Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats

Abstract In the present study, free interstitial levels reached by metformin in the liver were investigated in control and diabetic rats by microdialysis. Firstly, a bioanalytical method using an HPLC-UV system to determine the drug concentration in microdialysis samples was validated. The blood glu...
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Gespeichert in:

Autor*in:	Andressa Braga [verfasserIn] Jaqueline Schneider Izolan [verfasserIn] Teresa Cristina Dalla Costa [verfasserIn] Bibiana Verlindo de Araújo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Metformin Microdialysis Pharmacokinetics Experimental diabetes

Übergeordnetes Werk:	In: Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo, 2010, 58(2022)
Übergeordnetes Werk:	volume:58 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/s2175-97902022e19674

Katalog-ID:	DOAJ042177855

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allfieldsSound	10.1590/s2175-97902022e19674 doi (DE-627)DOAJ042177855 (DE-599)DOAJ68a9340d6fe34629a448cc1314f7eb1b DE-627 ger DE-627 rakwb eng RS1-441 Andressa Braga verfasserin aut Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, free interstitial levels reached by metformin in the liver were investigated in control and diabetic rats by microdialysis. Firstly, a bioanalytical method using an HPLC-UV system to determine the drug concentration in microdialysis samples was validated. The blood glucose levels and biochemical parameters were investigated in control and diabetic animals. Following that, both groups received a dose of 50 mg/kg of metformin iv bolus and the free interstitial levels reached in the liver were assessed by microdialysis. The method was validated according to FDA guidelines being suitable to quantify free concentrations of metformin in the liver of control and diabetics rats. Free exposure to metformin was similar in control and diabetic animals: AUC0-∞ 118.50 ± 40.18 vs 112.93 ± 50.25 µg.h/mL, respectively. The half-life in tissue was similar to that described in the literature for plasma. Hence diabetes induced by streptozotocin after administration of nicotinamide in our study did not damage the renal and hepatic function of the animals. The levels reached in the liver were 1.6 times higher than the free plasma concentrations, demonstrating higher liver penetration of metformin. This is the first investigation in liver interstitial concentration of metformin in control and diabetic rats. Metformin Microdialysis Pharmacokinetics Experimental diabetes Pharmacy and materia medica Jaqueline Schneider Izolan verfasserin aut Teresa Cristina Dalla Costa verfasserin aut Bibiana Verlindo de Araújo verfasserin aut In Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo, 2010 58(2022) (DE-627)72781057X (DE-600)2685807-1 21759790 nnns volume:58 year:2022 https://doi.org/10.1590/s2175-97902022e19674 kostenfrei https://doaj.org/article/68a9340d6fe34629a448cc1314f7eb1b kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100601&tlng=en kostenfrei https://doaj.org/toc/2175-9790 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 58 2022
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callnumber-first	R - Medicine
author	Andressa Braga
spellingShingle	Andressa Braga misc RS1-441 misc Metformin misc Microdialysis misc Pharmacokinetics misc Experimental diabetes misc Pharmacy and materia medica Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats
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topic_title	RS1-441 Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats Metformin Microdialysis Pharmacokinetics Experimental diabetes
topic	misc RS1-441 misc Metformin misc Microdialysis misc Pharmacokinetics misc Experimental diabetes misc Pharmacy and materia medica
topic_unstemmed	misc RS1-441 misc Metformin misc Microdialysis misc Pharmacokinetics misc Experimental diabetes misc Pharmacy and materia medica
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title	Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats
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title_full	Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats
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author_browse	Andressa Braga Jaqueline Schneider Izolan Teresa Cristina Dalla Costa Bibiana Verlindo de Araújo
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title_sort	free interstitial levels of metformin in the liver of healthy and diabetic wistar rats
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title_auth	Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats
abstract	Abstract In the present study, free interstitial levels reached by metformin in the liver were investigated in control and diabetic rats by microdialysis. Firstly, a bioanalytical method using an HPLC-UV system to determine the drug concentration in microdialysis samples was validated. The blood glucose levels and biochemical parameters were investigated in control and diabetic animals. Following that, both groups received a dose of 50 mg/kg of metformin iv bolus and the free interstitial levels reached in the liver were assessed by microdialysis. The method was validated according to FDA guidelines being suitable to quantify free concentrations of metformin in the liver of control and diabetics rats. Free exposure to metformin was similar in control and diabetic animals: AUC0-∞ 118.50 ± 40.18 vs 112.93 ± 50.25 µg.h/mL, respectively. The half-life in tissue was similar to that described in the literature for plasma. Hence diabetes induced by streptozotocin after administration of nicotinamide in our study did not damage the renal and hepatic function of the animals. The levels reached in the liver were 1.6 times higher than the free plasma concentrations, demonstrating higher liver penetration of metformin. This is the first investigation in liver interstitial concentration of metformin in control and diabetic rats.
abstractGer	Abstract In the present study, free interstitial levels reached by metformin in the liver were investigated in control and diabetic rats by microdialysis. Firstly, a bioanalytical method using an HPLC-UV system to determine the drug concentration in microdialysis samples was validated. The blood glucose levels and biochemical parameters were investigated in control and diabetic animals. Following that, both groups received a dose of 50 mg/kg of metformin iv bolus and the free interstitial levels reached in the liver were assessed by microdialysis. The method was validated according to FDA guidelines being suitable to quantify free concentrations of metformin in the liver of control and diabetics rats. Free exposure to metformin was similar in control and diabetic animals: AUC0-∞ 118.50 ± 40.18 vs 112.93 ± 50.25 µg.h/mL, respectively. The half-life in tissue was similar to that described in the literature for plasma. Hence diabetes induced by streptozotocin after administration of nicotinamide in our study did not damage the renal and hepatic function of the animals. The levels reached in the liver were 1.6 times higher than the free plasma concentrations, demonstrating higher liver penetration of metformin. This is the first investigation in liver interstitial concentration of metformin in control and diabetic rats.
abstract_unstemmed	Abstract In the present study, free interstitial levels reached by metformin in the liver were investigated in control and diabetic rats by microdialysis. Firstly, a bioanalytical method using an HPLC-UV system to determine the drug concentration in microdialysis samples was validated. The blood glucose levels and biochemical parameters were investigated in control and diabetic animals. Following that, both groups received a dose of 50 mg/kg of metformin iv bolus and the free interstitial levels reached in the liver were assessed by microdialysis. The method was validated according to FDA guidelines being suitable to quantify free concentrations of metformin in the liver of control and diabetics rats. Free exposure to metformin was similar in control and diabetic animals: AUC0-∞ 118.50 ± 40.18 vs 112.93 ± 50.25 µg.h/mL, respectively. The half-life in tissue was similar to that described in the literature for plasma. Hence diabetes induced by streptozotocin after administration of nicotinamide in our study did not damage the renal and hepatic function of the animals. The levels reached in the liver were 1.6 times higher than the free plasma concentrations, demonstrating higher liver penetration of metformin. This is the first investigation in liver interstitial concentration of metformin in control and diabetic rats.
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title_short	Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats
url	https://doi.org/10.1590/s2175-97902022e19674 https://doaj.org/article/68a9340d6fe34629a448cc1314f7eb1b http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100601&tlng=en https://doaj.org/toc/2175-9790
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Free interstitial levels of metformin in the liver of healthy and diabetic Wistar rats

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