The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients

Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously propos...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chia-Jung Liao [verfasserIn] Chia-Hsun Hsieh [verfasserIn] Feng-Chun Hung [verfasserIn] Hung-Ming Wang [verfasserIn] Wen-Pin Chou [verfasserIn] Min-Hsien Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	circulating tumor cells (CTCs) spheroid cell culture cell isolation and purification head and neck cancer epithelial-to-mesenchymal transition (EMT) prognosis

Übergeordnetes Werk:	In: Cancers - MDPI AG, 2010, 11(2019), 6, p 783
Übergeordnetes Werk:	volume:11 ; year:2019 ; number:6, p 783

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cancers11060783

Katalog-ID:	DOAJ041529456

Internformat


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allfieldsSound	10.3390/cancers11060783 doi (DE-627)DOAJ041529456 (DE-599)DOAJ86fd3e9d3fea47069dd8dd7edf58fc7f DE-627 ger DE-627 rakwb eng RC254-282 Chia-Jung Liao verfasserin aut The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously proposed a 2-step CTC isolation method integrating a negative selection CTC isolation and subsequent spheroid cell culture. In addition to its ability to isolate CTCs, more importantly, the spheroid cell culture used could serve as a cell culture model mimicking the process of new tumor tissue formation during cancer metastasis. Therefore, it is promising not only to selectively isolate metastasis-relevant CTCs but also to test the potential of cancer metastasis and thus the prognosis of disease. To explore these issues, experiments were performed. The key findings of this study demonstrated that the method was able to harvest both epithelial (E)- and mesenchymal (M)-type CTCs without selection bias. Moreover, both the M-type CTC count and the information obtained from the multidrug resistance-associated protein 2 (MRP2) and MRP5 gene expression analysis of the CTCs isolated via the 2-step CTC isolation method might be able to serve as prognostic factors for progression-free survival in head and neck squamous cell carcinoma. circulating tumor cells (CTCs) spheroid cell culture cell isolation and purification head and neck cancer epithelial-to-mesenchymal transition (EMT) prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Chia-Hsun Hsieh verfasserin aut Feng-Chun Hung verfasserin aut Hung-Ming Wang verfasserin aut Wen-Pin Chou verfasserin aut Min-Hsien Wu verfasserin aut In Cancers MDPI AG, 2010 11(2019), 6, p 783 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:11 year:2019 number:6, p 783 https://doi.org/10.3390/cancers11060783 kostenfrei https://doaj.org/article/86fd3e9d3fea47069dd8dd7edf58fc7f kostenfrei https://www.mdpi.com/2072-6694/11/6/783 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 6, p 783
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topic_facet	circulating tumor cells (CTCs) spheroid cell culture cell isolation and purification head and neck cancer epithelial-to-mesenchymal transition (EMT) prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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callnumber-first	R - Medicine
author	Chia-Jung Liao
spellingShingle	Chia-Jung Liao misc RC254-282 misc circulating tumor cells (CTCs) misc spheroid cell culture misc cell isolation and purification misc head and neck cancer misc epithelial-to-mesenchymal transition (EMT) misc prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients
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topic_title	RC254-282 The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients circulating tumor cells (CTCs) spheroid cell culture cell isolation and purification head and neck cancer epithelial-to-mesenchymal transition (EMT) prognosis
topic	misc RC254-282 misc circulating tumor cells (CTCs) misc spheroid cell culture misc cell isolation and purification misc head and neck cancer misc epithelial-to-mesenchymal transition (EMT) misc prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc circulating tumor cells (CTCs) misc spheroid cell culture misc cell isolation and purification misc head and neck cancer misc epithelial-to-mesenchymal transition (EMT) misc prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc circulating tumor cells (CTCs) misc spheroid cell culture misc cell isolation and purification misc head and neck cancer misc epithelial-to-mesenchymal transition (EMT) misc prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients
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title_full	The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients
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title_sort	integration of a three-dimensional spheroid cell culture operation in a circulating tumor cell (ctc) isolation and purification process: a preliminary study of the clinical significance and prognostic role of the ctcs isolated from the blood samples of head and neck cancer patients
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title_auth	The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients
abstract	Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously proposed a 2-step CTC isolation method integrating a negative selection CTC isolation and subsequent spheroid cell culture. In addition to its ability to isolate CTCs, more importantly, the spheroid cell culture used could serve as a cell culture model mimicking the process of new tumor tissue formation during cancer metastasis. Therefore, it is promising not only to selectively isolate metastasis-relevant CTCs but also to test the potential of cancer metastasis and thus the prognosis of disease. To explore these issues, experiments were performed. The key findings of this study demonstrated that the method was able to harvest both epithelial (E)- and mesenchymal (M)-type CTCs without selection bias. Moreover, both the M-type CTC count and the information obtained from the multidrug resistance-associated protein 2 (MRP2) and MRP5 gene expression analysis of the CTCs isolated via the 2-step CTC isolation method might be able to serve as prognostic factors for progression-free survival in head and neck squamous cell carcinoma.
abstractGer	Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously proposed a 2-step CTC isolation method integrating a negative selection CTC isolation and subsequent spheroid cell culture. In addition to its ability to isolate CTCs, more importantly, the spheroid cell culture used could serve as a cell culture model mimicking the process of new tumor tissue formation during cancer metastasis. Therefore, it is promising not only to selectively isolate metastasis-relevant CTCs but also to test the potential of cancer metastasis and thus the prognosis of disease. To explore these issues, experiments were performed. The key findings of this study demonstrated that the method was able to harvest both epithelial (E)- and mesenchymal (M)-type CTCs without selection bias. Moreover, both the M-type CTC count and the information obtained from the multidrug resistance-associated protein 2 (MRP2) and MRP5 gene expression analysis of the CTCs isolated via the 2-step CTC isolation method might be able to serve as prognostic factors for progression-free survival in head and neck squamous cell carcinoma.
abstract_unstemmed	Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously proposed a 2-step CTC isolation method integrating a negative selection CTC isolation and subsequent spheroid cell culture. In addition to its ability to isolate CTCs, more importantly, the spheroid cell culture used could serve as a cell culture model mimicking the process of new tumor tissue formation during cancer metastasis. Therefore, it is promising not only to selectively isolate metastasis-relevant CTCs but also to test the potential of cancer metastasis and thus the prognosis of disease. To explore these issues, experiments were performed. The key findings of this study demonstrated that the method was able to harvest both epithelial (E)- and mesenchymal (M)-type CTCs without selection bias. Moreover, both the M-type CTC count and the information obtained from the multidrug resistance-associated protein 2 (MRP2) and MRP5 gene expression analysis of the CTCs isolated via the 2-step CTC isolation method might be able to serve as prognostic factors for progression-free survival in head and neck squamous cell carcinoma.
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title_short	The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients
url	https://doi.org/10.3390/cancers11060783 https://doaj.org/article/86fd3e9d3fea47069dd8dd7edf58fc7f https://www.mdpi.com/2072-6694/11/6/783 https://doaj.org/toc/2072-6694
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The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients

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