Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis?
Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study dete...
Ausführliche Beschreibung
Autor*in: |
Yoo Jin Lee [verfasserIn] Seojin Kim [verfasserIn] Youngjin Kang [verfasserIn] Jiyoon Jung [verfasserIn] Eunjung Lee [verfasserIn] Joo-Young Kim [verfasserIn] Jeong Hyeon Lee [verfasserIn] Youngseok Lee [verfasserIn] Yang-seok Chae [verfasserIn] Chul Hwan Kim [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch ; Koreanisch |
Erschienen: |
2016 |
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Übergeordnetes Werk: |
In: Journal of Pathology and Translational Medicine - Korean Society of Pathologists & the Korean Society for Cytopathology, 2017, 50(2016), 6, Seite 451-458 |
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Übergeordnetes Werk: |
volume:50 ; year:2016 ; number:6 ; pages:451-458 |
Links: |
Link aufrufen |
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DOI / URN: |
10.4132/jptm.2016.08.04 |
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Katalog-ID: |
DOAJ040140830 |
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520 | |a Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. | ||
650 | 4 | |a Tuberculosis | |
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650 | 4 | |a Interferon-γ release tests | |
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700 | 0 | |a Chul Hwan Kim |e verfasserin |4 aut | |
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10.4132/jptm.2016.08.04 doi (DE-627)DOAJ040140830 (DE-599)DOAJ07e66ee91d9d45099a401df8c3808011 DE-627 ger DE-627 rakwb eng kor RB1-214 Yoo Jin Lee verfasserin aut Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. Tuberculosis Polymerase chain reaction Mycobacterial culture Interferon-γ release tests Pathology Seojin Kim verfasserin aut Youngjin Kang verfasserin aut Jiyoon Jung verfasserin aut Eunjung Lee verfasserin aut Joo-Young Kim verfasserin aut Jeong Hyeon Lee verfasserin aut Youngseok Lee verfasserin aut Yang-seok Chae verfasserin aut Chul Hwan Kim verfasserin aut In Journal of Pathology and Translational Medicine Korean Society of Pathologists & the Korean Society for Cytopathology, 2017 50(2016), 6, Seite 451-458 (DE-627)169835603X 23837845 nnns volume:50 year:2016 number:6 pages:451-458 https://doi.org/10.4132/jptm.2016.08.04 kostenfrei https://doaj.org/article/07e66ee91d9d45099a401df8c3808011 kostenfrei http://www.jpatholtm.org/upload/pdf/jptm-2016-08-04.pdf kostenfrei https://doaj.org/toc/2383-7837 Journal toc kostenfrei https://doaj.org/toc/2383-7845 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 50 2016 6 451-458 |
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10.4132/jptm.2016.08.04 doi (DE-627)DOAJ040140830 (DE-599)DOAJ07e66ee91d9d45099a401df8c3808011 DE-627 ger DE-627 rakwb eng kor RB1-214 Yoo Jin Lee verfasserin aut Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. Tuberculosis Polymerase chain reaction Mycobacterial culture Interferon-γ release tests Pathology Seojin Kim verfasserin aut Youngjin Kang verfasserin aut Jiyoon Jung verfasserin aut Eunjung Lee verfasserin aut Joo-Young Kim verfasserin aut Jeong Hyeon Lee verfasserin aut Youngseok Lee verfasserin aut Yang-seok Chae verfasserin aut Chul Hwan Kim verfasserin aut In Journal of Pathology and Translational Medicine Korean Society of Pathologists & the Korean Society for Cytopathology, 2017 50(2016), 6, Seite 451-458 (DE-627)169835603X 23837845 nnns volume:50 year:2016 number:6 pages:451-458 https://doi.org/10.4132/jptm.2016.08.04 kostenfrei https://doaj.org/article/07e66ee91d9d45099a401df8c3808011 kostenfrei http://www.jpatholtm.org/upload/pdf/jptm-2016-08-04.pdf kostenfrei https://doaj.org/toc/2383-7837 Journal toc kostenfrei https://doaj.org/toc/2383-7845 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 50 2016 6 451-458 |
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10.4132/jptm.2016.08.04 doi (DE-627)DOAJ040140830 (DE-599)DOAJ07e66ee91d9d45099a401df8c3808011 DE-627 ger DE-627 rakwb eng kor RB1-214 Yoo Jin Lee verfasserin aut Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. Tuberculosis Polymerase chain reaction Mycobacterial culture Interferon-γ release tests Pathology Seojin Kim verfasserin aut Youngjin Kang verfasserin aut Jiyoon Jung verfasserin aut Eunjung Lee verfasserin aut Joo-Young Kim verfasserin aut Jeong Hyeon Lee verfasserin aut Youngseok Lee verfasserin aut Yang-seok Chae verfasserin aut Chul Hwan Kim verfasserin aut In Journal of Pathology and Translational Medicine Korean Society of Pathologists & the Korean Society for Cytopathology, 2017 50(2016), 6, Seite 451-458 (DE-627)169835603X 23837845 nnns volume:50 year:2016 number:6 pages:451-458 https://doi.org/10.4132/jptm.2016.08.04 kostenfrei https://doaj.org/article/07e66ee91d9d45099a401df8c3808011 kostenfrei http://www.jpatholtm.org/upload/pdf/jptm-2016-08-04.pdf kostenfrei https://doaj.org/toc/2383-7837 Journal toc kostenfrei https://doaj.org/toc/2383-7845 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 50 2016 6 451-458 |
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10.4132/jptm.2016.08.04 doi (DE-627)DOAJ040140830 (DE-599)DOAJ07e66ee91d9d45099a401df8c3808011 DE-627 ger DE-627 rakwb eng kor RB1-214 Yoo Jin Lee verfasserin aut Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. Tuberculosis Polymerase chain reaction Mycobacterial culture Interferon-γ release tests Pathology Seojin Kim verfasserin aut Youngjin Kang verfasserin aut Jiyoon Jung verfasserin aut Eunjung Lee verfasserin aut Joo-Young Kim verfasserin aut Jeong Hyeon Lee verfasserin aut Youngseok Lee verfasserin aut Yang-seok Chae verfasserin aut Chul Hwan Kim verfasserin aut In Journal of Pathology and Translational Medicine Korean Society of Pathologists & the Korean Society for Cytopathology, 2017 50(2016), 6, Seite 451-458 (DE-627)169835603X 23837845 nnns volume:50 year:2016 number:6 pages:451-458 https://doi.org/10.4132/jptm.2016.08.04 kostenfrei https://doaj.org/article/07e66ee91d9d45099a401df8c3808011 kostenfrei http://www.jpatholtm.org/upload/pdf/jptm-2016-08-04.pdf kostenfrei https://doaj.org/toc/2383-7837 Journal toc kostenfrei https://doaj.org/toc/2383-7845 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 50 2016 6 451-458 |
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10.4132/jptm.2016.08.04 doi (DE-627)DOAJ040140830 (DE-599)DOAJ07e66ee91d9d45099a401df8c3808011 DE-627 ger DE-627 rakwb eng kor RB1-214 Yoo Jin Lee verfasserin aut Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. Tuberculosis Polymerase chain reaction Mycobacterial culture Interferon-γ release tests Pathology Seojin Kim verfasserin aut Youngjin Kang verfasserin aut Jiyoon Jung verfasserin aut Eunjung Lee verfasserin aut Joo-Young Kim verfasserin aut Jeong Hyeon Lee verfasserin aut Youngseok Lee verfasserin aut Yang-seok Chae verfasserin aut Chul Hwan Kim verfasserin aut In Journal of Pathology and Translational Medicine Korean Society of Pathologists & the Korean Society for Cytopathology, 2017 50(2016), 6, Seite 451-458 (DE-627)169835603X 23837845 nnns volume:50 year:2016 number:6 pages:451-458 https://doi.org/10.4132/jptm.2016.08.04 kostenfrei https://doaj.org/article/07e66ee91d9d45099a401df8c3808011 kostenfrei http://www.jpatholtm.org/upload/pdf/jptm-2016-08-04.pdf kostenfrei https://doaj.org/toc/2383-7837 Journal toc kostenfrei https://doaj.org/toc/2383-7845 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 50 2016 6 451-458 |
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Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis? |
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Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. |
abstractGer |
Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. |
abstract_unstemmed |
Background Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens. Methods We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated. Results The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. |
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The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features. Conclusions PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. 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