Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum

The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T...
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Gespeichert in:

Autor*in:	Mihail Kantor [verfasserIn] Radu Sestras [verfasserIn] Kamal Chowdhury [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Spanisch ; Portugiesisch

Erschienen:	2013

Schlagwörter:	expressão gênica transformação genética malária vacina a partir de plantas

Übergeordnetes Werk:	In: Pesquisa Agropecuária Brasileira - Embrapa Informação Tecnológica, 2004, 48(2013), 1, Seite 73-79
Übergeordnetes Werk:	volume:48 ; year:2013 ; number:1 ; pages:73-79

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/S0100-204X2013000100010

Katalog-ID:	DOAJ04036996X

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520			\|a The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
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allfields	10.1590/S0100-204X2013000100010 doi (DE-627)DOAJ04036996X (DE-599)DOAJef3ba1f0df8f4b219def00256ec16742 DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations. expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doi.org/10.1590/S0100-204X2013000100010 kostenfrei https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
spelling	10.1590/S0100-204X2013000100010 doi (DE-627)DOAJ04036996X (DE-599)DOAJef3ba1f0df8f4b219def00256ec16742 DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations. expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doi.org/10.1590/S0100-204X2013000100010 kostenfrei https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfields_unstemmed	10.1590/S0100-204X2013000100010 doi (DE-627)DOAJ04036996X (DE-599)DOAJef3ba1f0df8f4b219def00256ec16742 DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations. expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doi.org/10.1590/S0100-204X2013000100010 kostenfrei https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfieldsGer	10.1590/S0100-204X2013000100010 doi (DE-627)DOAJ04036996X (DE-599)DOAJef3ba1f0df8f4b219def00256ec16742 DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations. expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doi.org/10.1590/S0100-204X2013000100010 kostenfrei https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfieldsSound	10.1590/S0100-204X2013000100010 doi (DE-627)DOAJ04036996X (DE-599)DOAJef3ba1f0df8f4b219def00256ec16742 DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations. expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doi.org/10.1590/S0100-204X2013000100010 kostenfrei https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
language	English Spanish Portuguese
source	In Pesquisa Agropecuária Brasileira 48(2013), 1, Seite 73-79 volume:48 year:2013 number:1 pages:73-79
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topic_facet	expressão gênica transformação genética malária vacina a partir de plantas Agriculture (General)
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container_title	Pesquisa Agropecuária Brasileira
authorswithroles_txt_mv	Mihail Kantor @@aut@@ Radu Sestras @@aut@@ Kamal Chowdhury @@aut@@
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callnumber-first	S - Agriculture
author	Mihail Kantor
spellingShingle	Mihail Kantor misc S1-972 misc expressão gênica misc transformação genética misc malária misc vacina a partir de plantas misc Agriculture (General) Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum
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topic_title	S1-972 Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum expressão gênica transformação genética malária vacina a partir de plantas
topic	misc S1-972 misc expressão gênica misc transformação genética misc malária misc vacina a partir de plantas misc Agriculture (General)
topic_unstemmed	misc S1-972 misc expressão gênica misc transformação genética misc malária misc vacina a partir de plantas misc Agriculture (General)
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title	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum
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title_full	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum
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title_sort	transgenic tomato plants expressing the antigen gene pfcp-2.9 of plasmodium falciparum
callnumber	S1-972
title_auth	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum
abstract	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
abstractGer	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
abstract_unstemmed	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
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title_short	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum
url	https://doi.org/10.1590/S0100-204X2013000100010 https://doaj.org/article/ef3ba1f0df8f4b219def00256ec16742 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010&lng=en&tlng=en https://doaj.org/toc/1678-3921
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