Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR

Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiologica...
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Autor*in:	José Luis Hernández Flores [verfasserIn] Diana Salinas Landaverde [verfasserIn] Yonuen Pacheco Huerta [verfasserIn] Vania Lizeth Guerra Castillo [verfasserIn] María de los Ángeles Barrios Sánchez [verfasserIn] Iván Arvizu Hernández [verfasserIn] Miguel Ángel Ramos López [verfasserIn] Erika Álvarez Hidalgo [verfasserIn] George H. Jones [verfasserIn] Juan Campos Guillén [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	tRNA MALDI-TOF MS bee pollen

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 8(2020), 4, p 524
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:4, p 524

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms8040524

Katalog-ID:	DOAJ040614611

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author_variant	j l h f jlhf d s l dsl y p h yph v l g c vlgc m d l á b s mdlábs i a h iah m á r l márl e á h eáh g h j ghj j c g jcg
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allfields	10.3390/microorganisms8040524 doi (DE-627)DOAJ040614611 (DE-599)DOAJ437b1df7b63d47639b4d0c0ae523a8f6 DE-627 ger DE-627 rakwb eng QH301-705.5 José Luis Hernández Flores verfasserin aut Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA<sup<Cys</sup< gene and specific genes associated with tRNA<sup<Cys</sup< to detect <i<Bacillus cereus sensu lato</i< (<i<B. cereus s.l.</i<). This tRNA<sup<Cys</sup<-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to <i<B. cereus s.l.,</i< and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to <i<B. cereus, B. thuringiensis</i<, and <i<B. bombysepticus</i<. Our results indicate that the tRNA<sup<Cys</sup<-PCR, combined with other molecular tools, will be a useful approach for identifying <i<B. cereus s.l.</i< and will assist in controlling the spread of potential pathogens. <i<Bacillus cereus</i< <i<Bacillus thuringiensis</i< <i<Bacillus bombysepticus</i< tRNA<sup<Cys</sup<-PCR MALDI-TOF MS bee pollen Biology (General) Diana Salinas Landaverde verfasserin aut Yonuen Pacheco Huerta verfasserin aut Vania Lizeth Guerra Castillo verfasserin aut María de los Ángeles Barrios Sánchez verfasserin aut Iván Arvizu Hernández verfasserin aut Miguel Ángel Ramos López verfasserin aut Erika Álvarez Hidalgo verfasserin aut George H. Jones verfasserin aut Juan Campos Guillén verfasserin aut In Microorganisms MDPI AG, 2013 8(2020), 4, p 524 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:8 year:2020 number:4, p 524 https://doi.org/10.3390/microorganisms8040524 kostenfrei https://doaj.org/article/437b1df7b63d47639b4d0c0ae523a8f6 kostenfrei https://www.mdpi.com/2076-2607/8/4/524 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 524
spelling	10.3390/microorganisms8040524 doi (DE-627)DOAJ040614611 (DE-599)DOAJ437b1df7b63d47639b4d0c0ae523a8f6 DE-627 ger DE-627 rakwb eng QH301-705.5 José Luis Hernández Flores verfasserin aut Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA<sup<Cys</sup< gene and specific genes associated with tRNA<sup<Cys</sup< to detect <i<Bacillus cereus sensu lato</i< (<i<B. cereus s.l.</i<). This tRNA<sup<Cys</sup<-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to <i<B. cereus s.l.,</i< and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to <i<B. cereus, B. thuringiensis</i<, and <i<B. bombysepticus</i<. Our results indicate that the tRNA<sup<Cys</sup<-PCR, combined with other molecular tools, will be a useful approach for identifying <i<B. cereus s.l.</i< and will assist in controlling the spread of potential pathogens. <i<Bacillus cereus</i< <i<Bacillus thuringiensis</i< <i<Bacillus bombysepticus</i< tRNA<sup<Cys</sup<-PCR MALDI-TOF MS bee pollen Biology (General) Diana Salinas Landaverde verfasserin aut Yonuen Pacheco Huerta verfasserin aut Vania Lizeth Guerra Castillo verfasserin aut María de los Ángeles Barrios Sánchez verfasserin aut Iván Arvizu Hernández verfasserin aut Miguel Ángel Ramos López verfasserin aut Erika Álvarez Hidalgo verfasserin aut George H. Jones verfasserin aut Juan Campos Guillén verfasserin aut In Microorganisms MDPI AG, 2013 8(2020), 4, p 524 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:8 year:2020 number:4, p 524 https://doi.org/10.3390/microorganisms8040524 kostenfrei https://doaj.org/article/437b1df7b63d47639b4d0c0ae523a8f6 kostenfrei https://www.mdpi.com/2076-2607/8/4/524 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 524
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topic_facet	<i<Bacillus cereus</i< <i<Bacillus thuringiensis</i< <i<Bacillus bombysepticus</i< tRNA<sup<Cys</sup<-PCR MALDI-TOF MS bee pollen Biology (General)
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issn	20762607
topic_title	QH301-705.5 Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR <i<Bacillus cereus</i< <i<Bacillus thuringiensis</i< <i<Bacillus bombysepticus</i< tRNA<sup<Cys</sup<-PCR MALDI-TOF MS bee pollen
topic	misc QH301-705.5 misc <i<Bacillus cereus</i< misc <i<Bacillus thuringiensis</i< misc <i<Bacillus bombysepticus</i< misc tRNA<sup<Cys</sup<-PCR misc MALDI-TOF MS misc bee pollen misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Bacillus cereus</i< misc <i<Bacillus thuringiensis</i< misc <i<Bacillus bombysepticus</i< misc tRNA<sup<Cys</sup<-PCR misc MALDI-TOF MS misc bee pollen misc Biology (General)
topic_browse	misc QH301-705.5 misc <i<Bacillus cereus</i< misc <i<Bacillus thuringiensis</i< misc <i<Bacillus bombysepticus</i< misc tRNA<sup<Cys</sup<-PCR misc MALDI-TOF MS misc bee pollen misc Biology (General)
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hierarchy_parent_title	Microorganisms
hierarchy_parent_id	750370696
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title	Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR
ctrlnum	(DE-627)DOAJ040614611 (DE-599)DOAJ437b1df7b63d47639b4d0c0ae523a8f6
title_full	Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR
author_sort	José Luis Hernández Flores
journal	Microorganisms
journalStr	Microorganisms
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lang_code	eng
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author_browse	José Luis Hernández Flores Diana Salinas Landaverde Yonuen Pacheco Huerta Vania Lizeth Guerra Castillo María de los Ángeles Barrios Sánchez Iván Arvizu Hernández Miguel Ángel Ramos López Erika Álvarez Hidalgo George H. Jones Juan Campos Guillén
container_volume	8
class	QH301-705.5
format_se	Elektronische Aufsätze
author-letter	José Luis Hernández Flores
doi_str_mv	10.3390/microorganisms8040524
author2-role	verfasserin
title_sort	phylogenetic analysis of <i<bacillus cereus sensu lato</i< isolates from commercial bee pollen using trna<sup<cys</sup<-pcr
callnumber	QH301-705.5
title_auth	Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR
abstract	Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA<sup<Cys</sup< gene and specific genes associated with tRNA<sup<Cys</sup< to detect <i<Bacillus cereus sensu lato</i< (<i<B. cereus s.l.</i<). This tRNA<sup<Cys</sup<-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to <i<B. cereus s.l.,</i< and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to <i<B. cereus, B. thuringiensis</i<, and <i<B. bombysepticus</i<. Our results indicate that the tRNA<sup<Cys</sup<-PCR, combined with other molecular tools, will be a useful approach for identifying <i<B. cereus s.l.</i< and will assist in controlling the spread of potential pathogens.
abstractGer	Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA<sup<Cys</sup< gene and specific genes associated with tRNA<sup<Cys</sup< to detect <i<Bacillus cereus sensu lato</i< (<i<B. cereus s.l.</i<). This tRNA<sup<Cys</sup<-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to <i<B. cereus s.l.,</i< and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to <i<B. cereus, B. thuringiensis</i<, and <i<B. bombysepticus</i<. Our results indicate that the tRNA<sup<Cys</sup<-PCR, combined with other molecular tools, will be a useful approach for identifying <i<B. cereus s.l.</i< and will assist in controlling the spread of potential pathogens.
abstract_unstemmed	Endospore-forming bacteria related to the <i<Bacillus cereus</i< group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA<sup<Cys</sup< gene and specific genes associated with tRNA<sup<Cys</sup< to detect <i<Bacillus cereus sensu lato</i< (<i<B. cereus s.l.</i<). This tRNA<sup<Cys</sup<-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to <i<B. cereus s.l.,</i< and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to <i<B. cereus, B. thuringiensis</i<, and <i<B. bombysepticus</i<. Our results indicate that the tRNA<sup<Cys</sup<-PCR, combined with other molecular tools, will be a useful approach for identifying <i<B. cereus s.l.</i< and will assist in controlling the spread of potential pathogens.
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container_issue	4, p 524
title_short	Phylogenetic Analysis of <i<Bacillus cereus sensu lato</i< Isolates from Commercial Bee Pollen Using tRNA<sup<Cys</sup<-PCR
url	https://doi.org/10.3390/microorganisms8040524 https://doaj.org/article/437b1df7b63d47639b4d0c0ae523a8f6 https://www.mdpi.com/2076-2607/8/4/524 https://doaj.org/toc/2076-2607
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author2	Diana Salinas Landaverde Yonuen Pacheco Huerta Vania Lizeth Guerra Castillo María de los Ángeles Barrios Sánchez Iván Arvizu Hernández Miguel Ángel Ramos López Erika Álvarez Hidalgo George H. Jones Juan Campos Guillén
author2Str	Diana Salinas Landaverde Yonuen Pacheco Huerta Vania Lizeth Guerra Castillo María de los Ángeles Barrios Sánchez Iván Arvizu Hernández Miguel Ángel Ramos López Erika Álvarez Hidalgo George H. Jones Juan Campos Guillén
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up_date	2024-07-03T15:48:54.366Z
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