Monoliths in Bioprocess Technology

Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well...
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Gespeichert in:

Autor*in:	Vignesh Rajamanickam [verfasserIn] Christoph Herwig [verfasserIn] Oliver Spadiut [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	monolith pDNA purification virus purification protein purification commercially available monolith

Übergeordnetes Werk:	In: Chromatography - MDPI AG, 2014, 2(2015), 2, Seite 195-212
Übergeordnetes Werk:	volume:2 ; year:2015 ; number:2 ; pages:195-212

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/chromatography2020195

Katalog-ID:	DOAJ041494318

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allfieldsSound	10.3390/chromatography2020195 doi (DE-627)DOAJ041494318 (DE-599)DOAJ517aaef573c444429629e7ac9a7ce4c7 DE-627 ger DE-627 rakwb eng QD1-999 Vignesh Rajamanickam verfasserin aut Monoliths in Bioprocess Technology 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a ‘yellow pages’ for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths. monolith pDNA purification virus purification protein purification commercially available monolith Chemistry Christoph Herwig verfasserin aut Oliver Spadiut verfasserin aut In Chromatography MDPI AG, 2014 2(2015), 2, Seite 195-212 (DE-627)768576156 (DE-600)2733688-8 22279075 nnns volume:2 year:2015 number:2 pages:195-212 https://doi.org/10.3390/chromatography2020195 kostenfrei https://doaj.org/article/517aaef573c444429629e7ac9a7ce4c7 kostenfrei http://www.mdpi.com/2227-9075/2/2/195 kostenfrei https://doaj.org/toc/2227-9075 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 2 195-212
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authorswithroles_txt_mv	Vignesh Rajamanickam @@aut@@ Christoph Herwig @@aut@@ Oliver Spadiut @@aut@@
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author	Vignesh Rajamanickam
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topic_title	QD1-999 Monoliths in Bioprocess Technology monolith pDNA purification virus purification protein purification commercially available monolith
topic	misc QD1-999 misc monolith misc pDNA purification misc virus purification misc protein purification misc commercially available monolith misc Chemistry
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title	Monoliths in Bioprocess Technology
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title_sort	monoliths in bioprocess technology
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title_auth	Monoliths in Bioprocess Technology
abstract	Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a ‘yellow pages’ for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.
abstractGer	Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a ‘yellow pages’ for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.
abstract_unstemmed	Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a ‘yellow pages’ for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.
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title_short	Monoliths in Bioprocess Technology
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