The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study

Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospe...
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Autor*in:	Hartuis Sophie [verfasserIn] Lavergne Rose-Anne [verfasserIn] Nourrisson Céline [verfasserIn] Verweij Jaco [verfasserIn] Desoubeaux Guillaume [verfasserIn] Lussac-Sorton Florian [verfasserIn] Lemoine Jean-Philippe [verfasserIn] Cateau Estelle [verfasserIn] Jeddi Fakhri [verfasserIn] Poirier Philippe [verfasserIn] Le Pape Patrice [verfasserIn] Morio Florent [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation

Übergeordnetes Werk:	In: Parasite - EDP Sciences, 2015, 29, p 27(2022)
Übergeordnetes Werk:	volume:29, p 27 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1051/parasite/2022026

Katalog-ID:	DOAJ03974888X

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allfields	10.1051/parasite/2022026 doi (DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c DE-627 ger DE-627 rakwb eng RC109-216 Hartuis Sophie verfasserin aut The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools. stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases Lavergne Rose-Anne verfasserin aut Nourrisson Céline verfasserin aut Verweij Jaco verfasserin aut Desoubeaux Guillaume verfasserin aut Lussac-Sorton Florian verfasserin aut Lemoine Jean-Philippe verfasserin aut Cateau Estelle verfasserin aut Jeddi Fakhri verfasserin aut Poirier Philippe verfasserin aut Le Pape Patrice verfasserin aut Morio Florent verfasserin aut In Parasite EDP Sciences, 2015 29, p 27(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 27 year:2022 https://doi.org/10.1051/parasite/2022026 kostenfrei https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 27 2022
spelling	10.1051/parasite/2022026 doi (DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c DE-627 ger DE-627 rakwb eng RC109-216 Hartuis Sophie verfasserin aut The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools. stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases Lavergne Rose-Anne verfasserin aut Nourrisson Céline verfasserin aut Verweij Jaco verfasserin aut Desoubeaux Guillaume verfasserin aut Lussac-Sorton Florian verfasserin aut Lemoine Jean-Philippe verfasserin aut Cateau Estelle verfasserin aut Jeddi Fakhri verfasserin aut Poirier Philippe verfasserin aut Le Pape Patrice verfasserin aut Morio Florent verfasserin aut In Parasite EDP Sciences, 2015 29, p 27(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 27 year:2022 https://doi.org/10.1051/parasite/2022026 kostenfrei https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 27 2022
allfields_unstemmed	10.1051/parasite/2022026 doi (DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c DE-627 ger DE-627 rakwb eng RC109-216 Hartuis Sophie verfasserin aut The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools. stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases Lavergne Rose-Anne verfasserin aut Nourrisson Céline verfasserin aut Verweij Jaco verfasserin aut Desoubeaux Guillaume verfasserin aut Lussac-Sorton Florian verfasserin aut Lemoine Jean-Philippe verfasserin aut Cateau Estelle verfasserin aut Jeddi Fakhri verfasserin aut Poirier Philippe verfasserin aut Le Pape Patrice verfasserin aut Morio Florent verfasserin aut In Parasite EDP Sciences, 2015 29, p 27(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 27 year:2022 https://doi.org/10.1051/parasite/2022026 kostenfrei https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 27 2022
allfieldsGer	10.1051/parasite/2022026 doi (DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c DE-627 ger DE-627 rakwb eng RC109-216 Hartuis Sophie verfasserin aut The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools. stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases Lavergne Rose-Anne verfasserin aut Nourrisson Céline verfasserin aut Verweij Jaco verfasserin aut Desoubeaux Guillaume verfasserin aut Lussac-Sorton Florian verfasserin aut Lemoine Jean-Philippe verfasserin aut Cateau Estelle verfasserin aut Jeddi Fakhri verfasserin aut Poirier Philippe verfasserin aut Le Pape Patrice verfasserin aut Morio Florent verfasserin aut In Parasite EDP Sciences, 2015 29, p 27(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 27 year:2022 https://doi.org/10.1051/parasite/2022026 kostenfrei https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 27 2022
allfieldsSound	10.1051/parasite/2022026 doi (DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c DE-627 ger DE-627 rakwb eng RC109-216 Hartuis Sophie verfasserin aut The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools. stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases Lavergne Rose-Anne verfasserin aut Nourrisson Céline verfasserin aut Verweij Jaco verfasserin aut Desoubeaux Guillaume verfasserin aut Lussac-Sorton Florian verfasserin aut Lemoine Jean-Philippe verfasserin aut Cateau Estelle verfasserin aut Jeddi Fakhri verfasserin aut Poirier Philippe verfasserin aut Le Pape Patrice verfasserin aut Morio Florent verfasserin aut In Parasite EDP Sciences, 2015 29, p 27(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 27 year:2022 https://doi.org/10.1051/parasite/2022026 kostenfrei https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 27 2022
language	English
source	In Parasite 29, p 27(2022) volume:29, p 27 year:2022
sourceStr	In Parasite 29, p 27(2022) volume:29, p 27 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Parasite
authorswithroles_txt_mv	Hartuis Sophie @@aut@@ Lavergne Rose-Anne @@aut@@ Nourrisson Céline @@aut@@ Verweij Jaco @@aut@@ Desoubeaux Guillaume @@aut@@ Lussac-Sorton Florian @@aut@@ Lemoine Jean-Philippe @@aut@@ Cateau Estelle @@aut@@ Jeddi Fakhri @@aut@@ Poirier Philippe @@aut@@ Le Pape Patrice @@aut@@ Morio Florent @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	527642398
id	DOAJ03974888X
language_de	englisch
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callnumber-first	R - Medicine
author	Hartuis Sophie
spellingShingle	Hartuis Sophie misc RC109-216 misc stool misc gastrointestinal parasites misc microscopy misc novodiag® stool parasites misc high-plex detection misc prospective study misc comparative evaluation misc Infectious and parasitic diseases The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
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topic_title	RC109-216 The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study stool gastrointestinal parasites microscopy novodiag® stool parasites high-plex detection prospective study comparative evaluation
topic	misc RC109-216 misc stool misc gastrointestinal parasites misc microscopy misc novodiag® stool parasites misc high-plex detection misc prospective study misc comparative evaluation misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc stool misc gastrointestinal parasites misc microscopy misc novodiag® stool parasites misc high-plex detection misc prospective study misc comparative evaluation misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc stool misc gastrointestinal parasites misc microscopy misc novodiag® stool parasites misc high-plex detection misc prospective study misc comparative evaluation misc Infectious and parasitic diseases
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title	The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
ctrlnum	(DE-627)DOAJ03974888X (DE-599)DOAJ161b943ecc3b4aef8eb51a105126758c
title_full	The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
author_sort	Hartuis Sophie
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author_browse	Hartuis Sophie Lavergne Rose-Anne Nourrisson Céline Verweij Jaco Desoubeaux Guillaume Lussac-Sorton Florian Lemoine Jean-Philippe Cateau Estelle Jeddi Fakhri Poirier Philippe Le Pape Patrice Morio Florent
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author-letter	Hartuis Sophie
doi_str_mv	10.1051/parasite/2022026
author2-role	verfasserin
title_sort	novodiag® stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
callnumber	RC109-216
title_auth	The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
abstract	Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools.
abstractGer	Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools.
abstract_unstemmed	Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools.
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title_short	The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
url	https://doi.org/10.1051/parasite/2022026 https://doaj.org/article/161b943ecc3b4aef8eb51a105126758c https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210153/parasite210153.html https://doaj.org/toc/1776-1042
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author2	Lavergne Rose-Anne Nourrisson Céline Verweij Jaco Desoubeaux Guillaume Lussac-Sorton Florian Lemoine Jean-Philippe Cateau Estelle Jeddi Fakhri Poirier Philippe Le Pape Patrice Morio Florent
author2Str	Lavergne Rose-Anne Nourrisson Céline Verweij Jaco Desoubeaux Guillaume Lussac-Sorton Florian Lemoine Jean-Philippe Cateau Estelle Jeddi Fakhri Poirier Philippe Le Pape Patrice Morio Florent
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doi_str	10.1051/parasite/2022026
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up_date	2024-07-04T00:35:25.330Z
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The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study

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