Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects

The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in...
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Autor*in:	Camila Benavides-Rivas [verfasserIn] Lina Mariana Tovar [verfasserIn] Nicolás Zúñiga [verfasserIn] Ingrid Pinto-Borguero [verfasserIn] Claudio Retamal [verfasserIn] Gonzalo E. Yévenes [verfasserIn] Gustavo Moraga-Cid [verfasserIn] Jorge Fuentealba [verfasserIn] Leonardo Guzmán [verfasserIn] Claudio Coddou [verfasserIn] Luisa Bascuñán-Godoy [verfasserIn] Patricio A. Castro [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	neurulation neural tube defects glutamate glutaminase 1 DON

Übergeordnetes Werk:	In: Frontiers in Pharmacology - Frontiers Media S.A., 2010, 11(2020)
Übergeordnetes Werk:	volume:11 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fphar.2020.00900

Katalog-ID:	DOAJ039864820

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spelling	10.3389/fphar.2020.00900 doi (DE-627)DOAJ039864820 (DE-599)DOAJbdb64b2cbc5449f79d0d3705e0d72e68 DE-627 ger DE-627 rakwb eng RM1-950 Camila Benavides-Rivas verfasserin aut Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in this process is glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing the oriented cell migration and proliferation, essentials processes in neurulation. Glutamate synthesis depends on the mitochondrial enzyme known as glutaminase 1 (GLS1) that is widely expressed in brain and kidney. The participation of GLS 1 in prenatal neurogenic processes and in the adult brain has been experimentally established, however, its participation in early stages of embryonic development has not been described. The present investigation describes for the first time the presence and functionality of GLS1 in Xenopus laevis embryos during neurulation. Although protein expression levels remains constant, the catalytic activity of GLS1 increases significantly (~66%) between early (stage 12) and middle to late (stages 14–19) neurulation process. Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos. neurulation neural tube defects glutamate glutaminase 1 DON Therapeutics. Pharmacology Lina Mariana Tovar verfasserin aut Nicolás Zúñiga verfasserin aut Ingrid Pinto-Borguero verfasserin aut Claudio Retamal verfasserin aut Gonzalo E. Yévenes verfasserin aut Gustavo Moraga-Cid verfasserin aut Jorge Fuentealba verfasserin aut Leonardo Guzmán verfasserin aut Claudio Coddou verfasserin aut Luisa Bascuñán-Godoy verfasserin aut Patricio A. Castro verfasserin aut In Frontiers in Pharmacology Frontiers Media S.A., 2010 11(2020) (DE-627)642889392 (DE-600)2587355-6 16639812 nnns volume:11 year:2020 https://doi.org/10.3389/fphar.2020.00900 kostenfrei https://doaj.org/article/bdb64b2cbc5449f79d0d3705e0d72e68 kostenfrei https://www.frontiersin.org/article/10.3389/fphar.2020.00900/full kostenfrei https://doaj.org/toc/1663-9812 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
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allfieldsSound	10.3389/fphar.2020.00900 doi (DE-627)DOAJ039864820 (DE-599)DOAJbdb64b2cbc5449f79d0d3705e0d72e68 DE-627 ger DE-627 rakwb eng RM1-950 Camila Benavides-Rivas verfasserin aut Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in this process is glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing the oriented cell migration and proliferation, essentials processes in neurulation. Glutamate synthesis depends on the mitochondrial enzyme known as glutaminase 1 (GLS1) that is widely expressed in brain and kidney. The participation of GLS 1 in prenatal neurogenic processes and in the adult brain has been experimentally established, however, its participation in early stages of embryonic development has not been described. The present investigation describes for the first time the presence and functionality of GLS1 in Xenopus laevis embryos during neurulation. Although protein expression levels remains constant, the catalytic activity of GLS1 increases significantly (~66%) between early (stage 12) and middle to late (stages 14–19) neurulation process. Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos. neurulation neural tube defects glutamate glutaminase 1 DON Therapeutics. Pharmacology Lina Mariana Tovar verfasserin aut Nicolás Zúñiga verfasserin aut Ingrid Pinto-Borguero verfasserin aut Claudio Retamal verfasserin aut Gonzalo E. Yévenes verfasserin aut Gustavo Moraga-Cid verfasserin aut Jorge Fuentealba verfasserin aut Leonardo Guzmán verfasserin aut Claudio Coddou verfasserin aut Luisa Bascuñán-Godoy verfasserin aut Patricio A. Castro verfasserin aut In Frontiers in Pharmacology Frontiers Media S.A., 2010 11(2020) (DE-627)642889392 (DE-600)2587355-6 16639812 nnns volume:11 year:2020 https://doi.org/10.3389/fphar.2020.00900 kostenfrei https://doaj.org/article/bdb64b2cbc5449f79d0d3705e0d72e68 kostenfrei https://www.frontiersin.org/article/10.3389/fphar.2020.00900/full kostenfrei https://doaj.org/toc/1663-9812 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
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callnumber-first	R - Medicine
author	Camila Benavides-Rivas
spellingShingle	Camila Benavides-Rivas misc RM1-950 misc neurulation misc neural tube defects misc glutamate misc glutaminase 1 misc DON misc Therapeutics. Pharmacology Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects
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topic_title	RM1-950 Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects neurulation neural tube defects glutamate glutaminase 1 DON
topic	misc RM1-950 misc neurulation misc neural tube defects misc glutamate misc glutaminase 1 misc DON misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc neurulation misc neural tube defects misc glutamate misc glutaminase 1 misc DON misc Therapeutics. Pharmacology
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title	Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects
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title_sort	altered glutaminase 1 activity during neurulation and its potential implications in neural tube defects
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title_auth	Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects
abstract	The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in this process is glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing the oriented cell migration and proliferation, essentials processes in neurulation. Glutamate synthesis depends on the mitochondrial enzyme known as glutaminase 1 (GLS1) that is widely expressed in brain and kidney. The participation of GLS 1 in prenatal neurogenic processes and in the adult brain has been experimentally established, however, its participation in early stages of embryonic development has not been described. The present investigation describes for the first time the presence and functionality of GLS1 in Xenopus laevis embryos during neurulation. Although protein expression levels remains constant, the catalytic activity of GLS1 increases significantly (~66%) between early (stage 12) and middle to late (stages 14–19) neurulation process. Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos.
abstractGer	The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in this process is glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing the oriented cell migration and proliferation, essentials processes in neurulation. Glutamate synthesis depends on the mitochondrial enzyme known as glutaminase 1 (GLS1) that is widely expressed in brain and kidney. The participation of GLS 1 in prenatal neurogenic processes and in the adult brain has been experimentally established, however, its participation in early stages of embryonic development has not been described. The present investigation describes for the first time the presence and functionality of GLS1 in Xenopus laevis embryos during neurulation. Although protein expression levels remains constant, the catalytic activity of GLS1 increases significantly (~66%) between early (stage 12) and middle to late (stages 14–19) neurulation process. Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos.
abstract_unstemmed	The neurulation process is regulated by a large amount of genetic and environmental factors that determine the establishment, folding, and fusion of the neural plate to form the neural tube, which develops into the main structure of the central nervous system. A recently described factor involved in this process is glutamate. Through NMDA ionotropic receptor, glutamate modifies intracellular Ca2+ dynamics allowing the oriented cell migration and proliferation, essentials processes in neurulation. Glutamate synthesis depends on the mitochondrial enzyme known as glutaminase 1 (GLS1) that is widely expressed in brain and kidney. The participation of GLS 1 in prenatal neurogenic processes and in the adult brain has been experimentally established, however, its participation in early stages of embryonic development has not been described. The present investigation describes for the first time the presence and functionality of GLS1 in Xenopus laevis embryos during neurulation. Although protein expression levels remains constant, the catalytic activity of GLS1 increases significantly (~66%) between early (stage 12) and middle to late (stages 14–19) neurulation process. Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos.
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title_short	Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects
url	https://doi.org/10.3389/fphar.2020.00900 https://doaj.org/article/bdb64b2cbc5449f79d0d3705e0d72e68 https://www.frontiersin.org/article/10.3389/fphar.2020.00900/full https://doaj.org/toc/1663-9812
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author2	Lina Mariana Tovar Nicolás Zúñiga Ingrid Pinto-Borguero Claudio Retamal Gonzalo E. Yévenes Gustavo Moraga-Cid Jorge Fuentealba Leonardo Guzmán Claudio Coddou Luisa Bascuñán-Godoy Patricio A. Castro
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Additionally, the use of 6-diazo-5-oxo-L-norleucine (L-DON, competitive inhibitor of glutamine-depend enzymes), reduced significantly the GLS1 specific activity during neurulation (~36%) and induce the occurrence of neural tube defects involving its possible participation in the neural tube closure in Xenopus laevis embryos.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">neurulation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">neural tube defects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">glutamate</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">glutaminase 1</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DON</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Therapeutics. 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Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects

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