Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats

<p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Nan-nan ZHANG [verfasserIn] Lin CONG [verfasserIn] Xiu-ying FANG [verfasserIn] Xue-mei FENG [verfasserIn] Xi-ke ZHU [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Chinesisch

Erschienen:	2015

Schlagwörter:	Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal

Übergeordnetes Werk:	In: Chinese Journal of Contemporary Neurology and Neurosurgery - Tianjin Huanhu Hospital, 2013, 15(2015), 11, Seite 890-895
Übergeordnetes Werk:	volume:15 ; year:2015 ; number:11 ; pages:890-895

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Katalog-ID:	DOAJ040270637

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245	1	0	\|a Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
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520			\|a <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p<
650		4	\|a Epilepsy
650		4	\|a Kainic acid
650		4	\|a Diet therapy
650		4	\|a Ketone bodies
650		4	\|a Hippocampus
650		4	\|a Neurons
650		4	\|a Disease models, animal
653		0	\|a Neurology. Diseases of the nervous system
700	0		\|a Lin CONG \|e verfasserin \|4 aut
700	0		\|a Xiu-ying FANG \|e verfasserin \|4 aut
700	0		\|a Xue-mei FENG \|e verfasserin \|4 aut
700	0		\|a Xi-ke ZHU \|e verfasserin \|4 aut
773	0	8	\|i In \|t Chinese Journal of Contemporary Neurology and Neurosurgery \|d Tianjin Huanhu Hospital, 2013 \|g 15(2015), 11, Seite 890-895 \|w (DE-627)741171848 \|w (DE-600)2710763-2 \|x 16726731 \|7 nnns
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allfields	(DE-627)DOAJ040270637 (DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6 DE-627 ger DE-627 rakwb eng chi RC346-429 Nan-nan ZHANG verfasserin aut Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system Lin CONG verfasserin aut Xiu-ying FANG verfasserin aut Xue-mei FENG verfasserin aut Xi-ke ZHU verfasserin aut In Chinese Journal of Contemporary Neurology and Neurosurgery Tianjin Huanhu Hospital, 2013 15(2015), 11, Seite 890-895 (DE-627)741171848 (DE-600)2710763-2 16726731 nnns volume:15 year:2015 number:11 pages:890-895 https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 kostenfrei http://www.cjcnn.org/index.php/cjcnn/article/view/1307 kostenfrei https://doaj.org/toc/1672-6731 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2106 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 11 890-895
spelling	(DE-627)DOAJ040270637 (DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6 DE-627 ger DE-627 rakwb eng chi RC346-429 Nan-nan ZHANG verfasserin aut Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system Lin CONG verfasserin aut Xiu-ying FANG verfasserin aut Xue-mei FENG verfasserin aut Xi-ke ZHU verfasserin aut In Chinese Journal of Contemporary Neurology and Neurosurgery Tianjin Huanhu Hospital, 2013 15(2015), 11, Seite 890-895 (DE-627)741171848 (DE-600)2710763-2 16726731 nnns volume:15 year:2015 number:11 pages:890-895 https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 kostenfrei http://www.cjcnn.org/index.php/cjcnn/article/view/1307 kostenfrei https://doaj.org/toc/1672-6731 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2106 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 11 890-895
allfields_unstemmed	(DE-627)DOAJ040270637 (DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6 DE-627 ger DE-627 rakwb eng chi RC346-429 Nan-nan ZHANG verfasserin aut Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system Lin CONG verfasserin aut Xiu-ying FANG verfasserin aut Xue-mei FENG verfasserin aut Xi-ke ZHU verfasserin aut In Chinese Journal of Contemporary Neurology and Neurosurgery Tianjin Huanhu Hospital, 2013 15(2015), 11, Seite 890-895 (DE-627)741171848 (DE-600)2710763-2 16726731 nnns volume:15 year:2015 number:11 pages:890-895 https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 kostenfrei http://www.cjcnn.org/index.php/cjcnn/article/view/1307 kostenfrei https://doaj.org/toc/1672-6731 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2106 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 11 890-895
allfieldsGer	(DE-627)DOAJ040270637 (DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6 DE-627 ger DE-627 rakwb eng chi RC346-429 Nan-nan ZHANG verfasserin aut Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system Lin CONG verfasserin aut Xiu-ying FANG verfasserin aut Xue-mei FENG verfasserin aut Xi-ke ZHU verfasserin aut In Chinese Journal of Contemporary Neurology and Neurosurgery Tianjin Huanhu Hospital, 2013 15(2015), 11, Seite 890-895 (DE-627)741171848 (DE-600)2710763-2 16726731 nnns volume:15 year:2015 number:11 pages:890-895 https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 kostenfrei http://www.cjcnn.org/index.php/cjcnn/article/view/1307 kostenfrei https://doaj.org/toc/1672-6731 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2106 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 11 890-895
allfieldsSound	(DE-627)DOAJ040270637 (DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6 DE-627 ger DE-627 rakwb eng chi RC346-429 Nan-nan ZHANG verfasserin aut Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p< Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system Lin CONG verfasserin aut Xiu-ying FANG verfasserin aut Xue-mei FENG verfasserin aut Xi-ke ZHU verfasserin aut In Chinese Journal of Contemporary Neurology and Neurosurgery Tianjin Huanhu Hospital, 2013 15(2015), 11, Seite 890-895 (DE-627)741171848 (DE-600)2710763-2 16726731 nnns volume:15 year:2015 number:11 pages:890-895 https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 kostenfrei http://www.cjcnn.org/index.php/cjcnn/article/view/1307 kostenfrei https://doaj.org/toc/1672-6731 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2106 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 11 890-895
language	English Chinese
source	In Chinese Journal of Contemporary Neurology and Neurosurgery 15(2015), 11, Seite 890-895 volume:15 year:2015 number:11 pages:890-895
sourceStr	In Chinese Journal of Contemporary Neurology and Neurosurgery 15(2015), 11, Seite 890-895 volume:15 year:2015 number:11 pages:890-895
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal Neurology. Diseases of the nervous system
isfreeaccess_bool	true
container_title	Chinese Journal of Contemporary Neurology and Neurosurgery
authorswithroles_txt_mv	Nan-nan ZHANG @@aut@@ Lin CONG @@aut@@ Xiu-ying FANG @@aut@@ Xue-mei FENG @@aut@@ Xi-ke ZHU @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	741171848
id	DOAJ040270637
language_de	englisch chinesisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ040270637</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230308034745.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2015 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ040270637</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJbca6bc4634774be28fb4d9cc7e5aa0d6</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield><subfield code="a">chi</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC346-429</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Nan-nan ZHANG</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Epilepsy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Kainic acid</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Diet therapy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ketone bodies</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hippocampus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Neurons</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Disease models, animal</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Neurology. 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callnumber-first	R - Medicine
author	Nan-nan ZHANG
spellingShingle	Nan-nan ZHANG misc RC346-429 misc Epilepsy misc Kainic acid misc Diet therapy misc Ketone bodies misc Hippocampus misc Neurons misc Disease models, animal misc Neurology. Diseases of the nervous system Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
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topic_title	RC346-429 Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats Epilepsy Kainic acid Diet therapy Ketone bodies Hippocampus Neurons Disease models, animal
topic	misc RC346-429 misc Epilepsy misc Kainic acid misc Diet therapy misc Ketone bodies misc Hippocampus misc Neurons misc Disease models, animal misc Neurology. Diseases of the nervous system
topic_unstemmed	misc RC346-429 misc Epilepsy misc Kainic acid misc Diet therapy misc Ketone bodies misc Hippocampus misc Neurons misc Disease models, animal misc Neurology. Diseases of the nervous system
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title	Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
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title_full	Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
author_sort	Nan-nan ZHANG
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author_browse	Nan-nan ZHANG Lin CONG Xiu-ying FANG Xue-mei FENG Xi-ke ZHU
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author-letter	Nan-nan ZHANG
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title_sort	study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
callnumber	RC346-429
title_auth	Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
abstract	<p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p<
abstractGer	<p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p<
abstract_unstemmed	<p<<strong<Objective</strong< To investigate the protective effects of ketogenic diet on hippocampal neurons of kainic acid (KA)-induced epileptic rats. <strong<Methods </strong< A total of 40 healthy male specific pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, with each group containing 10 rats. Epileptic rat models were formed by injection of KA through lateral ventricle with brain stereotactic instrument. According to Racine classification, epileptic seizures in rats above grade Ⅳ were defined successful. Then the rats were given different dietary treatment: Group C with normal saline and normal diet, Group K with normal saline and ketogenic diet, Group E with KA and normal diet, Group EK with KA and ketogenic diet. All rats were observed for 21 d, and were recorded each body weight. The epileptic seizure frequency and duration were observed at 12:00-15:00 daily. At the 21st day, all rats were put to death, and the brain hippocampus tissues were separated. Neuron injury of rat hippocampal CA3 region in Group E and EK was observed by HE staining. Normal neuron number of rat hippocampal CA3 region in Group E and EK was counted by Nissl staining. <strong< Results</strong< Group C and K had no epileptic seizures, and the neuron number in hippocampal CA3 region was normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number of seizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet was decreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-day normal diet (<em<t</em< = 6.606, <em<P </em<= 0.000). The seizure duration in Group EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened compared to Group E [(335.00 ± 14.21) s] after 21-day normal diet (<em<t</em< = 17.011, <em<P</em< = 0.000). The normal neuron number in hippocampal CA3 region in Group EK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), with statistically significant difference (<em<t</em< = 9.614, <em<P</em< = 0.000). <strong<Conclusions </strong< Ketogenic diet has protective effect on hippocampal neurons of kainic acid-induced epileptic rats.</p<<p< </p<<p<<strong<DOI: </strong<10.3969/j.issn.1672-6731.2015.11.011</p<
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title_short	Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats
url	https://doaj.org/article/bca6bc4634774be28fb4d9cc7e5aa0d6 http://www.cjcnn.org/index.php/cjcnn/article/view/1307 https://doaj.org/toc/1672-6731
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Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats

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