Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer

Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EP...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zhongbao Ma [verfasserIn] Mengmeng Xu [verfasserIn] Qiong Wang [verfasserIn] Feng Wang [verfasserIn] Huihua Zheng [verfasserIn] Zhenghua Gu [verfasserIn] Youran Li [verfasserIn] Guiyang Shi [verfasserIn] Zhongyang Ding [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 10(2019)
Übergeordnetes Werk:	volume:10 ; year:2019

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2019.02306

Katalog-ID:	DOAJ040305988

Internformat


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allfields	10.3389/fmicb.2019.02306 doi (DE-627)DOAJ040305988 (DE-599)DOAJc936dc0dc46d4cc9847c520609471173 DE-627 ger DE-627 rakwb eng QR1-502 Zhongbao Ma verfasserin aut Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost. Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy Microbiology Zhongbao Ma verfasserin aut Mengmeng Xu verfasserin aut Mengmeng Xu verfasserin aut Qiong Wang verfasserin aut Qiong Wang verfasserin aut Feng Wang verfasserin aut Huihua Zheng verfasserin aut Zhenghua Gu verfasserin aut Zhenghua Gu verfasserin aut Youran Li verfasserin aut Youran Li verfasserin aut Guiyang Shi verfasserin aut Guiyang Shi verfasserin aut Zhongyang Ding verfasserin aut Zhongyang Ding verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 10(2019) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:10 year:2019 https://doi.org/10.3389/fmicb.2019.02306 kostenfrei https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 kostenfrei https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
spelling	10.3389/fmicb.2019.02306 doi (DE-627)DOAJ040305988 (DE-599)DOAJc936dc0dc46d4cc9847c520609471173 DE-627 ger DE-627 rakwb eng QR1-502 Zhongbao Ma verfasserin aut Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost. Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy Microbiology Zhongbao Ma verfasserin aut Mengmeng Xu verfasserin aut Mengmeng Xu verfasserin aut Qiong Wang verfasserin aut Qiong Wang verfasserin aut Feng Wang verfasserin aut Huihua Zheng verfasserin aut Zhenghua Gu verfasserin aut Zhenghua Gu verfasserin aut Youran Li verfasserin aut Youran Li verfasserin aut Guiyang Shi verfasserin aut Guiyang Shi verfasserin aut Zhongyang Ding verfasserin aut Zhongyang Ding verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 10(2019) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:10 year:2019 https://doi.org/10.3389/fmicb.2019.02306 kostenfrei https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 kostenfrei https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
allfields_unstemmed	10.3389/fmicb.2019.02306 doi (DE-627)DOAJ040305988 (DE-599)DOAJc936dc0dc46d4cc9847c520609471173 DE-627 ger DE-627 rakwb eng QR1-502 Zhongbao Ma verfasserin aut Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost. Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy Microbiology Zhongbao Ma verfasserin aut Mengmeng Xu verfasserin aut Mengmeng Xu verfasserin aut Qiong Wang verfasserin aut Qiong Wang verfasserin aut Feng Wang verfasserin aut Huihua Zheng verfasserin aut Zhenghua Gu verfasserin aut Zhenghua Gu verfasserin aut Youran Li verfasserin aut Youran Li verfasserin aut Guiyang Shi verfasserin aut Guiyang Shi verfasserin aut Zhongyang Ding verfasserin aut Zhongyang Ding verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 10(2019) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:10 year:2019 https://doi.org/10.3389/fmicb.2019.02306 kostenfrei https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 kostenfrei https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
allfieldsGer	10.3389/fmicb.2019.02306 doi (DE-627)DOAJ040305988 (DE-599)DOAJc936dc0dc46d4cc9847c520609471173 DE-627 ger DE-627 rakwb eng QR1-502 Zhongbao Ma verfasserin aut Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost. Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy Microbiology Zhongbao Ma verfasserin aut Mengmeng Xu verfasserin aut Mengmeng Xu verfasserin aut Qiong Wang verfasserin aut Qiong Wang verfasserin aut Feng Wang verfasserin aut Huihua Zheng verfasserin aut Zhenghua Gu verfasserin aut Zhenghua Gu verfasserin aut Youran Li verfasserin aut Youran Li verfasserin aut Guiyang Shi verfasserin aut Guiyang Shi verfasserin aut Zhongyang Ding verfasserin aut Zhongyang Ding verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 10(2019) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:10 year:2019 https://doi.org/10.3389/fmicb.2019.02306 kostenfrei https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 kostenfrei https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
allfieldsSound	10.3389/fmicb.2019.02306 doi (DE-627)DOAJ040305988 (DE-599)DOAJc936dc0dc46d4cc9847c520609471173 DE-627 ger DE-627 rakwb eng QR1-502 Zhongbao Ma verfasserin aut Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost. Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy Microbiology Zhongbao Ma verfasserin aut Mengmeng Xu verfasserin aut Mengmeng Xu verfasserin aut Qiong Wang verfasserin aut Qiong Wang verfasserin aut Feng Wang verfasserin aut Huihua Zheng verfasserin aut Zhenghua Gu verfasserin aut Zhenghua Gu verfasserin aut Youran Li verfasserin aut Youran Li verfasserin aut Guiyang Shi verfasserin aut Guiyang Shi verfasserin aut Zhongyang Ding verfasserin aut Zhongyang Ding verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 10(2019) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:10 year:2019 https://doi.org/10.3389/fmicb.2019.02306 kostenfrei https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 kostenfrei https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019
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authorswithroles_txt_mv	Zhongbao Ma @@aut@@ Mengmeng Xu @@aut@@ Qiong Wang @@aut@@ Feng Wang @@aut@@ Huihua Zheng @@aut@@ Zhenghua Gu @@aut@@ Youran Li @@aut@@ Guiyang Shi @@aut@@ Zhongyang Ding @@aut@@
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callnumber-first	Q - Science
author	Zhongbao Ma
spellingShingle	Zhongbao Ma misc QR1-502 misc Ganoderma lucidum misc extracellular polysaccharide misc L-phenylalanine misc cell wall misc efficient strategy misc Microbiology Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer
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topic_title	QR1-502 Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer Ganoderma lucidum extracellular polysaccharide L-phenylalanine cell wall efficient strategy
topic	misc QR1-502 misc Ganoderma lucidum misc extracellular polysaccharide misc L-phenylalanine misc cell wall misc efficient strategy misc Microbiology
topic_unstemmed	misc QR1-502 misc Ganoderma lucidum misc extracellular polysaccharide misc L-phenylalanine misc cell wall misc efficient strategy misc Microbiology
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title	Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer
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title_full	Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer
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title_sort	development of an efficient strategy to improve extracellular polysaccharide production of ganoderma lucidum using l-phenylalanine as an enhancer
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title_auth	Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer
abstract	Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost.
abstractGer	Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost.
abstract_unstemmed	Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost.
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title_short	Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer
url	https://doi.org/10.3389/fmicb.2019.02306 https://doaj.org/article/c936dc0dc46d4cc9847c520609471173 https://www.frontiersin.org/article/10.3389/fmicb.2019.02306/full https://doaj.org/toc/1664-302X
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up_date	2024-07-03T14:06:40.778Z
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Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. 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Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer

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