A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta)

As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jiao Yin [verfasserIn] Aiming Lu [verfasserIn] Tuanjie Che [verfasserIn] Lihong He [verfasserIn] Songdong Shen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	water temperature growth cyclin extracellular signal-regulated kinase

Übergeordnetes Werk:	In: Water - MDPI AG, 2010, 14(2022), 14, p 2175
Übergeordnetes Werk:	volume:14 ; year:2022 ; number:14, p 2175

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/w14142175

Katalog-ID:	DOAJ040197581

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520			\|a As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures.
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allfields	10.3390/w14142175 doi (DE-627)DOAJ040197581 (DE-599)DOAJbc5c593ed2fa42a7b98a79210be52270 DE-627 ger DE-627 rakwb eng TC1-978 TD201-500 Jiao Yin verfasserin aut A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. <i<Neopyropia yezoensis</i< water temperature growth cyclin extracellular signal-regulated kinase Hydraulic engineering Water supply for domestic and industrial purposes Aiming Lu verfasserin aut Tuanjie Che verfasserin aut Lihong He verfasserin aut Songdong Shen verfasserin aut In Water MDPI AG, 2010 14(2022), 14, p 2175 (DE-627)611729008 (DE-600)2521238-2 20734441 nnns volume:14 year:2022 number:14, p 2175 https://doi.org/10.3390/w14142175 kostenfrei https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 kostenfrei https://www.mdpi.com/2073-4441/14/14/2175 kostenfrei https://doaj.org/toc/2073-4441 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 14, p 2175
spelling	10.3390/w14142175 doi (DE-627)DOAJ040197581 (DE-599)DOAJbc5c593ed2fa42a7b98a79210be52270 DE-627 ger DE-627 rakwb eng TC1-978 TD201-500 Jiao Yin verfasserin aut A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. <i<Neopyropia yezoensis</i< water temperature growth cyclin extracellular signal-regulated kinase Hydraulic engineering Water supply for domestic and industrial purposes Aiming Lu verfasserin aut Tuanjie Che verfasserin aut Lihong He verfasserin aut Songdong Shen verfasserin aut In Water MDPI AG, 2010 14(2022), 14, p 2175 (DE-627)611729008 (DE-600)2521238-2 20734441 nnns volume:14 year:2022 number:14, p 2175 https://doi.org/10.3390/w14142175 kostenfrei https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 kostenfrei https://www.mdpi.com/2073-4441/14/14/2175 kostenfrei https://doaj.org/toc/2073-4441 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 14, p 2175
allfields_unstemmed	10.3390/w14142175 doi (DE-627)DOAJ040197581 (DE-599)DOAJbc5c593ed2fa42a7b98a79210be52270 DE-627 ger DE-627 rakwb eng TC1-978 TD201-500 Jiao Yin verfasserin aut A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. <i<Neopyropia yezoensis</i< water temperature growth cyclin extracellular signal-regulated kinase Hydraulic engineering Water supply for domestic and industrial purposes Aiming Lu verfasserin aut Tuanjie Che verfasserin aut Lihong He verfasserin aut Songdong Shen verfasserin aut In Water MDPI AG, 2010 14(2022), 14, p 2175 (DE-627)611729008 (DE-600)2521238-2 20734441 nnns volume:14 year:2022 number:14, p 2175 https://doi.org/10.3390/w14142175 kostenfrei https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 kostenfrei https://www.mdpi.com/2073-4441/14/14/2175 kostenfrei https://doaj.org/toc/2073-4441 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 14, p 2175
allfieldsGer	10.3390/w14142175 doi (DE-627)DOAJ040197581 (DE-599)DOAJbc5c593ed2fa42a7b98a79210be52270 DE-627 ger DE-627 rakwb eng TC1-978 TD201-500 Jiao Yin verfasserin aut A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. <i<Neopyropia yezoensis</i< water temperature growth cyclin extracellular signal-regulated kinase Hydraulic engineering Water supply for domestic and industrial purposes Aiming Lu verfasserin aut Tuanjie Che verfasserin aut Lihong He verfasserin aut Songdong Shen verfasserin aut In Water MDPI AG, 2010 14(2022), 14, p 2175 (DE-627)611729008 (DE-600)2521238-2 20734441 nnns volume:14 year:2022 number:14, p 2175 https://doi.org/10.3390/w14142175 kostenfrei https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 kostenfrei https://www.mdpi.com/2073-4441/14/14/2175 kostenfrei https://doaj.org/toc/2073-4441 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 14, p 2175
allfieldsSound	10.3390/w14142175 doi (DE-627)DOAJ040197581 (DE-599)DOAJbc5c593ed2fa42a7b98a79210be52270 DE-627 ger DE-627 rakwb eng TC1-978 TD201-500 Jiao Yin verfasserin aut A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures. <i<Neopyropia yezoensis</i< water temperature growth cyclin extracellular signal-regulated kinase Hydraulic engineering Water supply for domestic and industrial purposes Aiming Lu verfasserin aut Tuanjie Che verfasserin aut Lihong He verfasserin aut Songdong Shen verfasserin aut In Water MDPI AG, 2010 14(2022), 14, p 2175 (DE-627)611729008 (DE-600)2521238-2 20734441 nnns volume:14 year:2022 number:14, p 2175 https://doi.org/10.3390/w14142175 kostenfrei https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 kostenfrei https://www.mdpi.com/2073-4441/14/14/2175 kostenfrei https://doaj.org/toc/2073-4441 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 14, p 2175
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title	A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta)
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title_full	A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta)
author_sort	Jiao Yin
journal	Water
journalStr	Water
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author_browse	Jiao Yin Aiming Lu Tuanjie Che Lihong He Songdong Shen
container_volume	14
class	TC1-978 TD201-500
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author-letter	Jiao Yin
doi_str_mv	10.3390/w14142175
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title_sort	preliminary study on the mechanisms of growth and physiological changes in response to different temperatures in <i<neopyropia yezoensis</i< (rhodophyta)
callnumber	TC1-978
title_auth	A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta)
abstract	As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures.
abstractGer	As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures.
abstract_unstemmed	As an economically valuable red seaweed, <i<Neopyropia yezoensis</i< (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of <i<N. yezoensis.</i< In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in <i<N. yezoensis</i< were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of <i<N. yezoensis</i< at high temperatures.
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container_issue	14, p 2175
title_short	A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in <i<Neopyropia yezoensis</i< (Rhodophyta)
url	https://doi.org/10.3390/w14142175 https://doaj.org/article/bc5c593ed2fa42a7b98a79210be52270 https://www.mdpi.com/2073-4441/14/14/2175 https://doaj.org/toc/2073-4441
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