Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanism...
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Autor*in:	Linlin Song [verfasserIn] Ximeng Han [verfasserIn] Cunyu Jia [verfasserIn] Xin Zhang [verfasserIn] Yunjie Jiao [verfasserIn] Taofeng Du [verfasserIn] Shuqi Xiao [verfasserIn] Julian A. Hiscox [verfasserIn] En-Min Zhou [verfasserIn] Yang Mu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53

Übergeordnetes Werk:	In: Virology Journal - BMC, 2004, 15(2018), 1, Seite 15
Übergeordnetes Werk:	volume:15 ; year:2018 ; number:1 ; pages:15

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12985-018-1081-9

Katalog-ID:	DOAJ03900354X

Internformat


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520			\|a Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis.
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912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
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912			\|a GBV_ILN_4325
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912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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Indexfelder

author_variant	l s ls x h xh c j cj x z xz y j yj t d td s x sx j a h jah e m z emz y m ym
matchkey_str	article:1743422X:2018----::ocnrpoutvadeprtrsnrmvrsniisac4poieainiidcnaotssngmrety
hierarchy_sort_str	2018
callnumber-subject-code	RC
publishDate	2018
allfields	10.1186/s12985-018-1081-9 doi (DE-627)DOAJ03900354X (DE-599)DOAJf1ff5f2f007f463db974bff1aaff80c1 DE-627 ger DE-627 rakwb eng RC109-216 Linlin Song verfasserin aut Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis. Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases Ximeng Han verfasserin aut Cunyu Jia verfasserin aut Xin Zhang verfasserin aut Yunjie Jiao verfasserin aut Taofeng Du verfasserin aut Shuqi Xiao verfasserin aut Julian A. Hiscox verfasserin aut En-Min Zhou verfasserin aut Yang Mu verfasserin aut In Virology Journal BMC, 2004 15(2018), 1, Seite 15 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:15 year:2018 number:1 pages:15 https://doi.org/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 kostenfrei http://link.springer.com/article/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 15
spelling	10.1186/s12985-018-1081-9 doi (DE-627)DOAJ03900354X (DE-599)DOAJf1ff5f2f007f463db974bff1aaff80c1 DE-627 ger DE-627 rakwb eng RC109-216 Linlin Song verfasserin aut Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis. Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases Ximeng Han verfasserin aut Cunyu Jia verfasserin aut Xin Zhang verfasserin aut Yunjie Jiao verfasserin aut Taofeng Du verfasserin aut Shuqi Xiao verfasserin aut Julian A. Hiscox verfasserin aut En-Min Zhou verfasserin aut Yang Mu verfasserin aut In Virology Journal BMC, 2004 15(2018), 1, Seite 15 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:15 year:2018 number:1 pages:15 https://doi.org/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 kostenfrei http://link.springer.com/article/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 15
allfields_unstemmed	10.1186/s12985-018-1081-9 doi (DE-627)DOAJ03900354X (DE-599)DOAJf1ff5f2f007f463db974bff1aaff80c1 DE-627 ger DE-627 rakwb eng RC109-216 Linlin Song verfasserin aut Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis. Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases Ximeng Han verfasserin aut Cunyu Jia verfasserin aut Xin Zhang verfasserin aut Yunjie Jiao verfasserin aut Taofeng Du verfasserin aut Shuqi Xiao verfasserin aut Julian A. Hiscox verfasserin aut En-Min Zhou verfasserin aut Yang Mu verfasserin aut In Virology Journal BMC, 2004 15(2018), 1, Seite 15 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:15 year:2018 number:1 pages:15 https://doi.org/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 kostenfrei http://link.springer.com/article/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 15
allfieldsGer	10.1186/s12985-018-1081-9 doi (DE-627)DOAJ03900354X (DE-599)DOAJf1ff5f2f007f463db974bff1aaff80c1 DE-627 ger DE-627 rakwb eng RC109-216 Linlin Song verfasserin aut Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis. Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases Ximeng Han verfasserin aut Cunyu Jia verfasserin aut Xin Zhang verfasserin aut Yunjie Jiao verfasserin aut Taofeng Du verfasserin aut Shuqi Xiao verfasserin aut Julian A. Hiscox verfasserin aut En-Min Zhou verfasserin aut Yang Mu verfasserin aut In Virology Journal BMC, 2004 15(2018), 1, Seite 15 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:15 year:2018 number:1 pages:15 https://doi.org/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 kostenfrei http://link.springer.com/article/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 15
allfieldsSound	10.1186/s12985-018-1081-9 doi (DE-627)DOAJ03900354X (DE-599)DOAJf1ff5f2f007f463db974bff1aaff80c1 DE-627 ger DE-627 rakwb eng RC109-216 Linlin Song verfasserin aut Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis. Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases Ximeng Han verfasserin aut Cunyu Jia verfasserin aut Xin Zhang verfasserin aut Yunjie Jiao verfasserin aut Taofeng Du verfasserin aut Shuqi Xiao verfasserin aut Julian A. Hiscox verfasserin aut En-Min Zhou verfasserin aut Yang Mu verfasserin aut In Virology Journal BMC, 2004 15(2018), 1, Seite 15 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:15 year:2018 number:1 pages:15 https://doi.org/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 kostenfrei http://link.springer.com/article/10.1186/s12985-018-1081-9 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 15
language	English
source	In Virology Journal 15(2018), 1, Seite 15 volume:15 year:2018 number:1 pages:15
sourceStr	In Virology Journal 15(2018), 1, Seite 15 volume:15 year:2018 number:1 pages:15
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53 Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Virology Journal
authorswithroles_txt_mv	Linlin Song @@aut@@ Ximeng Han @@aut@@ Cunyu Jia @@aut@@ Xin Zhang @@aut@@ Yunjie Jiao @@aut@@ Taofeng Du @@aut@@ Shuqi Xiao @@aut@@ Julian A. Hiscox @@aut@@ En-Min Zhou @@aut@@ Yang Mu @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	394165004
id	DOAJ03900354X
language_de	englisch
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callnumber-first	R - Medicine
author	Linlin Song
spellingShingle	Linlin Song misc RC109-216 misc Porcine reproductive and respiratory syndrome virus misc G2/M arrest misc Cdc2-cyclinB1 complex misc Cdc25C misc p53 misc Infectious and parasitic diseases Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
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topic_title	RC109-216 Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53
topic	misc RC109-216 misc Porcine reproductive and respiratory syndrome virus misc G2/M arrest misc Cdc2-cyclinB1 complex misc Cdc25C misc p53 misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc Porcine reproductive and respiratory syndrome virus misc G2/M arrest misc Cdc2-cyclinB1 complex misc Cdc25C misc p53 misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc Porcine reproductive and respiratory syndrome virus misc G2/M arrest misc Cdc2-cyclinB1 complex misc Cdc25C misc p53 misc Infectious and parasitic diseases
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title	Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
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title_full	Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
author_sort	Linlin Song
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author_browse	Linlin Song Ximeng Han Cunyu Jia Xin Zhang Yunjie Jiao Taofeng Du Shuqi Xiao Julian A. Hiscox En-Min Zhou Yang Mu
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author-letter	Linlin Song
doi_str_mv	10.1186/s12985-018-1081-9
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title_sort	porcine reproductive and respiratory syndrome virus inhibits marc-145 proliferation via inducing apoptosis and g2/m arrest by activation of chk/cdc25c and p53/p21 pathway
callnumber	RC109-216
title_auth	Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
abstract	Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis.
abstractGer	Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis.
abstract_unstemmed	Abstract Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14–3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis.
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title_short	Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
url	https://doi.org/10.1186/s12985-018-1081-9 https://doaj.org/article/f1ff5f2f007f463db974bff1aaff80c1 http://link.springer.com/article/10.1186/s12985-018-1081-9 https://doaj.org/toc/1743-422X
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author2	Ximeng Han Cunyu Jia Xin Zhang Yunjie Jiao Taofeng Du Shuqi Xiao Julian A. Hiscox En-Min Zhou Yang Mu
author2Str	Ximeng Han Cunyu Jia Xin Zhang Yunjie Jiao Taofeng Du Shuqi Xiao Julian A. Hiscox En-Min Zhou Yang Mu
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up_date	2024-07-03T21:05:08.666Z
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Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

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