Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration

Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to i...
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Autor*in:	Liwen Luo [verfasserIn] Junfeng Gong [verfasserIn] Zhouguang Wang [verfasserIn] Yao Liu [verfasserIn] Jiaming Cao [verfasserIn] Jinghao Qin [verfasserIn] Rui Zuo [verfasserIn] Hongyu Zhang [verfasserIn] Shuai Wang [verfasserIn] Ping Zhao [verfasserIn] Di Yang [verfasserIn] Mengjie Zhang [verfasserIn] Yanqiu Wang [verfasserIn] Junfeng Zhang [verfasserIn] Yue Zhou [verfasserIn] Changqing Li [verfasserIn] Bing Ni [verfasserIn] Zhiqiang Tian [verfasserIn] MingHan Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2

Übergeordnetes Werk:	In: Bioactive Materials - KeAi Communications Co., Ltd., 2017, 15(2022), Seite 29-43
Übergeordnetes Werk:	volume:15 ; year:2022 ; pages:29-43

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.bioactmat.2021.12.007

Katalog-ID:	DOAJ041781937

Internformat


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allfields	10.1016/j.bioactmat.2021.12.007 doi (DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Liwen Luo verfasserin aut Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes. Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General) Junfeng Gong verfasserin aut Zhouguang Wang verfasserin aut Yao Liu verfasserin aut Jiaming Cao verfasserin aut Jinghao Qin verfasserin aut Rui Zuo verfasserin aut Hongyu Zhang verfasserin aut Shuai Wang verfasserin aut Ping Zhao verfasserin aut Di Yang verfasserin aut Mengjie Zhang verfasserin aut Yanqiu Wang verfasserin aut Junfeng Zhang verfasserin aut Yue Zhou verfasserin aut Changqing Li verfasserin aut Bing Ni verfasserin aut Zhiqiang Tian verfasserin aut MingHan Liu verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 15(2022), Seite 29-43 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:15 year:2022 pages:29-43 https://doi.org/10.1016/j.bioactmat.2021.12.007 kostenfrei https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21005715 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 29-43
spelling	10.1016/j.bioactmat.2021.12.007 doi (DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Liwen Luo verfasserin aut Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes. Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General) Junfeng Gong verfasserin aut Zhouguang Wang verfasserin aut Yao Liu verfasserin aut Jiaming Cao verfasserin aut Jinghao Qin verfasserin aut Rui Zuo verfasserin aut Hongyu Zhang verfasserin aut Shuai Wang verfasserin aut Ping Zhao verfasserin aut Di Yang verfasserin aut Mengjie Zhang verfasserin aut Yanqiu Wang verfasserin aut Junfeng Zhang verfasserin aut Yue Zhou verfasserin aut Changqing Li verfasserin aut Bing Ni verfasserin aut Zhiqiang Tian verfasserin aut MingHan Liu verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 15(2022), Seite 29-43 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:15 year:2022 pages:29-43 https://doi.org/10.1016/j.bioactmat.2021.12.007 kostenfrei https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21005715 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 29-43
allfields_unstemmed	10.1016/j.bioactmat.2021.12.007 doi (DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Liwen Luo verfasserin aut Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes. Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General) Junfeng Gong verfasserin aut Zhouguang Wang verfasserin aut Yao Liu verfasserin aut Jiaming Cao verfasserin aut Jinghao Qin verfasserin aut Rui Zuo verfasserin aut Hongyu Zhang verfasserin aut Shuai Wang verfasserin aut Ping Zhao verfasserin aut Di Yang verfasserin aut Mengjie Zhang verfasserin aut Yanqiu Wang verfasserin aut Junfeng Zhang verfasserin aut Yue Zhou verfasserin aut Changqing Li verfasserin aut Bing Ni verfasserin aut Zhiqiang Tian verfasserin aut MingHan Liu verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 15(2022), Seite 29-43 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:15 year:2022 pages:29-43 https://doi.org/10.1016/j.bioactmat.2021.12.007 kostenfrei https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21005715 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 29-43
allfieldsGer	10.1016/j.bioactmat.2021.12.007 doi (DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Liwen Luo verfasserin aut Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes. Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General) Junfeng Gong verfasserin aut Zhouguang Wang verfasserin aut Yao Liu verfasserin aut Jiaming Cao verfasserin aut Jinghao Qin verfasserin aut Rui Zuo verfasserin aut Hongyu Zhang verfasserin aut Shuai Wang verfasserin aut Ping Zhao verfasserin aut Di Yang verfasserin aut Mengjie Zhang verfasserin aut Yanqiu Wang verfasserin aut Junfeng Zhang verfasserin aut Yue Zhou verfasserin aut Changqing Li verfasserin aut Bing Ni verfasserin aut Zhiqiang Tian verfasserin aut MingHan Liu verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 15(2022), Seite 29-43 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:15 year:2022 pages:29-43 https://doi.org/10.1016/j.bioactmat.2021.12.007 kostenfrei https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21005715 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 29-43
allfieldsSound	10.1016/j.bioactmat.2021.12.007 doi (DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Liwen Luo verfasserin aut Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes. Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General) Junfeng Gong verfasserin aut Zhouguang Wang verfasserin aut Yao Liu verfasserin aut Jiaming Cao verfasserin aut Jinghao Qin verfasserin aut Rui Zuo verfasserin aut Hongyu Zhang verfasserin aut Shuai Wang verfasserin aut Ping Zhao verfasserin aut Di Yang verfasserin aut Mengjie Zhang verfasserin aut Yanqiu Wang verfasserin aut Junfeng Zhang verfasserin aut Yue Zhou verfasserin aut Changqing Li verfasserin aut Bing Ni verfasserin aut Zhiqiang Tian verfasserin aut MingHan Liu verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 15(2022), Seite 29-43 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:15 year:2022 pages:29-43 https://doi.org/10.1016/j.bioactmat.2021.12.007 kostenfrei https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21005715 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 29-43
language	English
source	In Bioactive Materials 15(2022), Seite 29-43 volume:15 year:2022 pages:29-43
sourceStr	In Bioactive Materials 15(2022), Seite 29-43 volume:15 year:2022 pages:29-43
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2 Materials of engineering and construction. Mechanics of materials Biology (General)
isfreeaccess_bool	true
container_title	Bioactive Materials
authorswithroles_txt_mv	Liwen Luo @@aut@@ Junfeng Gong @@aut@@ Zhouguang Wang @@aut@@ Yao Liu @@aut@@ Jiaming Cao @@aut@@ Jinghao Qin @@aut@@ Rui Zuo @@aut@@ Hongyu Zhang @@aut@@ Shuai Wang @@aut@@ Ping Zhao @@aut@@ Di Yang @@aut@@ Mengjie Zhang @@aut@@ Yanqiu Wang @@aut@@ Junfeng Zhang @@aut@@ Yue Zhou @@aut@@ Changqing Li @@aut@@ Bing Ni @@aut@@ Zhiqiang Tian @@aut@@ MingHan Liu @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1663654956
id	DOAJ041781937
language_de	englisch
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callnumber-first	T - Technology
author	Liwen Luo
spellingShingle	Liwen Luo misc TA401-492 misc QH301-705.5 misc Intervertebral disc degeneration misc Engineered exosomes misc Hydrogels misc Extracellular matrix of costal cartilage misc Sphingosine kinase 2 misc Materials of engineering and construction. Mechanics of materials misc Biology (General) Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
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topic_title	TA401-492 QH301-705.5 Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration Intervertebral disc degeneration Engineered exosomes Hydrogels Extracellular matrix of costal cartilage Sphingosine kinase 2
topic	misc TA401-492 misc QH301-705.5 misc Intervertebral disc degeneration misc Engineered exosomes misc Hydrogels misc Extracellular matrix of costal cartilage misc Sphingosine kinase 2 misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
topic_unstemmed	misc TA401-492 misc QH301-705.5 misc Intervertebral disc degeneration misc Engineered exosomes misc Hydrogels misc Extracellular matrix of costal cartilage misc Sphingosine kinase 2 misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
topic_browse	misc TA401-492 misc QH301-705.5 misc Intervertebral disc degeneration misc Engineered exosomes misc Hydrogels misc Extracellular matrix of costal cartilage misc Sphingosine kinase 2 misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
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title	Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
ctrlnum	(DE-627)DOAJ041781937 (DE-599)DOAJ6de844ae85df43228508f16ac1e20a74
title_full	Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
author_sort	Liwen Luo
journal	Bioactive Materials
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author_browse	Liwen Luo Junfeng Gong Zhouguang Wang Yao Liu Jiaming Cao Jinghao Qin Rui Zuo Hongyu Zhang Shuai Wang Ping Zhao Di Yang Mengjie Zhang Yanqiu Wang Junfeng Zhang Yue Zhou Changqing Li Bing Ni Zhiqiang Tian MingHan Liu
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format_se	Elektronische Aufsätze
author-letter	Liwen Luo
doi_str_mv	10.1016/j.bioactmat.2021.12.007
author2-role	verfasserin
title_sort	injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
callnumber	TA401-492
title_auth	Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
abstract	Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.
abstractGer	Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.
abstract_unstemmed	Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration
url	https://doi.org/10.1016/j.bioactmat.2021.12.007 https://doaj.org/article/6de844ae85df43228508f16ac1e20a74 http://www.sciencedirect.com/science/article/pii/S2452199X21005715 https://doaj.org/toc/2452-199X
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author2	Junfeng Gong Zhouguang Wang Yao Liu Jiaming Cao Jinghao Qin Rui Zuo Hongyu Zhang Shuai Wang Ping Zhao Di Yang Mengjie Zhang Yanqiu Wang Junfeng Zhang Yue Zhou Changqing Li Bing Ni Zhiqiang Tian MingHan Liu
author2Str	Junfeng Gong Zhouguang Wang Yao Liu Jiaming Cao Jinghao Qin Rui Zuo Hongyu Zhang Shuai Wang Ping Zhao Di Yang Mengjie Zhang Yanqiu Wang Junfeng Zhang Yue Zhou Changqing Li Bing Ni Zhiqiang Tian MingHan Liu
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doi_str	10.1016/j.bioactmat.2021.12.007
callnumber-a	TA401-492
up_date	2024-07-03T22:06:58.298Z
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Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration

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