Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy

Assembly of eukaryotic ribosomal subunits is a very complex and sequential process that starts in the nucleolus and finishes in the cytoplasm with the formation of functional ribosomes. Over the past few years, characterization of the many molecular events underlying eukaryotic ribosome biogenesis h...
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Gespeichert in:

Autor*in:	Ramtin Shayan [verfasserIn] Dana Rinaldi [verfasserIn] Natacha Larburu [verfasserIn] Laura Plassart [verfasserIn] Stéphanie Balor [verfasserIn] David Bouyssié [verfasserIn] Simon Lebaron [verfasserIn] Julien Marcoux [verfasserIn] Pierre-Emmanuel Gleizes [verfasserIn] Célia Plisson-Chastang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	ribosome assembly small ribosomal subunit bottom-up proteomics cryo-em single particle analysis

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 25(2020), 5, p 1125
Übergeordnetes Werk:	volume:25 ; year:2020 ; number:5, p 1125

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules25051125

Katalog-ID:	DOAJ040843858

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language	English
source	In Molecules 25(2020), 5, p 1125 volume:25 year:2020 number:5, p 1125
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topic_facet	ribosome assembly small ribosomal subunit bottom-up proteomics cryo-em single particle analysis Organic chemistry
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authorswithroles_txt_mv	Ramtin Shayan @@aut@@ Dana Rinaldi @@aut@@ Natacha Larburu @@aut@@ Laura Plassart @@aut@@ Stéphanie Balor @@aut@@ David Bouyssié @@aut@@ Simon Lebaron @@aut@@ Julien Marcoux @@aut@@ Pierre-Emmanuel Gleizes @@aut@@ Célia Plisson-Chastang @@aut@@
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callnumber-first	Q - Science
author	Ramtin Shayan
spellingShingle	Ramtin Shayan misc QD241-441 misc ribosome assembly misc small ribosomal subunit misc bottom-up proteomics misc cryo-em misc single particle analysis misc Organic chemistry Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy
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topic_title	QD241-441 Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy ribosome assembly small ribosomal subunit bottom-up proteomics cryo-em single particle analysis
topic	misc QD241-441 misc ribosome assembly misc small ribosomal subunit misc bottom-up proteomics misc cryo-em misc single particle analysis misc Organic chemistry
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title	Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy
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title_full	Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy
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title_sort	good vibrations: structural remodeling of maturing yeast pre-40s ribosomal particles followed by cryo-electron microscopy
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title_auth	Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy
abstract	Assembly of eukaryotic ribosomal subunits is a very complex and sequential process that starts in the nucleolus and finishes in the cytoplasm with the formation of functional ribosomes. Over the past few years, characterization of the many molecular events underlying eukaryotic ribosome biogenesis has been drastically improved by the “resolution revolution” of cryo-electron microscopy (cryo-EM). However, if very early maturation events have been well characterized for both yeast ribosomal subunits, little is known regarding the final maturation steps occurring to the small (40S) ribosomal subunit. To try to bridge this gap, we have used proteomics together with cryo-EM and single particle analysis to characterize yeast pre-40S particles containing the ribosome biogenesis factor Tsr1. Our analyses lead us to refine the timing of the early pre-40S particle maturation steps. Furthermore, we suggest that after an early and structurally stable stage, the beak and platform domains of pre-40S particles enter a “vibrating” or “wriggling” stage, that might be involved in the final maturation of 18S rRNA as well as the fitting of late ribosomal proteins into their mature position.
abstractGer	Assembly of eukaryotic ribosomal subunits is a very complex and sequential process that starts in the nucleolus and finishes in the cytoplasm with the formation of functional ribosomes. Over the past few years, characterization of the many molecular events underlying eukaryotic ribosome biogenesis has been drastically improved by the “resolution revolution” of cryo-electron microscopy (cryo-EM). However, if very early maturation events have been well characterized for both yeast ribosomal subunits, little is known regarding the final maturation steps occurring to the small (40S) ribosomal subunit. To try to bridge this gap, we have used proteomics together with cryo-EM and single particle analysis to characterize yeast pre-40S particles containing the ribosome biogenesis factor Tsr1. Our analyses lead us to refine the timing of the early pre-40S particle maturation steps. Furthermore, we suggest that after an early and structurally stable stage, the beak and platform domains of pre-40S particles enter a “vibrating” or “wriggling” stage, that might be involved in the final maturation of 18S rRNA as well as the fitting of late ribosomal proteins into their mature position.
abstract_unstemmed	Assembly of eukaryotic ribosomal subunits is a very complex and sequential process that starts in the nucleolus and finishes in the cytoplasm with the formation of functional ribosomes. Over the past few years, characterization of the many molecular events underlying eukaryotic ribosome biogenesis has been drastically improved by the “resolution revolution” of cryo-electron microscopy (cryo-EM). However, if very early maturation events have been well characterized for both yeast ribosomal subunits, little is known regarding the final maturation steps occurring to the small (40S) ribosomal subunit. To try to bridge this gap, we have used proteomics together with cryo-EM and single particle analysis to characterize yeast pre-40S particles containing the ribosome biogenesis factor Tsr1. Our analyses lead us to refine the timing of the early pre-40S particle maturation steps. Furthermore, we suggest that after an early and structurally stable stage, the beak and platform domains of pre-40S particles enter a “vibrating” or “wriggling” stage, that might be involved in the final maturation of 18S rRNA as well as the fitting of late ribosomal proteins into their mature position.
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title_short	Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy
url	https://doi.org/10.3390/molecules25051125 https://doaj.org/article/c76148e046f248f78e743d2dd4fc06e8 https://www.mdpi.com/1420-3049/25/5/1125 https://doaj.org/toc/1420-3049
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Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy

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