Serial macromolecular crystallography at ALBA Synchrotron Light Source
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiment...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Jose M. Martin-Garcia [verfasserIn] Sabine Botha [verfasserIn] Hao Hu [verfasserIn] Rebecca Jernigan [verfasserIn] Albert Castellví [verfasserIn] Stella Lisova [verfasserIn] Fernando Gil [verfasserIn] Barbara Calisto [verfasserIn] Isidro Crespo [verfasserIn] Shatabdi Roy-Chowdhury [verfasserIn] Alice Grieco [verfasserIn] Gihan Ketawala [verfasserIn] Uwe Weierstall [verfasserIn] John Spence [verfasserIn] Petra Fromme [verfasserIn] Nadia Zatsepin [verfasserIn] Dirk Roeland Boer [verfasserIn] Xavi Carpena [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Synchrotron Radiation - International Union of Crystallography, 2022, 29(2022), 3, Seite 896-907 |
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| Übergeordnetes Werk: |
volume:29 ; year:2022 ; number:3 ; pages:896-907 |
| Links: |
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| DOI / URN: |
10.1107/S1600577522002508 |
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| Katalog-ID: |
DOAJ041124294 |
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| 520 | |a The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. | ||
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| 653 | 0 | |a Nuclear and particle physics. Atomic energy. Radioactivity | |
| 653 | 0 | |a Crystallography | |
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| 700 | 0 | |a Hao Hu |e verfasserin |4 aut | |
| 700 | 0 | |a Rebecca Jernigan |e verfasserin |4 aut | |
| 700 | 0 | |a Albert Castellví |e verfasserin |4 aut | |
| 700 | 0 | |a Stella Lisova |e verfasserin |4 aut | |
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| 700 | 0 | |a Dirk Roeland Boer |e verfasserin |4 aut | |
| 700 | 0 | |a Xavi Carpena |e verfasserin |4 aut | |
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10.1107/S1600577522002508 doi (DE-627)DOAJ041124294 (DE-599)DOAJbd920fee84a048b9b7eb3823938dba7e DE-627 ger DE-627 rakwb eng QC770-798 QD901-999 Jose M. Martin-Garcia verfasserin aut Serial macromolecular crystallography at ALBA Synchrotron Light Source 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. serial synchrotron crystallography viscous jet lcp microcrystal alba xaloc Nuclear and particle physics. Atomic energy. Radioactivity Crystallography Sabine Botha verfasserin aut Hao Hu verfasserin aut Rebecca Jernigan verfasserin aut Albert Castellví verfasserin aut Stella Lisova verfasserin aut Fernando Gil verfasserin aut Barbara Calisto verfasserin aut Isidro Crespo verfasserin aut Shatabdi Roy-Chowdhury verfasserin aut Alice Grieco verfasserin aut Gihan Ketawala verfasserin aut Uwe Weierstall verfasserin aut John Spence verfasserin aut Petra Fromme verfasserin aut Nadia Zatsepin verfasserin aut Dirk Roeland Boer verfasserin aut Xavi Carpena verfasserin aut In Journal of Synchrotron Radiation International Union of Crystallography, 2022 29(2022), 3, Seite 896-907 (DE-627)317885510 (DE-600)2021413-3 16005775 nnns volume:29 year:2022 number:3 pages:896-907 https://doi.org/10.1107/S1600577522002508 kostenfrei https://doaj.org/article/bd920fee84a048b9b7eb3823938dba7e kostenfrei http://scripts.iucr.org/cgi-bin/paper?S1600577522002508 kostenfrei https://doaj.org/toc/1600-5775 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 29 2022 3 896-907 |
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10.1107/S1600577522002508 doi (DE-627)DOAJ041124294 (DE-599)DOAJbd920fee84a048b9b7eb3823938dba7e DE-627 ger DE-627 rakwb eng QC770-798 QD901-999 Jose M. Martin-Garcia verfasserin aut Serial macromolecular crystallography at ALBA Synchrotron Light Source 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. serial synchrotron crystallography viscous jet lcp microcrystal alba xaloc Nuclear and particle physics. Atomic energy. Radioactivity Crystallography Sabine Botha verfasserin aut Hao Hu verfasserin aut Rebecca Jernigan verfasserin aut Albert Castellví verfasserin aut Stella Lisova verfasserin aut Fernando Gil verfasserin aut Barbara Calisto verfasserin aut Isidro Crespo verfasserin aut Shatabdi Roy-Chowdhury verfasserin aut Alice Grieco verfasserin aut Gihan Ketawala verfasserin aut Uwe Weierstall verfasserin aut John Spence verfasserin aut Petra Fromme verfasserin aut Nadia Zatsepin verfasserin aut Dirk Roeland Boer verfasserin aut Xavi Carpena verfasserin aut In Journal of Synchrotron Radiation International Union of Crystallography, 2022 29(2022), 3, Seite 896-907 (DE-627)317885510 (DE-600)2021413-3 16005775 nnns volume:29 year:2022 number:3 pages:896-907 https://doi.org/10.1107/S1600577522002508 kostenfrei https://doaj.org/article/bd920fee84a048b9b7eb3823938dba7e kostenfrei http://scripts.iucr.org/cgi-bin/paper?S1600577522002508 kostenfrei https://doaj.org/toc/1600-5775 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 29 2022 3 896-907 |
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10.1107/S1600577522002508 doi (DE-627)DOAJ041124294 (DE-599)DOAJbd920fee84a048b9b7eb3823938dba7e DE-627 ger DE-627 rakwb eng QC770-798 QD901-999 Jose M. Martin-Garcia verfasserin aut Serial macromolecular crystallography at ALBA Synchrotron Light Source 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. serial synchrotron crystallography viscous jet lcp microcrystal alba xaloc Nuclear and particle physics. Atomic energy. Radioactivity Crystallography Sabine Botha verfasserin aut Hao Hu verfasserin aut Rebecca Jernigan verfasserin aut Albert Castellví verfasserin aut Stella Lisova verfasserin aut Fernando Gil verfasserin aut Barbara Calisto verfasserin aut Isidro Crespo verfasserin aut Shatabdi Roy-Chowdhury verfasserin aut Alice Grieco verfasserin aut Gihan Ketawala verfasserin aut Uwe Weierstall verfasserin aut John Spence verfasserin aut Petra Fromme verfasserin aut Nadia Zatsepin verfasserin aut Dirk Roeland Boer verfasserin aut Xavi Carpena verfasserin aut In Journal of Synchrotron Radiation International Union of Crystallography, 2022 29(2022), 3, Seite 896-907 (DE-627)317885510 (DE-600)2021413-3 16005775 nnns volume:29 year:2022 number:3 pages:896-907 https://doi.org/10.1107/S1600577522002508 kostenfrei https://doaj.org/article/bd920fee84a048b9b7eb3823938dba7e kostenfrei http://scripts.iucr.org/cgi-bin/paper?S1600577522002508 kostenfrei https://doaj.org/toc/1600-5775 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 29 2022 3 896-907 |
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10.1107/S1600577522002508 doi (DE-627)DOAJ041124294 (DE-599)DOAJbd920fee84a048b9b7eb3823938dba7e DE-627 ger DE-627 rakwb eng QC770-798 QD901-999 Jose M. Martin-Garcia verfasserin aut Serial macromolecular crystallography at ALBA Synchrotron Light Source 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. serial synchrotron crystallography viscous jet lcp microcrystal alba xaloc Nuclear and particle physics. Atomic energy. Radioactivity Crystallography Sabine Botha verfasserin aut Hao Hu verfasserin aut Rebecca Jernigan verfasserin aut Albert Castellví verfasserin aut Stella Lisova verfasserin aut Fernando Gil verfasserin aut Barbara Calisto verfasserin aut Isidro Crespo verfasserin aut Shatabdi Roy-Chowdhury verfasserin aut Alice Grieco verfasserin aut Gihan Ketawala verfasserin aut Uwe Weierstall verfasserin aut John Spence verfasserin aut Petra Fromme verfasserin aut Nadia Zatsepin verfasserin aut Dirk Roeland Boer verfasserin aut Xavi Carpena verfasserin aut In Journal of Synchrotron Radiation International Union of Crystallography, 2022 29(2022), 3, Seite 896-907 (DE-627)317885510 (DE-600)2021413-3 16005775 nnns volume:29 year:2022 number:3 pages:896-907 https://doi.org/10.1107/S1600577522002508 kostenfrei https://doaj.org/article/bd920fee84a048b9b7eb3823938dba7e kostenfrei http://scripts.iucr.org/cgi-bin/paper?S1600577522002508 kostenfrei https://doaj.org/toc/1600-5775 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 29 2022 3 896-907 |
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Jose M. Martin-Garcia @@aut@@ Sabine Botha @@aut@@ Hao Hu @@aut@@ Rebecca Jernigan @@aut@@ Albert Castellví @@aut@@ Stella Lisova @@aut@@ Fernando Gil @@aut@@ Barbara Calisto @@aut@@ Isidro Crespo @@aut@@ Shatabdi Roy-Chowdhury @@aut@@ Alice Grieco @@aut@@ Gihan Ketawala @@aut@@ Uwe Weierstall @@aut@@ John Spence @@aut@@ Petra Fromme @@aut@@ Nadia Zatsepin @@aut@@ Dirk Roeland Boer @@aut@@ Xavi Carpena @@aut@@ |
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Serial macromolecular crystallography at ALBA Synchrotron Light Source |
| abstract |
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. |
| abstractGer |
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. |
| abstract_unstemmed |
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures. |
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| container_issue |
3 |
| title_short |
Serial macromolecular crystallography at ALBA Synchrotron Light Source |
| url |
https://doi.org/10.1107/S1600577522002508 https://doaj.org/article/bd920fee84a048b9b7eb3823938dba7e http://scripts.iucr.org/cgi-bin/paper?S1600577522002508 https://doaj.org/toc/1600-5775 |
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| author2 |
Sabine Botha Hao Hu Rebecca Jernigan Albert Castellví Stella Lisova Fernando Gil Barbara Calisto Isidro Crespo Shatabdi Roy-Chowdhury Alice Grieco Gihan Ketawala Uwe Weierstall John Spence Petra Fromme Nadia Zatsepin Dirk Roeland Boer Xavi Carpena |
| author2Str |
Sabine Botha Hao Hu Rebecca Jernigan Albert Castellví Stella Lisova Fernando Gil Barbara Calisto Isidro Crespo Shatabdi Roy-Chowdhury Alice Grieco Gihan Ketawala Uwe Weierstall John Spence Petra Fromme Nadia Zatsepin Dirk Roeland Boer Xavi Carpena |
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QC - Physics |
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| doi_str |
10.1107/S1600577522002508 |
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QC770-798 |
| up_date |
2024-07-03T18:32:49.670Z |
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1803583823212969984 |
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| score |
7.3984118 |