Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat

Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Baci...
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Autor*in:	Muhammad Zubair [verfasserIn] Alvina Hanif [verfasserIn] Ayaz Farzand [verfasserIn] Taha Majid Mahmood Sheikh [verfasserIn] Abdur Rashid Khan [verfasserIn] Muhammad Suleman [verfasserIn] Muhammad Ayaz [verfasserIn] Xuewen Gao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	psychrophilic PGPR genetic screening signal transduction osmotic regulation plant-stress response

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 7(2019), 9, p 337
Übergeordnetes Werk:	volume:7 ; year:2019 ; number:9, p 337

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms7090337

Katalog-ID:	DOAJ039358356

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authorswithroles_txt_mv	Muhammad Zubair @@aut@@ Alvina Hanif @@aut@@ Ayaz Farzand @@aut@@ Taha Majid Mahmood Sheikh @@aut@@ Abdur Rashid Khan @@aut@@ Muhammad Suleman @@aut@@ Muhammad Ayaz @@aut@@ Xuewen Gao @@aut@@
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callnumber-first	Q - Science
author	Muhammad Zubair
spellingShingle	Muhammad Zubair misc QH301-705.5 misc psychrophilic misc PGPR misc genetic screening misc signal transduction misc osmotic regulation misc plant-stress response misc Biology (General) Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
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topic_title	QH301-705.5 Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat psychrophilic PGPR genetic screening signal transduction osmotic regulation plant-stress response
topic	misc QH301-705.5 misc psychrophilic misc PGPR misc genetic screening misc signal transduction misc osmotic regulation misc plant-stress response misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc psychrophilic misc PGPR misc genetic screening misc signal transduction misc osmotic regulation misc plant-stress response misc Biology (General)
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title	Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
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title_full	Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
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author_browse	Muhammad Zubair Alvina Hanif Ayaz Farzand Taha Majid Mahmood Sheikh Abdur Rashid Khan Muhammad Suleman Muhammad Ayaz Xuewen Gao
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title_sort	genetic screening and expression analysis of psychrophilic <i<bacillus</i< spp. reveal their potential to alleviate cold stress and modulate phytohormones in wheat
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title_auth	Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
abstract	Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. The genetic screening of psychrophilic <i<Bacillus</i< spp. CJCL2, RJGP41 and temperate <i<B. velezensis</i< FZB42 revealed presence of genetic features corresponding to cold stress response, membrane transport, signal transduction and osmotic regulation. Subsequently, the time frame study for the expression of genes involved in these pathways was also significantly higher in psychrophilic strains as analyzed through qPCR analysis at 4 ℃. The inoculated cold tolerant Bacillus strains also aided in inducing stress response in wheat by regulating abscisic acid, lipid peroxidation and proline accumulation pathways in a beneficial manner. Moreover, during comparative analysis of growth promotion in wheat all three Bacillus strains showed significant results at 25 ℃. Whereas, psychrophilic Bacillus strains CJCL2 and RJGP41 were able to positively regulate the expression of phytohormones leading to significant improvement in plant growth under cold stress.
abstractGer	Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. The genetic screening of psychrophilic <i<Bacillus</i< spp. CJCL2, RJGP41 and temperate <i<B. velezensis</i< FZB42 revealed presence of genetic features corresponding to cold stress response, membrane transport, signal transduction and osmotic regulation. Subsequently, the time frame study for the expression of genes involved in these pathways was also significantly higher in psychrophilic strains as analyzed through qPCR analysis at 4 ℃. The inoculated cold tolerant Bacillus strains also aided in inducing stress response in wheat by regulating abscisic acid, lipid peroxidation and proline accumulation pathways in a beneficial manner. Moreover, during comparative analysis of growth promotion in wheat all three Bacillus strains showed significant results at 25 ℃. Whereas, psychrophilic Bacillus strains CJCL2 and RJGP41 were able to positively regulate the expression of phytohormones leading to significant improvement in plant growth under cold stress.
abstract_unstemmed	Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. The genetic screening of psychrophilic <i<Bacillus</i< spp. CJCL2, RJGP41 and temperate <i<B. velezensis</i< FZB42 revealed presence of genetic features corresponding to cold stress response, membrane transport, signal transduction and osmotic regulation. Subsequently, the time frame study for the expression of genes involved in these pathways was also significantly higher in psychrophilic strains as analyzed through qPCR analysis at 4 ℃. The inoculated cold tolerant Bacillus strains also aided in inducing stress response in wheat by regulating abscisic acid, lipid peroxidation and proline accumulation pathways in a beneficial manner. Moreover, during comparative analysis of growth promotion in wheat all three Bacillus strains showed significant results at 25 ℃. Whereas, psychrophilic Bacillus strains CJCL2 and RJGP41 were able to positively regulate the expression of phytohormones leading to significant improvement in plant growth under cold stress.
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title_short	Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
url	https://doi.org/10.3390/microorganisms7090337 https://doaj.org/article/b5a0d91e591c4c5b9e4cac1d9c6b06e9 https://www.mdpi.com/2076-2607/7/9/337 https://doaj.org/toc/2076-2607
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up_date	2024-07-03T22:57:51.414Z
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Genetic Screening and Expression Analysis of Psychrophilic <i<Bacillus</i< spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat

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