Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro

This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fernanda Vidigal Duarte Souza [verfasserIn] Ana Maria Mascarenhas Eloy Canto [verfasserIn] Antônio da Silva Souza [verfasserIn] Maria Angélica Pereira de Carvalho Costa [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Spanisch ; Portugiesisch

Erschienen:	2010

Schlagwörter:	Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation

Übergeordnetes Werk:	In: Revista Brasileira de Fruticultura - Sociedade Brasileira de Fruticultura, 2004, 32(2010), 2, Seite 612-617
Übergeordnetes Werk:	volume:32 ; year:2010 ; number:2 ; pages:612-617

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ038767082

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allfields	(DE-627)DOAJ038767082 (DE-599)DOAJc70e49e8d8da45c3a75f8bcd52d70ba8 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Fernanda Vidigal Duarte Souza verfasserin aut Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture Ana Maria Mascarenhas Eloy Canto verfasserin aut Antônio da Silva Souza verfasserin aut Maria Angélica Pereira de Carvalho Costa verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 32(2010), 2, Seite 612-617 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns volume:32 year:2010 number:2 pages:612-617 https://doaj.org/article/c70e49e8d8da45c3a75f8bcd52d70ba8 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 kostenfrei https://doaj.org/toc/0100-2945 Journal toc kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 32 2010 2 612-617
spelling	(DE-627)DOAJ038767082 (DE-599)DOAJc70e49e8d8da45c3a75f8bcd52d70ba8 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Fernanda Vidigal Duarte Souza verfasserin aut Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture Ana Maria Mascarenhas Eloy Canto verfasserin aut Antônio da Silva Souza verfasserin aut Maria Angélica Pereira de Carvalho Costa verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 32(2010), 2, Seite 612-617 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns volume:32 year:2010 number:2 pages:612-617 https://doaj.org/article/c70e49e8d8da45c3a75f8bcd52d70ba8 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 kostenfrei https://doaj.org/toc/0100-2945 Journal toc kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 32 2010 2 612-617
allfields_unstemmed	(DE-627)DOAJ038767082 (DE-599)DOAJc70e49e8d8da45c3a75f8bcd52d70ba8 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Fernanda Vidigal Duarte Souza verfasserin aut Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture Ana Maria Mascarenhas Eloy Canto verfasserin aut Antônio da Silva Souza verfasserin aut Maria Angélica Pereira de Carvalho Costa verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 32(2010), 2, Seite 612-617 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns volume:32 year:2010 number:2 pages:612-617 https://doaj.org/article/c70e49e8d8da45c3a75f8bcd52d70ba8 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 kostenfrei https://doaj.org/toc/0100-2945 Journal toc kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 32 2010 2 612-617
allfieldsGer	(DE-627)DOAJ038767082 (DE-599)DOAJc70e49e8d8da45c3a75f8bcd52d70ba8 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Fernanda Vidigal Duarte Souza verfasserin aut Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture Ana Maria Mascarenhas Eloy Canto verfasserin aut Antônio da Silva Souza verfasserin aut Maria Angélica Pereira de Carvalho Costa verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 32(2010), 2, Seite 612-617 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns volume:32 year:2010 number:2 pages:612-617 https://doaj.org/article/c70e49e8d8da45c3a75f8bcd52d70ba8 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 kostenfrei https://doaj.org/toc/0100-2945 Journal toc kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 32 2010 2 612-617
allfieldsSound	(DE-627)DOAJ038767082 (DE-599)DOAJc70e49e8d8da45c3a75f8bcd52d70ba8 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Fernanda Vidigal Duarte Souza verfasserin aut Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi. Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture Ana Maria Mascarenhas Eloy Canto verfasserin aut Antônio da Silva Souza verfasserin aut Maria Angélica Pereira de Carvalho Costa verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 32(2010), 2, Seite 612-617 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns volume:32 year:2010 number:2 pages:612-617 https://doaj.org/article/c70e49e8d8da45c3a75f8bcd52d70ba8 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 kostenfrei https://doaj.org/toc/0100-2945 Journal toc kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 32 2010 2 612-617
language	English Spanish Portuguese
source	In Revista Brasileira de Fruticultura 32(2010), 2, Seite 612-617 volume:32 year:2010 number:2 pages:612-617
sourceStr	In Revista Brasileira de Fruticultura 32(2010), 2, Seite 612-617 volume:32 year:2010 number:2 pages:612-617
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation Plant culture
isfreeaccess_bool	true
container_title	Revista Brasileira de Fruticultura
authorswithroles_txt_mv	Fernanda Vidigal Duarte Souza @@aut@@ Ana Maria Mascarenhas Eloy Canto @@aut@@ Antônio da Silva Souza @@aut@@ Maria Angélica Pereira de Carvalho Costa @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	363749667
id	DOAJ038767082
language_de	englisch spanisch portugiesisch
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callnumber-first	S - Agriculture
author	Fernanda Vidigal Duarte Souza
spellingShingle	Fernanda Vidigal Duarte Souza misc SB1-1110 misc Ananas comosus var. comosus misc cultura in vitro misc reguladores vegetais misc segmentos nodais misc conservação de germoplasma misc micropropagação misc in vitro culture misc plant regulators misc nodal segments misc germplasm conservation misc micropropagation misc Plant culture Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
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topic_title	SB1-1110 Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro Ananas comosus var. comosus cultura in vitro reguladores vegetais segmentos nodais conservação de germoplasma micropropagação in vitro culture plant regulators nodal segments germplasm conservation micropropagation
topic	misc SB1-1110 misc Ananas comosus var. comosus misc cultura in vitro misc reguladores vegetais misc segmentos nodais misc conservação de germoplasma misc micropropagação misc in vitro culture misc plant regulators misc nodal segments misc germplasm conservation misc micropropagation misc Plant culture
topic_unstemmed	misc SB1-1110 misc Ananas comosus var. comosus misc cultura in vitro misc reguladores vegetais misc segmentos nodais misc conservação de germoplasma misc micropropagação misc in vitro culture misc plant regulators misc nodal segments misc germplasm conservation misc micropropagation misc Plant culture
topic_browse	misc SB1-1110 misc Ananas comosus var. comosus misc cultura in vitro misc reguladores vegetais misc segmentos nodais misc conservação de germoplasma misc micropropagação misc in vitro culture misc plant regulators misc nodal segments misc germplasm conservation misc micropropagation misc Plant culture
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title	Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
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title_full	Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
author_sort	Fernanda Vidigal Duarte Souza
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author_browse	Fernanda Vidigal Duarte Souza Ana Maria Mascarenhas Eloy Canto Antônio da Silva Souza Maria Angélica Pereira de Carvalho Costa
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title_sort	residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
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title_auth	Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
abstract	This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi.
abstractGer	This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi.
abstract_unstemmed	This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. O tratamento que resultou ser mais efetivo para o estiolamento das plantas foi o que apresentou maior dificuldade de regeneração, demonstrando o efeito residual do regulador auxina na regeneração de plantas de híbrido de abacaxi.
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title_short	Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro
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The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.<br<O objetivo deste trabalho foi avaliar a influência dos reguladores vegetais ácido naftalenoacético (ANA) e ácido giberélico (AG3) no estiolamento in vitro e posterior regeneração de plantas do abacaxizeiro híbrido PE x SC-60. Segmentos nodais de plantas produzidas in vitro, com aproximadamente 5-7 cm de comprimento, foram incubados em meio de cultura MS em sua concentração básica, suplementados com 0,0; 0,5 e 1,0 mg L-1 de ácido naftalenoacético (ANA) em combinação com ácido giberélico (AG3), nas concentrações de 0,0; 0,5 e 1,0 mg L-1, e mantidos a 27ºC e sob condições de escuro. As avaliações foram realizadas aos 90; 180 e 270 dias de incubação. Os melhores resultados foram obtidos com a concentração de 1,0 mg L-1 de ANA. No experimento posterior de regeneração, hastes com 3 cm, oriundas dos tratamentos de estiolamento foram cultivadas em meio de proliferação, quando aos 60 dias de cultivo avaliou-se o número de plantas regeneradas por tratamento. 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Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Efeito residual de reguladores de crescimento no estiolamento e regeneração de plantas de abacaxi in vitro

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