Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method
Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Ur...
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| Autor*in: |
Javad Mohammadi [verfasserIn] Kumarss Amini [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Persisch |
| Erschienen: |
2017 |
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| Übergeordnetes Werk: |
In: Journal of Fasa University of Medical Sciences - Fasa University of Medical Sciences, 2013, 7(2017), 1, Seite 128-133 |
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volume:7 ; year:2017 ; number:1 ; pages:128-133 |
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| 520 | |a Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary | ||
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(DE-627)DOAJ040345351 (DE-599)DOAJ18e28cac465d4720891a9ae3c1952235 DE-627 ger DE-627 rakwb per R5-920 Javad Mohammadi verfasserin aut Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary UPEC iha iroN ompT multiplex-PCR Medicine (General) Kumarss Amini verfasserin aut In Journal of Fasa University of Medical Sciences Fasa University of Medical Sciences, 2013 7(2017), 1, Seite 128-133 (DE-627)1760645834 22287329 nnns volume:7 year:2017 number:1 pages:128-133 https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 kostenfrei http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/2228-5105 Journal toc kostenfrei https://doaj.org/toc/2228-7329 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 7 2017 1 128-133 |
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(DE-627)DOAJ040345351 (DE-599)DOAJ18e28cac465d4720891a9ae3c1952235 DE-627 ger DE-627 rakwb per R5-920 Javad Mohammadi verfasserin aut Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary UPEC iha iroN ompT multiplex-PCR Medicine (General) Kumarss Amini verfasserin aut In Journal of Fasa University of Medical Sciences Fasa University of Medical Sciences, 2013 7(2017), 1, Seite 128-133 (DE-627)1760645834 22287329 nnns volume:7 year:2017 number:1 pages:128-133 https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 kostenfrei http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/2228-5105 Journal toc kostenfrei https://doaj.org/toc/2228-7329 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 7 2017 1 128-133 |
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(DE-627)DOAJ040345351 (DE-599)DOAJ18e28cac465d4720891a9ae3c1952235 DE-627 ger DE-627 rakwb per R5-920 Javad Mohammadi verfasserin aut Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary UPEC iha iroN ompT multiplex-PCR Medicine (General) Kumarss Amini verfasserin aut In Journal of Fasa University of Medical Sciences Fasa University of Medical Sciences, 2013 7(2017), 1, Seite 128-133 (DE-627)1760645834 22287329 nnns volume:7 year:2017 number:1 pages:128-133 https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 kostenfrei http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/2228-5105 Journal toc kostenfrei https://doaj.org/toc/2228-7329 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 7 2017 1 128-133 |
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(DE-627)DOAJ040345351 (DE-599)DOAJ18e28cac465d4720891a9ae3c1952235 DE-627 ger DE-627 rakwb per R5-920 Javad Mohammadi verfasserin aut Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary UPEC iha iroN ompT multiplex-PCR Medicine (General) Kumarss Amini verfasserin aut In Journal of Fasa University of Medical Sciences Fasa University of Medical Sciences, 2013 7(2017), 1, Seite 128-133 (DE-627)1760645834 22287329 nnns volume:7 year:2017 number:1 pages:128-133 https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 kostenfrei http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/2228-5105 Journal toc kostenfrei https://doaj.org/toc/2228-7329 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 7 2017 1 128-133 |
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(DE-627)DOAJ040345351 (DE-599)DOAJ18e28cac465d4720891a9ae3c1952235 DE-627 ger DE-627 rakwb per R5-920 Javad Mohammadi verfasserin aut Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary UPEC iha iroN ompT multiplex-PCR Medicine (General) Kumarss Amini verfasserin aut In Journal of Fasa University of Medical Sciences Fasa University of Medical Sciences, 2013 7(2017), 1, Seite 128-133 (DE-627)1760645834 22287329 nnns volume:7 year:2017 number:1 pages:128-133 https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 kostenfrei http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/2228-5105 Journal toc kostenfrei https://doaj.org/toc/2228-7329 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 7 2017 1 128-133 |
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Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary |
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Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary |
| abstract_unstemmed |
Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary |
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Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method |
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https://doaj.org/article/18e28cac465d4720891a9ae3c1952235 http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1 https://doaj.org/toc/2228-5105 https://doaj.org/toc/2228-7329 |
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2024-07-03T14:19:38.955Z |
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