Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?

<b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA),...
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Autor*in:	Das Sujata [verfasserIn] Sharma Savitri [verfasserIn] Kar Sarita [verfasserIn] Sahu Srikant [verfasserIn] Samal Bikash [verfasserIn] Mallick Aparajita [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar

Übergeordnetes Werk:	In: Indian Journal of Ophthalmology - Wolters Kluwer Medknow Publications, 2005, 58(2010), 4, Seite 281-286
Übergeordnetes Werk:	volume:58 ; year:2010 ; number:4 ; pages:281-286

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Katalog-ID:	DOAJ038544075

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520			\|a <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
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allfields	(DE-627)DOAJ038544075 (DE-599)DOAJ3e84f5a3f2324662b3c0cc52fb8350e1 DE-627 ger DE-627 rakwb eng RE1-994 Das Sujata verfasserin aut Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology Sharma Savitri verfasserin aut Kar Sarita verfasserin aut Sahu Srikant verfasserin aut Samal Bikash verfasserin aut Mallick Aparajita verfasserin aut In Indian Journal of Ophthalmology Wolters Kluwer Medknow Publications, 2005 58(2010), 4, Seite 281-286 (DE-627)485243563 (DE-600)2185999-1 19983689 nnns volume:58 year:2010 number:4 pages:281-286 https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 kostenfrei http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das kostenfrei https://doaj.org/toc/0301-4738 Journal toc kostenfrei https://doaj.org/toc/1998-3689 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2010 4 281-286
spelling	(DE-627)DOAJ038544075 (DE-599)DOAJ3e84f5a3f2324662b3c0cc52fb8350e1 DE-627 ger DE-627 rakwb eng RE1-994 Das Sujata verfasserin aut Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology Sharma Savitri verfasserin aut Kar Sarita verfasserin aut Sahu Srikant verfasserin aut Samal Bikash verfasserin aut Mallick Aparajita verfasserin aut In Indian Journal of Ophthalmology Wolters Kluwer Medknow Publications, 2005 58(2010), 4, Seite 281-286 (DE-627)485243563 (DE-600)2185999-1 19983689 nnns volume:58 year:2010 number:4 pages:281-286 https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 kostenfrei http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das kostenfrei https://doaj.org/toc/0301-4738 Journal toc kostenfrei https://doaj.org/toc/1998-3689 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2010 4 281-286
allfields_unstemmed	(DE-627)DOAJ038544075 (DE-599)DOAJ3e84f5a3f2324662b3c0cc52fb8350e1 DE-627 ger DE-627 rakwb eng RE1-994 Das Sujata verfasserin aut Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology Sharma Savitri verfasserin aut Kar Sarita verfasserin aut Sahu Srikant verfasserin aut Samal Bikash verfasserin aut Mallick Aparajita verfasserin aut In Indian Journal of Ophthalmology Wolters Kluwer Medknow Publications, 2005 58(2010), 4, Seite 281-286 (DE-627)485243563 (DE-600)2185999-1 19983689 nnns volume:58 year:2010 number:4 pages:281-286 https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 kostenfrei http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das kostenfrei https://doaj.org/toc/0301-4738 Journal toc kostenfrei https://doaj.org/toc/1998-3689 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2010 4 281-286
allfieldsGer	(DE-627)DOAJ038544075 (DE-599)DOAJ3e84f5a3f2324662b3c0cc52fb8350e1 DE-627 ger DE-627 rakwb eng RE1-994 Das Sujata verfasserin aut Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology Sharma Savitri verfasserin aut Kar Sarita verfasserin aut Sahu Srikant verfasserin aut Samal Bikash verfasserin aut Mallick Aparajita verfasserin aut In Indian Journal of Ophthalmology Wolters Kluwer Medknow Publications, 2005 58(2010), 4, Seite 281-286 (DE-627)485243563 (DE-600)2185999-1 19983689 nnns volume:58 year:2010 number:4 pages:281-286 https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 kostenfrei http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das kostenfrei https://doaj.org/toc/0301-4738 Journal toc kostenfrei https://doaj.org/toc/1998-3689 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2010 4 281-286
allfieldsSound	(DE-627)DOAJ038544075 (DE-599)DOAJ3e84f5a3f2324662b3c0cc52fb8350e1 DE-627 ger DE-627 rakwb eng RE1-994 Das Sujata verfasserin aut Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology Sharma Savitri verfasserin aut Kar Sarita verfasserin aut Sahu Srikant verfasserin aut Samal Bikash verfasserin aut Mallick Aparajita verfasserin aut In Indian Journal of Ophthalmology Wolters Kluwer Medknow Publications, 2005 58(2010), 4, Seite 281-286 (DE-627)485243563 (DE-600)2185999-1 19983689 nnns volume:58 year:2010 number:4 pages:281-286 https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 kostenfrei http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das kostenfrei https://doaj.org/toc/0301-4738 Journal toc kostenfrei https://doaj.org/toc/1998-3689 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2010 4 281-286
language	English
source	In Indian Journal of Ophthalmology 58(2010), 4, Seite 281-286 volume:58 year:2010 number:4 pages:281-286
sourceStr	In Indian Journal of Ophthalmology 58(2010), 4, Seite 281-286 volume:58 year:2010 number:4 pages:281-286
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar Ophthalmology
isfreeaccess_bool	true
container_title	Indian Journal of Ophthalmology
authorswithroles_txt_mv	Das Sujata @@aut@@ Sharma Savitri @@aut@@ Kar Sarita @@aut@@ Sahu Srikant @@aut@@ Samal Bikash @@aut@@ Mallick Aparajita @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	485243563
id	DOAJ038544075
language_de	englisch
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callnumber-first	R - Medicine
author	Das Sujata
spellingShingle	Das Sujata misc RE1-994 misc Blood agar misc chocolate agar misc diagnosis misc fungal keratitis misc Sabouraud dextrose agar misc Ophthalmology Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
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topic_title	RE1-994 Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar
topic	misc RE1-994 misc Blood agar misc chocolate agar misc diagnosis misc fungal keratitis misc Sabouraud dextrose agar misc Ophthalmology
topic_unstemmed	misc RE1-994 misc Blood agar misc chocolate agar misc diagnosis misc fungal keratitis misc Sabouraud dextrose agar misc Ophthalmology
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title	Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
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title_full	Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
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author_browse	Das Sujata Sharma Savitri Kar Sarita Sahu Srikant Samal Bikash Mallick Aparajita
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title_sort	is inclusion of sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
callnumber	RE1-994
title_auth	Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
abstract	<b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
abstractGer	<b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
abstract_unstemmed	<b<Purpose:</b< To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b< Materials and Methods:</b< Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i<Escherichia coli </i<overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b< Results:</b< Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i<P</i< = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i<in vitro</i< experiment although the characteristic spores and color were seen earlier on SDA. <b< Conclusion:</b< Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
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title_short	Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
url	https://doaj.org/article/3e84f5a3f2324662b3c0cc52fb8350e1 http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das https://doaj.org/toc/0301-4738 https://doaj.org/toc/1998-3689
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