20143701 kostenfrei https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en kostenfrei https://doaj.org/toc/1414-431X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2015 1 77-82
allfieldsGer	10.1590/1414-431X20143701 doi (DE-627)DOAJ038386992 (DE-599)DOAJ5fa9808cc1734521bbb00c9eb46d0cdf DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 X.Z. Cai verfasserin aut Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE. TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General) W.Y. Huang verfasserin aut Y. Qiao verfasserin aut Y. Chen verfasserin aut S.Y. Du verfasserin aut D. Chen verfasserin aut S. Yu verfasserin aut N. Liu verfasserin aut L.Y. Dou verfasserin aut Y. Jiang verfasserin aut In Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica, 2004 48(2015), 1, Seite 77-82 (DE-627)324613407 (DE-600)2028749-5 1414431X nnns volume:48 year:2015 number:1 pages:77-82 https://doi.org/10.1590/1414-431X20143701 kostenfrei https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en kostenfrei https://doaj.org/toc/1414-431X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2015 1 77-82
allfieldsSound	10.1590/1414-431X20143701 doi (DE-627)DOAJ038386992 (DE-599)DOAJ5fa9808cc1734521bbb00c9eb46d0cdf DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 X.Z. Cai verfasserin aut Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE. TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General) W.Y. Huang verfasserin aut Y. Qiao verfasserin aut Y. Chen verfasserin aut S.Y. Du verfasserin aut D. Chen verfasserin aut S. Yu verfasserin aut N. Liu verfasserin aut L.Y. Dou verfasserin aut Y. Jiang verfasserin aut In Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica, 2004 48(2015), 1, Seite 77-82 (DE-627)324613407 (DE-600)2028749-5 1414431X nnns volume:48 year:2015 number:1 pages:77-82 https://doi.org/10.1590/1414-431X20143701 kostenfrei https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en kostenfrei https://doaj.org/toc/1414-431X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2015 1 77-82
language	English
source	In Brazilian Journal of Medical and Biological Research 48(2015), 1, Seite 77-82 volume:48 year:2015 number:1 pages:77-82
sourceStr	In Brazilian Journal of Medical and Biological Research 48(2015), 1, Seite 77-82 volume:48 year:2015 number:1 pages:77-82
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institution	findex.gbv.de
topic_facet	TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell Medicine (General) Biology (General)
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container_title	Brazilian Journal of Medical and Biological Research
authorswithroles_txt_mv	X.Z. Cai @@aut@@ W.Y. Huang @@aut@@ Y. Qiao @@aut@@ Y. Chen @@aut@@ S.Y. Du @@aut@@ D. Chen @@aut@@ S. Yu @@aut@@ N. Liu @@aut@@ L.Y. Dou @@aut@@ Y. Jiang @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	324613407
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callnumber-first	R - Medicine
author	X.Z. Cai
spellingShingle	X.Z. Cai misc R5-920 misc QH301-705.5 misc TIM-3 misc Systemic lupus erythematosus misc Lupus nephritis misc T-helper cell misc Medicine (General) misc Biology (General) Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
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topic_title	R5-920 QH301-705.5 Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus TIM-3 Systemic lupus erythematosus Lupus nephritis T-helper cell
topic	misc R5-920 misc QH301-705.5 misc TIM-3 misc Systemic lupus erythematosus misc Lupus nephritis misc T-helper cell misc Medicine (General) misc Biology (General)
topic_unstemmed	misc R5-920 misc QH301-705.5 misc TIM-3 misc Systemic lupus erythematosus misc Lupus nephritis misc T-helper cell misc Medicine (General) misc Biology (General)
topic_browse	misc R5-920 misc QH301-705.5 misc TIM-3 misc Systemic lupus erythematosus misc Lupus nephritis misc T-helper cell misc Medicine (General) misc Biology (General)
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title	Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
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title_full	Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
author_sort	X.Z. Cai
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author_browse	X.Z. Cai W.Y. Huang Y. Qiao Y. Chen S.Y. Du D. Chen S. Yu N. Liu L.Y. Dou Y. Jiang
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title_sort	downregulation of tim-3 mrna expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
callnumber	R5-920
title_auth	Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
abstract	The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE.
abstractGer	The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE.
abstract_unstemmed	The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients compared with healthy controls (P<0.001). No significant differences in TIM family protein expression were observed in leukocytes from SLE patients and healthy controls (P<0.05). SLE patients with lupus nephritis (LN) had a significantly lower expression of TIM-3 mRNA than those without LN (P=0.001). There was no significant difference in the expression of TIM-3 mRNA within different classes of LN (P<0.05). Correlation of TIM-3 mRNA expression with serum IgA was highly significant (r=0.425, P=0.004), but was weakly correlated with total serum protein (rs=0.283, P=0.049) and serum albumin (rs=0.297, P=0.047). TIM-3 mRNA expression was weakly correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI; rs=-0.272, P=0.032). Our results suggest that below-normal expression of TIM-3 mRNA in PBMC may be involved in the pathogenesis of SLE.
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title_short	Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
url	https://doi.org/10.1590/1414-431X20143701 https://doaj.org/article/5fa9808cc1734521bbb00c9eb46d0cdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077&lng=en&tlng=en https://doaj.org/toc/1414-431X
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