Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results

Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this...
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Gespeichert in:

Autor*in:	Edvin Ingberg [verfasserIn] Hua Dock [verfasserIn] Elvar Theodorsson [verfasserIn] Annette Theodorsson [verfasserIn] Jakob O. Ström [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability

Übergeordnetes Werk:	In: BMC Neuroscience - BMC, 2003, 19(2018), 1, Seite 11
Übergeordnetes Werk:	volume:19 ; year:2018 ; number:1 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12868-018-0425-0

Katalog-ID:	DOAJ03888299X

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520			\|a Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive.
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650		4	\|a Middle cerebral artery occlusion
650		4	\|a Ischemic stroke
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650		4	\|a Mortality
650		4	\|a Variability
653		0	\|a Neurosciences. Biological psychiatry. Neuropsychiatry
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allfields	10.1186/s12868-018-0425-0 doi (DE-627)DOAJ03888299X (DE-599)DOAJdbff28c4ccc14d7b948af50817673b1a DE-627 ger DE-627 rakwb eng RC321-571 QP351-495 Edvin Ingberg verfasserin aut Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive. Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology Hua Dock verfasserin aut Elvar Theodorsson verfasserin aut Annette Theodorsson verfasserin aut Jakob O. Ström verfasserin aut In BMC Neuroscience BMC, 2003 19(2018), 1, Seite 11 (DE-627)326643648 (DE-600)2041344-0 14712202 nnns volume:19 year:2018 number:1 pages:11 https://doi.org/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a kostenfrei http://link.springer.com/article/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/toc/1471-2202 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 11
spelling	10.1186/s12868-018-0425-0 doi (DE-627)DOAJ03888299X (DE-599)DOAJdbff28c4ccc14d7b948af50817673b1a DE-627 ger DE-627 rakwb eng RC321-571 QP351-495 Edvin Ingberg verfasserin aut Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive. Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology Hua Dock verfasserin aut Elvar Theodorsson verfasserin aut Annette Theodorsson verfasserin aut Jakob O. Ström verfasserin aut In BMC Neuroscience BMC, 2003 19(2018), 1, Seite 11 (DE-627)326643648 (DE-600)2041344-0 14712202 nnns volume:19 year:2018 number:1 pages:11 https://doi.org/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a kostenfrei http://link.springer.com/article/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/toc/1471-2202 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 11
allfields_unstemmed	10.1186/s12868-018-0425-0 doi (DE-627)DOAJ03888299X (DE-599)DOAJdbff28c4ccc14d7b948af50817673b1a DE-627 ger DE-627 rakwb eng RC321-571 QP351-495 Edvin Ingberg verfasserin aut Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive. Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology Hua Dock verfasserin aut Elvar Theodorsson verfasserin aut Annette Theodorsson verfasserin aut Jakob O. Ström verfasserin aut In BMC Neuroscience BMC, 2003 19(2018), 1, Seite 11 (DE-627)326643648 (DE-600)2041344-0 14712202 nnns volume:19 year:2018 number:1 pages:11 https://doi.org/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a kostenfrei http://link.springer.com/article/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/toc/1471-2202 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 11
allfieldsGer	10.1186/s12868-018-0425-0 doi (DE-627)DOAJ03888299X (DE-599)DOAJdbff28c4ccc14d7b948af50817673b1a DE-627 ger DE-627 rakwb eng RC321-571 QP351-495 Edvin Ingberg verfasserin aut Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive. Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology Hua Dock verfasserin aut Elvar Theodorsson verfasserin aut Annette Theodorsson verfasserin aut Jakob O. Ström verfasserin aut In BMC Neuroscience BMC, 2003 19(2018), 1, Seite 11 (DE-627)326643648 (DE-600)2041344-0 14712202 nnns volume:19 year:2018 number:1 pages:11 https://doi.org/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a kostenfrei http://link.springer.com/article/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/toc/1471-2202 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 11
allfieldsSound	10.1186/s12868-018-0425-0 doi (DE-627)DOAJ03888299X (DE-599)DOAJdbff28c4ccc14d7b948af50817673b1a DE-627 ger DE-627 rakwb eng RC321-571 QP351-495 Edvin Ingberg verfasserin aut Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive. Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology Hua Dock verfasserin aut Elvar Theodorsson verfasserin aut Annette Theodorsson verfasserin aut Jakob O. Ström verfasserin aut In BMC Neuroscience BMC, 2003 19(2018), 1, Seite 11 (DE-627)326643648 (DE-600)2041344-0 14712202 nnns volume:19 year:2018 number:1 pages:11 https://doi.org/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a kostenfrei http://link.springer.com/article/10.1186/s12868-018-0425-0 kostenfrei https://doaj.org/toc/1471-2202 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 11
language	English
source	In BMC Neuroscience 19(2018), 1, Seite 11 volume:19 year:2018 number:1 pages:11
sourceStr	In BMC Neuroscience 19(2018), 1, Seite 11 volume:19 year:2018 number:1 pages:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability Neurosciences. Biological psychiatry. Neuropsychiatry Neurophysiology and neuropsychology
isfreeaccess_bool	true
container_title	BMC Neuroscience
authorswithroles_txt_mv	Edvin Ingberg @@aut@@ Hua Dock @@aut@@ Elvar Theodorsson @@aut@@ Annette Theodorsson @@aut@@ Jakob O. Ström @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	326643648
id	DOAJ03888299X
language_de	englisch
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callnumber-first	R - Medicine
author	Edvin Ingberg
spellingShingle	Edvin Ingberg misc RC321-571 misc QP351-495 misc Rats misc Middle cerebral artery occlusion misc Ischemic stroke misc Laser Doppler flowmetry misc Mortality misc Variability misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Neurophysiology and neuropsychology Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
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topic_title	RC321-571 QP351-495 Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results Rats Middle cerebral artery occlusion Ischemic stroke Laser Doppler flowmetry Mortality Variability
topic	misc RC321-571 misc QP351-495 misc Rats misc Middle cerebral artery occlusion misc Ischemic stroke misc Laser Doppler flowmetry misc Mortality misc Variability misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Neurophysiology and neuropsychology
topic_unstemmed	misc RC321-571 misc QP351-495 misc Rats misc Middle cerebral artery occlusion misc Ischemic stroke misc Laser Doppler flowmetry misc Mortality misc Variability misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Neurophysiology and neuropsychology
topic_browse	misc RC321-571 misc QP351-495 misc Rats misc Middle cerebral artery occlusion misc Ischemic stroke misc Laser Doppler flowmetry misc Mortality misc Variability misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Neurophysiology and neuropsychology
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title	Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
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title_full	Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
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author_browse	Edvin Ingberg Hua Dock Elvar Theodorsson Annette Theodorsson Jakob O. Ström
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author-letter	Edvin Ingberg
doi_str_mv	10.1186/s12868-018-0425-0
author2-role	verfasserin
title_sort	effect of laser doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
callnumber	RC321-571
title_auth	Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
abstract	Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive.
abstractGer	Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive.
abstract_unstemmed	Abstract Background Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test. Results Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity. Conclusions Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive.
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title_short	Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results
url	https://doi.org/10.1186/s12868-018-0425-0 https://doaj.org/article/dbff28c4ccc14d7b948af50817673b1a http://link.springer.com/article/10.1186/s12868-018-0425-0 https://doaj.org/toc/1471-2202
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Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results

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Nicht das Richtige dabei?