Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction

The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficien...
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Autor*in:	Sanjar Batirovich Madrakhimov [verfasserIn] Jin Young Yang [verfasserIn] Dong Hyuck Ahn [verfasserIn] Jung Woo Han [verfasserIn] Tae Ho Ha [verfasserIn] Tae Kwann Park [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system

Übergeordnetes Werk:	In: Molecular Therapy: Methods & Clinical Development - Elsevier, 2015, 17(2020), Seite 647-656
Übergeordnetes Werk:	volume:17 ; year:2020 ; pages:647-656

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.omtm.2020.03.018

Katalog-ID:	DOAJ039664260

Internformat


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520			\|a The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials.
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700	0		\|a Tae Kwann Park \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4393
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951			\|a AR
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publishDate	2020
allfields	10.1016/j.omtm.2020.03.018 doi (DE-627)DOAJ039664260 (DE-599)DOAJcb5e013960004503a1f2d81215e619f9 DE-627 ger DE-627 rakwb eng QH426-470 QH573-671 Sanjar Batirovich Madrakhimov verfasserin aut Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials. Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology Jin Young Yang verfasserin aut Dong Hyuck Ahn verfasserin aut Jung Woo Han verfasserin aut Tae Ho Ha verfasserin aut Tae Kwann Park verfasserin aut In Molecular Therapy: Methods & Clinical Development Elsevier, 2015 17(2020), Seite 647-656 (DE-627)863823203 (DE-600)2863173-0 23290501 nnns volume:17 year:2020 pages:647-656 https://doi.org/10.1016/j.omtm.2020.03.018 kostenfrei https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2329050120300486 kostenfrei https://doaj.org/toc/2329-0501 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 17 2020 647-656
spelling	10.1016/j.omtm.2020.03.018 doi (DE-627)DOAJ039664260 (DE-599)DOAJcb5e013960004503a1f2d81215e619f9 DE-627 ger DE-627 rakwb eng QH426-470 QH573-671 Sanjar Batirovich Madrakhimov verfasserin aut Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials. Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology Jin Young Yang verfasserin aut Dong Hyuck Ahn verfasserin aut Jung Woo Han verfasserin aut Tae Ho Ha verfasserin aut Tae Kwann Park verfasserin aut In Molecular Therapy: Methods & Clinical Development Elsevier, 2015 17(2020), Seite 647-656 (DE-627)863823203 (DE-600)2863173-0 23290501 nnns volume:17 year:2020 pages:647-656 https://doi.org/10.1016/j.omtm.2020.03.018 kostenfrei https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2329050120300486 kostenfrei https://doaj.org/toc/2329-0501 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 17 2020 647-656
allfields_unstemmed	10.1016/j.omtm.2020.03.018 doi (DE-627)DOAJ039664260 (DE-599)DOAJcb5e013960004503a1f2d81215e619f9 DE-627 ger DE-627 rakwb eng QH426-470 QH573-671 Sanjar Batirovich Madrakhimov verfasserin aut Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials. Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology Jin Young Yang verfasserin aut Dong Hyuck Ahn verfasserin aut Jung Woo Han verfasserin aut Tae Ho Ha verfasserin aut Tae Kwann Park verfasserin aut In Molecular Therapy: Methods & Clinical Development Elsevier, 2015 17(2020), Seite 647-656 (DE-627)863823203 (DE-600)2863173-0 23290501 nnns volume:17 year:2020 pages:647-656 https://doi.org/10.1016/j.omtm.2020.03.018 kostenfrei https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2329050120300486 kostenfrei https://doaj.org/toc/2329-0501 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 17 2020 647-656
allfieldsGer	10.1016/j.omtm.2020.03.018 doi (DE-627)DOAJ039664260 (DE-599)DOAJcb5e013960004503a1f2d81215e619f9 DE-627 ger DE-627 rakwb eng QH426-470 QH573-671 Sanjar Batirovich Madrakhimov verfasserin aut Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials. Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology Jin Young Yang verfasserin aut Dong Hyuck Ahn verfasserin aut Jung Woo Han verfasserin aut Tae Ho Ha verfasserin aut Tae Kwann Park verfasserin aut In Molecular Therapy: Methods & Clinical Development Elsevier, 2015 17(2020), Seite 647-656 (DE-627)863823203 (DE-600)2863173-0 23290501 nnns volume:17 year:2020 pages:647-656 https://doi.org/10.1016/j.omtm.2020.03.018 kostenfrei https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2329050120300486 kostenfrei https://doaj.org/toc/2329-0501 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 17 2020 647-656
allfieldsSound	10.1016/j.omtm.2020.03.018 doi (DE-627)DOAJ039664260 (DE-599)DOAJcb5e013960004503a1f2d81215e619f9 DE-627 ger DE-627 rakwb eng QH426-470 QH573-671 Sanjar Batirovich Madrakhimov verfasserin aut Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials. Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology Jin Young Yang verfasserin aut Dong Hyuck Ahn verfasserin aut Jung Woo Han verfasserin aut Tae Ho Ha verfasserin aut Tae Kwann Park verfasserin aut In Molecular Therapy: Methods & Clinical Development Elsevier, 2015 17(2020), Seite 647-656 (DE-627)863823203 (DE-600)2863173-0 23290501 nnns volume:17 year:2020 pages:647-656 https://doi.org/10.1016/j.omtm.2020.03.018 kostenfrei https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2329050120300486 kostenfrei https://doaj.org/toc/2329-0501 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 17 2020 647-656
language	English
source	In Molecular Therapy: Methods & Clinical Development 17(2020), Seite 647-656 volume:17 year:2020 pages:647-656
sourceStr	In Molecular Therapy: Methods & Clinical Development 17(2020), Seite 647-656 volume:17 year:2020 pages:647-656
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system Genetics Cytology
isfreeaccess_bool	true
container_title	Molecular Therapy: Methods & Clinical Development
authorswithroles_txt_mv	Sanjar Batirovich Madrakhimov @@aut@@ Jin Young Yang @@aut@@ Dong Hyuck Ahn @@aut@@ Jung Woo Han @@aut@@ Tae Ho Ha @@aut@@ Tae Kwann Park @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	863823203
id	DOAJ039664260
language_de	englisch
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callnumber-first	Q - Science
author	Sanjar Batirovich Madrakhimov
spellingShingle	Sanjar Batirovich Madrakhimov misc QH426-470 misc QH573-671 misc Adeno-associated viral vector misc gene therapy misc peripapillary intravitreal injection misc intravitreal injection system misc Genetics misc Cytology Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
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topic_title	QH426-470 QH573-671 Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction Adeno-associated viral vector gene therapy peripapillary intravitreal injection intravitreal injection system
topic	misc QH426-470 misc QH573-671 misc Adeno-associated viral vector misc gene therapy misc peripapillary intravitreal injection misc intravitreal injection system misc Genetics misc Cytology
topic_unstemmed	misc QH426-470 misc QH573-671 misc Adeno-associated viral vector misc gene therapy misc peripapillary intravitreal injection misc intravitreal injection system misc Genetics misc Cytology
topic_browse	misc QH426-470 misc QH573-671 misc Adeno-associated viral vector misc gene therapy misc peripapillary intravitreal injection misc intravitreal injection system misc Genetics misc Cytology
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hierarchy_parent_title	Molecular Therapy: Methods & Clinical Development
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title	Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
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title_full	Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
author_sort	Sanjar Batirovich Madrakhimov
journal	Molecular Therapy: Methods & Clinical Development
journalStr	Molecular Therapy: Methods & Clinical Development
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author_browse	Sanjar Batirovich Madrakhimov Jin Young Yang Dong Hyuck Ahn Jung Woo Han Tae Ho Ha Tae Kwann Park
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doi_str_mv	10.1016/j.omtm.2020.03.018
author2-role	verfasserin
title_sort	peripapillary intravitreal injection improves aav-mediated retinal transduction
callnumber	QH426-470
title_auth	Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
abstract	The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials.
abstractGer	The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials.
abstract_unstemmed	The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except for transient, mild hyperemia. In the tissues of the rabbits in the peripapillary injection group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers (p < 0.01). There were no differences between groups in glial activation and expressions of interleukin (IL)-6 and IL-8. These results suggest that peripapillary IVT injection in front of the retina would be safe and efficiently transduce viral vectors into the retina of large animals and is considered as a potential method for use in clinical trials.
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title_short	Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
url	https://doi.org/10.1016/j.omtm.2020.03.018 https://doaj.org/article/cb5e013960004503a1f2d81215e619f9 http://www.sciencedirect.com/science/article/pii/S2329050120300486 https://doaj.org/toc/2329-0501
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up_date	2024-07-04T00:16:05.619Z
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