Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension

Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promo...
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Autor*in:	Ching-Wen Li [verfasserIn] Brett Davis [verfasserIn] Jill Shea [verfasserIn] Himanshu Sant [verfasserIn] Bruce Kent Gale [verfasserIn] Jayant Agarwal [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration

Übergeordnetes Werk:	In: Neural Regeneration Research - Wolters Kluwer Medknow Publications, 2014, 13(2018), 1, Seite 105-111
Übergeordnetes Werk:	volume:13 ; year:2018 ; number:1 ; pages:105-111

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4103/1673-5374.224377

Katalog-ID:	DOAJ041091701

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520			\|a Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
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allfields	10.4103/1673-5374.224377 doi (DE-627)DOAJ041091701 (DE-599)DOAJ802932db862b41d7b8e72f965514440f DE-627 ger DE-627 rakwb eng RC346-429 Ching-Wen Li verfasserin aut Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system Brett Davis verfasserin aut Jill Shea verfasserin aut Himanshu Sant verfasserin aut Bruce Kent Gale verfasserin aut Jayant Agarwal verfasserin aut In Neural Regeneration Research Wolters Kluwer Medknow Publications, 2014 13(2018), 1, Seite 105-111 (DE-627)545785499 (DE-600)2388460-5 18767958 nnns volume:13 year:2018 number:1 pages:105-111 https://doi.org/10.4103/1673-5374.224377 kostenfrei https://doaj.org/article/802932db862b41d7b8e72f965514440f kostenfrei http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li kostenfrei https://doaj.org/toc/1673-5374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 1 105-111
spelling	10.4103/1673-5374.224377 doi (DE-627)DOAJ041091701 (DE-599)DOAJ802932db862b41d7b8e72f965514440f DE-627 ger DE-627 rakwb eng RC346-429 Ching-Wen Li verfasserin aut Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system Brett Davis verfasserin aut Jill Shea verfasserin aut Himanshu Sant verfasserin aut Bruce Kent Gale verfasserin aut Jayant Agarwal verfasserin aut In Neural Regeneration Research Wolters Kluwer Medknow Publications, 2014 13(2018), 1, Seite 105-111 (DE-627)545785499 (DE-600)2388460-5 18767958 nnns volume:13 year:2018 number:1 pages:105-111 https://doi.org/10.4103/1673-5374.224377 kostenfrei https://doaj.org/article/802932db862b41d7b8e72f965514440f kostenfrei http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li kostenfrei https://doaj.org/toc/1673-5374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 1 105-111
allfields_unstemmed	10.4103/1673-5374.224377 doi (DE-627)DOAJ041091701 (DE-599)DOAJ802932db862b41d7b8e72f965514440f DE-627 ger DE-627 rakwb eng RC346-429 Ching-Wen Li verfasserin aut Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system Brett Davis verfasserin aut Jill Shea verfasserin aut Himanshu Sant verfasserin aut Bruce Kent Gale verfasserin aut Jayant Agarwal verfasserin aut In Neural Regeneration Research Wolters Kluwer Medknow Publications, 2014 13(2018), 1, Seite 105-111 (DE-627)545785499 (DE-600)2388460-5 18767958 nnns volume:13 year:2018 number:1 pages:105-111 https://doi.org/10.4103/1673-5374.224377 kostenfrei https://doaj.org/article/802932db862b41d7b8e72f965514440f kostenfrei http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li kostenfrei https://doaj.org/toc/1673-5374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 1 105-111
allfieldsGer	10.4103/1673-5374.224377 doi (DE-627)DOAJ041091701 (DE-599)DOAJ802932db862b41d7b8e72f965514440f DE-627 ger DE-627 rakwb eng RC346-429 Ching-Wen Li verfasserin aut Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system Brett Davis verfasserin aut Jill Shea verfasserin aut Himanshu Sant verfasserin aut Bruce Kent Gale verfasserin aut Jayant Agarwal verfasserin aut In Neural Regeneration Research Wolters Kluwer Medknow Publications, 2014 13(2018), 1, Seite 105-111 (DE-627)545785499 (DE-600)2388460-5 18767958 nnns volume:13 year:2018 number:1 pages:105-111 https://doi.org/10.4103/1673-5374.224377 kostenfrei https://doaj.org/article/802932db862b41d7b8e72f965514440f kostenfrei http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li kostenfrei https://doaj.org/toc/1673-5374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 1 105-111
allfieldsSound	10.4103/1673-5374.224377 doi (DE-627)DOAJ041091701 (DE-599)DOAJ802932db862b41d7b8e72f965514440f DE-627 ger DE-627 rakwb eng RC346-429 Ching-Wen Li verfasserin aut Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system Brett Davis verfasserin aut Jill Shea verfasserin aut Himanshu Sant verfasserin aut Bruce Kent Gale verfasserin aut Jayant Agarwal verfasserin aut In Neural Regeneration Research Wolters Kluwer Medknow Publications, 2014 13(2018), 1, Seite 105-111 (DE-627)545785499 (DE-600)2388460-5 18767958 nnns volume:13 year:2018 number:1 pages:105-111 https://doi.org/10.4103/1673-5374.224377 kostenfrei https://doaj.org/article/802932db862b41d7b8e72f965514440f kostenfrei http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li kostenfrei https://doaj.org/toc/1673-5374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 1 105-111
language	English
source	In Neural Regeneration Research 13(2018), 1, Seite 105-111 volume:13 year:2018 number:1 pages:105-111
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format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration Neurology. Diseases of the nervous system
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container_title	Neural Regeneration Research
authorswithroles_txt_mv	Ching-Wen Li @@aut@@ Brett Davis @@aut@@ Jill Shea @@aut@@ Himanshu Sant @@aut@@ Bruce Kent Gale @@aut@@ Jayant Agarwal @@aut@@
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callnumber-first	R - Medicine
author	Ching-Wen Li
spellingShingle	Ching-Wen Li misc RC346-429 misc nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration misc Neurology. Diseases of the nervous system Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
authorStr	Ching-Wen Li
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topic_title	RC346-429 Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration
topic	misc RC346-429 misc nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration misc Neurology. Diseases of the nervous system
topic_unstemmed	misc RC346-429 misc nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration misc Neurology. Diseases of the nervous system
topic_browse	misc RC346-429 misc nerve regeneration; nerve repair; neural cell migration; neural cell alignment; micropattern; dorsal root ganglion; topological cues; neural regeneration misc Neurology. Diseases of the nervous system
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title	Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
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title_full	Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
author_sort	Ching-Wen Li
journal	Neural Regeneration Research
journalStr	Neural Regeneration Research
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container_start_page	105
author_browse	Ching-Wen Li Brett Davis Jill Shea Himanshu Sant Bruce Kent Gale Jayant Agarwal
container_volume	13
class	RC346-429
format_se	Elektronische Aufsätze
author-letter	Ching-Wen Li
doi_str_mv	10.4103/1673-5374.224377
author2-role	verfasserin
title_sort	optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
callnumber	RC346-429
title_auth	Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
abstract	Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
abstractGer	Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
abstract_unstemmed	Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
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title_short	Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension
url	https://doi.org/10.4103/1673-5374.224377 https://doaj.org/article/802932db862b41d7b8e72f965514440f http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=1;spage=105;epage=111;aulast=Li https://doaj.org/toc/1673-5374
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author2	Brett Davis Jill Shea Himanshu Sant Bruce Kent Gale Jayant Agarwal
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up_date	2024-07-03T18:22:16.621Z
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