Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.

Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from hu...
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Autor*in:	Laura Perin [verfasserIn] Sargis Sedrakyan [verfasserIn] Stefano Giuliani [verfasserIn] Stefano Da Sacco [verfasserIn] Gianni Carraro [verfasserIn] Liron Shiri [verfasserIn] Kevin V Lemley [verfasserIn] Michael Rosol [verfasserIn] Sam Wu [verfasserIn] Anthony Atala [verfasserIn] David Warburton [verfasserIn] Roger E De Filippo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: PLoS ONE - Public Library of Science (PLoS), 2007, 5(2010), 2, p e9357
Übergeordnetes Werk:	volume:5 ; year:2010 ; number:2, p e9357

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1371/journal.pone.0009357

Katalog-ID:	DOAJ041184947

Internformat


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520			\|a Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.
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publishDate	2010
allfields	10.1371/journal.pone.0009357 doi (DE-627)DOAJ041184947 (DE-599)DOAJ7e6d4c68565a4a6e9578ee83bdb989b1 DE-627 ger DE-627 rakwb eng Laura Perin verfasserin aut Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN. Medicine R Science Q Sargis Sedrakyan verfasserin aut Stefano Giuliani verfasserin aut Stefano Da Sacco verfasserin aut Gianni Carraro verfasserin aut Liron Shiri verfasserin aut Kevin V Lemley verfasserin aut Michael Rosol verfasserin aut Sam Wu verfasserin aut Anthony Atala verfasserin aut David Warburton verfasserin aut Roger E De Filippo verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 5(2010), 2, p e9357 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:5 year:2010 number:2, p e9357 https://doi.org/10.1371/journal.pone.0009357 kostenfrei https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 kostenfrei http://europepmc.org/articles/PMC2827539?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010 2, p e9357
spelling	10.1371/journal.pone.0009357 doi (DE-627)DOAJ041184947 (DE-599)DOAJ7e6d4c68565a4a6e9578ee83bdb989b1 DE-627 ger DE-627 rakwb eng Laura Perin verfasserin aut Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN. Medicine R Science Q Sargis Sedrakyan verfasserin aut Stefano Giuliani verfasserin aut Stefano Da Sacco verfasserin aut Gianni Carraro verfasserin aut Liron Shiri verfasserin aut Kevin V Lemley verfasserin aut Michael Rosol verfasserin aut Sam Wu verfasserin aut Anthony Atala verfasserin aut David Warburton verfasserin aut Roger E De Filippo verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 5(2010), 2, p e9357 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:5 year:2010 number:2, p e9357 https://doi.org/10.1371/journal.pone.0009357 kostenfrei https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 kostenfrei http://europepmc.org/articles/PMC2827539?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010 2, p e9357
allfields_unstemmed	10.1371/journal.pone.0009357 doi (DE-627)DOAJ041184947 (DE-599)DOAJ7e6d4c68565a4a6e9578ee83bdb989b1 DE-627 ger DE-627 rakwb eng Laura Perin verfasserin aut Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN. Medicine R Science Q Sargis Sedrakyan verfasserin aut Stefano Giuliani verfasserin aut Stefano Da Sacco verfasserin aut Gianni Carraro verfasserin aut Liron Shiri verfasserin aut Kevin V Lemley verfasserin aut Michael Rosol verfasserin aut Sam Wu verfasserin aut Anthony Atala verfasserin aut David Warburton verfasserin aut Roger E De Filippo verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 5(2010), 2, p e9357 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:5 year:2010 number:2, p e9357 https://doi.org/10.1371/journal.pone.0009357 kostenfrei https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 kostenfrei http://europepmc.org/articles/PMC2827539?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010 2, p e9357
allfieldsGer	10.1371/journal.pone.0009357 doi (DE-627)DOAJ041184947 (DE-599)DOAJ7e6d4c68565a4a6e9578ee83bdb989b1 DE-627 ger DE-627 rakwb eng Laura Perin verfasserin aut Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN. Medicine R Science Q Sargis Sedrakyan verfasserin aut Stefano Giuliani verfasserin aut Stefano Da Sacco verfasserin aut Gianni Carraro verfasserin aut Liron Shiri verfasserin aut Kevin V Lemley verfasserin aut Michael Rosol verfasserin aut Sam Wu verfasserin aut Anthony Atala verfasserin aut David Warburton verfasserin aut Roger E De Filippo verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 5(2010), 2, p e9357 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:5 year:2010 number:2, p e9357 https://doi.org/10.1371/journal.pone.0009357 kostenfrei https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 kostenfrei http://europepmc.org/articles/PMC2827539?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010 2, p e9357
allfieldsSound	10.1371/journal.pone.0009357 doi (DE-627)DOAJ041184947 (DE-599)DOAJ7e6d4c68565a4a6e9578ee83bdb989b1 DE-627 ger DE-627 rakwb eng Laura Perin verfasserin aut Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN. Medicine R Science Q Sargis Sedrakyan verfasserin aut Stefano Giuliani verfasserin aut Stefano Da Sacco verfasserin aut Gianni Carraro verfasserin aut Liron Shiri verfasserin aut Kevin V Lemley verfasserin aut Michael Rosol verfasserin aut Sam Wu verfasserin aut Anthony Atala verfasserin aut David Warburton verfasserin aut Roger E De Filippo verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 5(2010), 2, p e9357 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:5 year:2010 number:2, p e9357 https://doi.org/10.1371/journal.pone.0009357 kostenfrei https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 kostenfrei http://europepmc.org/articles/PMC2827539?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010 2, p e9357
language	English
source	In PLoS ONE 5(2010), 2, p e9357 volume:5 year:2010 number:2, p e9357
sourceStr	In PLoS ONE 5(2010), 2, p e9357 volume:5 year:2010 number:2, p e9357
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container_title	PLoS ONE
authorswithroles_txt_mv	Laura Perin @@aut@@ Sargis Sedrakyan @@aut@@ Stefano Giuliani @@aut@@ Stefano Da Sacco @@aut@@ Gianni Carraro @@aut@@ Liron Shiri @@aut@@ Kevin V Lemley @@aut@@ Michael Rosol @@aut@@ Sam Wu @@aut@@ Anthony Atala @@aut@@ David Warburton @@aut@@ Roger E De Filippo @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
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id	DOAJ041184947
language_de	englisch
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author	Laura Perin
spellingShingle	Laura Perin misc Medicine misc R misc Science misc Q Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
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topic_title	Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis
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title	Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
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title_full	Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis
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author_browse	Laura Perin Sargis Sedrakyan Stefano Giuliani Stefano Da Sacco Gianni Carraro Liron Shiri Kevin V Lemley Michael Rosol Sam Wu Anthony Atala David Warburton Roger E De Filippo
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title_sort	protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis
title_auth	Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
abstract	Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.
abstractGer	Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.
abstract_unstemmed	Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.
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title_short	Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
url	https://doi.org/10.1371/journal.pone.0009357 https://doaj.org/article/7e6d4c68565a4a6e9578ee83bdb989b1 http://europepmc.org/articles/PMC2827539?pdf=render https://doaj.org/toc/1932-6203
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Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.

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