Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities

Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chunyin Wu [verfasserIn] Wei Wang [verfasserIn] Xiaoqing Wang [verfasserIn] Hamza Shahid [verfasserIn] Yuting Yang [verfasserIn] Yangwen Wang [verfasserIn] Shengkun Wang [verfasserIn] Tijiang Shan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity

Übergeordnetes Werk:	In: Annals of Microbiology - BMC, 2021, 72(2022), 1, Seite 11
Übergeordnetes Werk:	volume:72 ; year:2022 ; number:1 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1186/s13213-022-01676-6

Katalog-ID:	DOAJ041647335

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520			\|a Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries.
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allfields	10.1186/s13213-022-01676-6 doi (DE-627)DOAJ041647335 (DE-599)DOAJe0102da7e07c4f08a8daa2c6126fef86 DE-627 ger DE-627 rakwb eng QR1-502 Chunyin Wu verfasserin aut Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology Wei Wang verfasserin aut Xiaoqing Wang verfasserin aut Hamza Shahid verfasserin aut Yuting Yang verfasserin aut Yangwen Wang verfasserin aut Shengkun Wang verfasserin aut Tijiang Shan verfasserin aut In Annals of Microbiology BMC, 2021 72(2022), 1, Seite 11 (DE-627)385615434 (DE-600)2143009-3 18692044 nnns volume:72 year:2022 number:1 pages:11 https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/article/e0102da7e07c4f08a8daa2c6126fef86 kostenfrei https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/toc/1590-4261 Journal toc kostenfrei https://doaj.org/toc/1869-2044 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 72 2022 1 11
spelling	10.1186/s13213-022-01676-6 doi (DE-627)DOAJ041647335 (DE-599)DOAJe0102da7e07c4f08a8daa2c6126fef86 DE-627 ger DE-627 rakwb eng QR1-502 Chunyin Wu verfasserin aut Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology Wei Wang verfasserin aut Xiaoqing Wang verfasserin aut Hamza Shahid verfasserin aut Yuting Yang verfasserin aut Yangwen Wang verfasserin aut Shengkun Wang verfasserin aut Tijiang Shan verfasserin aut In Annals of Microbiology BMC, 2021 72(2022), 1, Seite 11 (DE-627)385615434 (DE-600)2143009-3 18692044 nnns volume:72 year:2022 number:1 pages:11 https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/article/e0102da7e07c4f08a8daa2c6126fef86 kostenfrei https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/toc/1590-4261 Journal toc kostenfrei https://doaj.org/toc/1869-2044 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 72 2022 1 11
allfields_unstemmed	10.1186/s13213-022-01676-6 doi (DE-627)DOAJ041647335 (DE-599)DOAJe0102da7e07c4f08a8daa2c6126fef86 DE-627 ger DE-627 rakwb eng QR1-502 Chunyin Wu verfasserin aut Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology Wei Wang verfasserin aut Xiaoqing Wang verfasserin aut Hamza Shahid verfasserin aut Yuting Yang verfasserin aut Yangwen Wang verfasserin aut Shengkun Wang verfasserin aut Tijiang Shan verfasserin aut In Annals of Microbiology BMC, 2021 72(2022), 1, Seite 11 (DE-627)385615434 (DE-600)2143009-3 18692044 nnns volume:72 year:2022 number:1 pages:11 https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/article/e0102da7e07c4f08a8daa2c6126fef86 kostenfrei https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/toc/1590-4261 Journal toc kostenfrei https://doaj.org/toc/1869-2044 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 72 2022 1 11
allfieldsGer	10.1186/s13213-022-01676-6 doi (DE-627)DOAJ041647335 (DE-599)DOAJe0102da7e07c4f08a8daa2c6126fef86 DE-627 ger DE-627 rakwb eng QR1-502 Chunyin Wu verfasserin aut Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology Wei Wang verfasserin aut Xiaoqing Wang verfasserin aut Hamza Shahid verfasserin aut Yuting Yang verfasserin aut Yangwen Wang verfasserin aut Shengkun Wang verfasserin aut Tijiang Shan verfasserin aut In Annals of Microbiology BMC, 2021 72(2022), 1, Seite 11 (DE-627)385615434 (DE-600)2143009-3 18692044 nnns volume:72 year:2022 number:1 pages:11 https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/article/e0102da7e07c4f08a8daa2c6126fef86 kostenfrei https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/toc/1590-4261 Journal toc kostenfrei https://doaj.org/toc/1869-2044 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 72 2022 1 11
allfieldsSound	10.1186/s13213-022-01676-6 doi (DE-627)DOAJ041647335 (DE-599)DOAJe0102da7e07c4f08a8daa2c6126fef86 DE-627 ger DE-627 rakwb eng QR1-502 Chunyin Wu verfasserin aut Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries. Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology Wei Wang verfasserin aut Xiaoqing Wang verfasserin aut Hamza Shahid verfasserin aut Yuting Yang verfasserin aut Yangwen Wang verfasserin aut Shengkun Wang verfasserin aut Tijiang Shan verfasserin aut In Annals of Microbiology BMC, 2021 72(2022), 1, Seite 11 (DE-627)385615434 (DE-600)2143009-3 18692044 nnns volume:72 year:2022 number:1 pages:11 https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/article/e0102da7e07c4f08a8daa2c6126fef86 kostenfrei https://doi.org/10.1186/s13213-022-01676-6 kostenfrei https://doaj.org/toc/1590-4261 Journal toc kostenfrei https://doaj.org/toc/1869-2044 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 72 2022 1 11
language	English
source	In Annals of Microbiology 72(2022), 1, Seite 11 volume:72 year:2022 number:1 pages:11
sourceStr	In Annals of Microbiology 72(2022), 1, Seite 11 volume:72 year:2022 number:1 pages:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Balanophora polyandra Griff. Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity Microbiology
isfreeaccess_bool	true
container_title	Annals of Microbiology
authorswithroles_txt_mv	Chunyin Wu @@aut@@ Wei Wang @@aut@@ Xiaoqing Wang @@aut@@ Hamza Shahid @@aut@@ Yuting Yang @@aut@@ Yangwen Wang @@aut@@ Shengkun Wang @@aut@@ Tijiang Shan @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	385615434
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language_de	englisch
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Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. 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callnumber-first	Q - Science
author	Chunyin Wu
spellingShingle	Chunyin Wu misc QR1-502 misc Balanophora polyandra Griff. misc Endophytic fungi misc Secondary metabolites misc Antibacterial activity misc Antioxidant activity misc Microbiology Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
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topic_title	QR1-502 Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities Balanophora polyandra Griff Endophytic fungi Secondary metabolites Antibacterial activity Antioxidant activity
topic	misc QR1-502 misc Balanophora polyandra Griff. misc Endophytic fungi misc Secondary metabolites misc Antibacterial activity misc Antioxidant activity misc Microbiology
topic_unstemmed	misc QR1-502 misc Balanophora polyandra Griff. misc Endophytic fungi misc Secondary metabolites misc Antibacterial activity misc Antioxidant activity misc Microbiology
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title	Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
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title_full	Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
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author_browse	Chunyin Wu Wei Wang Xiaoqing Wang Hamza Shahid Yuting Yang Yangwen Wang Shengkun Wang Tijiang Shan
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title_sort	diversity and communities of culturable endophytic fungi from the root holoparasite balanophora polyandra griff. and their antibacterial and antioxidant activities
callnumber	QR1-502
title_auth	Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
abstract	Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries.
abstractGer	Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries.
abstract_unstemmed	Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel bioactive compounds are being isolated from endophytic fungi. The present study investigated the diversity of culturable endophytic fungi from the roots of holoparasite B. polyandra to explore active strains and metabolites. In addition, the antibacterial and antioxidant activities of 22 strains cultured from B. polyandra were also evaluated. Methods The endophytic fungi were identified according to their colony morphology and ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays and DPPH radical scavenging assays were employed to assess the antibacterial and antioxidant activities of ethyl acetate extracts of the endophytic fungi. Results One hundred and twenty-five endophytic strains were isolated from the roots of B. polyandra, including 70 from female samples and 55 from male samples. Of them, twenty-two distinct isolates representing 15 genera and 22 species based on their ITS-rDNA genomic sequence were successfully identified from female and male samples of B. polyandra. The genus Calonectria was the most prevalent genus, with a CF% of 18.3, followed by the genera Clonostachys and Botryosphaeria, with CF% values of 13.4 and 10.0, respectively. Interestingly, the fungal extracts exhibited broad-spectrum antibacterial activities against gram-positive and gram-negative bacteria, as well as potential antioxidant activities with IC50 values ranging from 0.45 to 6.90 mg/mL. Among them, endophytes Bpf-10 (Diaporthe sp.) and Bpf-11 (Botryosphaeria sp.) showed the strongest biological activities and more abundant secondary metabolites. Conclusions This study reported the diversity of endophytic fungi from the roots of B. polyandra and the antibacterial and antioxidant activities of the crude extracts for the first time. The results revealed that B. polyandra contains diverse culturable endophytic fungi that potentially produce natural antibacterial and antioxidant compounds with great value to the agriculture and pharmaceutical industries.
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title_short	Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities
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Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities

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