Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs

<p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Davis Peter G [verfasserIn] Cole Timothy J [verfasserIn] Crossley Kelly [verfasserIn] Zahra Valerie A [verfasserIn] Probyn Megan E [verfasserIn] Wallace Megan J [verfasserIn] Morley Colin J [verfasserIn] Hooper Stuart B [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: Respiratory Research - BMC, 2003, 10(2009), 1, p 19
Übergeordnetes Werk:	volume:10 ; year:2009 ; number:1, p 19

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1465-9921-10-19

Katalog-ID:	DOAJ040515389

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245	1	0	\|a Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
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520			\|a <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<
653		0	\|a Diseases of the respiratory system
700	0		\|a Cole Timothy J \|e verfasserin \|4 aut
700	0		\|a Crossley Kelly \|e verfasserin \|4 aut
700	0		\|a Zahra Valerie A \|e verfasserin \|4 aut
700	0		\|a Probyn Megan E \|e verfasserin \|4 aut
700	0		\|a Wallace Megan J \|e verfasserin \|4 aut
700	0		\|a Morley Colin J \|e verfasserin \|4 aut
700	0		\|a Hooper Stuart B \|e verfasserin \|4 aut
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author_variant	d p g dpg c t j ctj c k ck z v a zva p m e pme w m j wmj m c j mcj h s b hsb
matchkey_str	article:1465993X:2009----::alboakradoetamdaosfetltoidcdugn
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publishDate	2009
allfields	10.1186/1465-9921-10-19 doi (DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe DE-627 ger DE-627 rakwb eng RC705-779 Davis Peter G verfasserin aut Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Diseases of the respiratory system Cole Timothy J verfasserin aut Crossley Kelly verfasserin aut Zahra Valerie A verfasserin aut Probyn Megan E verfasserin aut Wallace Megan J verfasserin aut Morley Colin J verfasserin aut Hooper Stuart B verfasserin aut In Respiratory Research BMC, 2003 10(2009), 1, p 19 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:10 year:2009 number:1, p 19 https://doi.org/10.1186/1465-9921-10-19 kostenfrei https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe kostenfrei http://respiratory-research.com/content/10/1/19 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 19
spelling	10.1186/1465-9921-10-19 doi (DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe DE-627 ger DE-627 rakwb eng RC705-779 Davis Peter G verfasserin aut Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Diseases of the respiratory system Cole Timothy J verfasserin aut Crossley Kelly verfasserin aut Zahra Valerie A verfasserin aut Probyn Megan E verfasserin aut Wallace Megan J verfasserin aut Morley Colin J verfasserin aut Hooper Stuart B verfasserin aut In Respiratory Research BMC, 2003 10(2009), 1, p 19 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:10 year:2009 number:1, p 19 https://doi.org/10.1186/1465-9921-10-19 kostenfrei https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe kostenfrei http://respiratory-research.com/content/10/1/19 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 19
allfields_unstemmed	10.1186/1465-9921-10-19 doi (DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe DE-627 ger DE-627 rakwb eng RC705-779 Davis Peter G verfasserin aut Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Diseases of the respiratory system Cole Timothy J verfasserin aut Crossley Kelly verfasserin aut Zahra Valerie A verfasserin aut Probyn Megan E verfasserin aut Wallace Megan J verfasserin aut Morley Colin J verfasserin aut Hooper Stuart B verfasserin aut In Respiratory Research BMC, 2003 10(2009), 1, p 19 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:10 year:2009 number:1, p 19 https://doi.org/10.1186/1465-9921-10-19 kostenfrei https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe kostenfrei http://respiratory-research.com/content/10/1/19 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 19
allfieldsGer	10.1186/1465-9921-10-19 doi (DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe DE-627 ger DE-627 rakwb eng RC705-779 Davis Peter G verfasserin aut Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Diseases of the respiratory system Cole Timothy J verfasserin aut Crossley Kelly verfasserin aut Zahra Valerie A verfasserin aut Probyn Megan E verfasserin aut Wallace Megan J verfasserin aut Morley Colin J verfasserin aut Hooper Stuart B verfasserin aut In Respiratory Research BMC, 2003 10(2009), 1, p 19 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:10 year:2009 number:1, p 19 https://doi.org/10.1186/1465-9921-10-19 kostenfrei https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe kostenfrei http://respiratory-research.com/content/10/1/19 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 19
allfieldsSound	10.1186/1465-9921-10-19 doi (DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe DE-627 ger DE-627 rakwb eng RC705-779 Davis Peter G verfasserin aut Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p< Diseases of the respiratory system Cole Timothy J verfasserin aut Crossley Kelly verfasserin aut Zahra Valerie A verfasserin aut Probyn Megan E verfasserin aut Wallace Megan J verfasserin aut Morley Colin J verfasserin aut Hooper Stuart B verfasserin aut In Respiratory Research BMC, 2003 10(2009), 1, p 19 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:10 year:2009 number:1, p 19 https://doi.org/10.1186/1465-9921-10-19 kostenfrei https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe kostenfrei http://respiratory-research.com/content/10/1/19 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 19
language	English
source	In Respiratory Research 10(2009), 1, p 19 volume:10 year:2009 number:1, p 19
sourceStr	In Respiratory Research 10(2009), 1, p 19 volume:10 year:2009 number:1, p 19
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Diseases of the respiratory system
isfreeaccess_bool	true
container_title	Respiratory Research
authorswithroles_txt_mv	Davis Peter G @@aut@@ Cole Timothy J @@aut@@ Crossley Kelly @@aut@@ Zahra Valerie A @@aut@@ Probyn Megan E @@aut@@ Wallace Megan J @@aut@@ Morley Colin J @@aut@@ Hooper Stuart B @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	326646485
id	DOAJ040515389
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ040515389</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230503061426.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2009 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1465-9921-10-19</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ040515389</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC705-779</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Davis Peter G</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Diseases of the respiratory system</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cole Timothy J</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Crossley Kelly</subfield><subfield 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callnumber-first	R - Medicine
author	Davis Peter G
spellingShingle	Davis Peter G misc RC705-779 misc Diseases of the respiratory system Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
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topic_title	RC705-779 Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
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title	Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
ctrlnum	(DE-627)DOAJ040515389 (DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe
title_full	Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
author_sort	Davis Peter G
journal	Respiratory Research
journalStr	Respiratory Research
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lang_code	eng
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author_browse	Davis Peter G Cole Timothy J Crossley Kelly Zahra Valerie A Probyn Megan E Wallace Megan J Morley Colin J Hooper Stuart B
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doi_str_mv	10.1186/1465-9921-10-19
author2-role	verfasserin
title_sort	early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
callnumber	RC705-779
title_auth	Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
abstract	<p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<
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container_issue	1, p 19
title_short	Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
url	https://doi.org/10.1186/1465-9921-10-19 https://doaj.org/article/79cf88396f2c4f5595914502b809bdfe http://respiratory-research.com/content/10/1/19 https://doaj.org/toc/1465-9921
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up_date	2024-07-03T15:15:48.995Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ040515389</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230503061426.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2009 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1465-9921-10-19</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ040515389</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ79cf88396f2c4f5595914502b809bdfe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC705-779</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Davis Peter G</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<Bronchopulmonary dysplasia (BPD) is closely associated with ventilator-induced lung injury (VILI) in very preterm infants. The greatest risk of VILI may be in the immediate period after birth, when the lungs are surfactant deficient, still partially filled with liquid and not uniformly aerated. However, there have been very few studies that have examined this immediate post-birth period and identified the initial injury-related pathways that are activated. We aimed to determine if the early response genes; connective tissue growth factor (<it<CTGF</it<), cysteine rich-61 (<it<CYR61</it<) and early growth response 1 (<it<EGR1</it<), were rapidly induced by VILI in preterm lambs and whether ventilation with different tidal volumes caused different inflammatory cytokine and early response gene expression.</p< <p<Methods</p< <p<To identify early markers of VILI, preterm lambs (132 d gestational age; GA, term ~147 d) were resuscitated with an injurious ventilation strategy (V<sub<T </sub<20 mL/kg for 15 min) then gently ventilated (5 mL/kg) for 15, 30, 60 or 120 min (n = 4 in each). To determine if early response genes and inflammatory cytokines were differentially regulated by different ventilation strategies, separate groups of preterm lambs (125 d GA; n = 5 in each) were ventilated from birth with a V<sub<T </sub<of 5 (VG5) or 10 mL/kg (VG10) for 135 minutes. Lung gene expression levels were compared to levels prior to ventilation in age-matched control fetuses.</p< <p<Results</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<lung mRNA levels were increased ~25, 50 and 120-fold respectively (p < 0.05), within 30 minutes of injurious ventilation. VG5 and VG10 caused significant increases in <it<CTGF</it<, <it<CYR61</it<, <it<EGR1</it<, <it<IL1-β</it<, <it<IL-6 </it<and <it<IL-8 </it<mRNA levels compared to control levels. <it<CTGF, CYR61, IL-6 </it<and <it<IL-8 </it<expression levels were higher in VG10 than VG5 lambs; although only the <it<IL-6 </it<and <it<CYR61 </it<mRNA levels reached significance.</p< <p<Conclusion</p< <p<<it<CTGF, CYR61 </it<and <it<EGR1 </it<may be novel early markers of lung injury and mechanical ventilation from birth using relatively low tidal volumes may be less injurious than using higher tidal volumes.</p<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Diseases of the respiratory system</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cole Timothy J</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Crossley Kelly</subfield><subfield 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Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs

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