Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works...
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Autor*in:	Dugarmaa Ulzii [verfasserIn] Makiko Kido-Nakahara [verfasserIn] Takeshi Nakahara [verfasserIn] Gaku Tsuji [verfasserIn] Kazuhisa Furue [verfasserIn] Akiko Hashimoto-Hachiya [verfasserIn] Masutaka Furue [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 20(2019), 13, p 3324
Übergeordnetes Werk:	volume:20 ; year:2019 ; number:13, p 3324

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms20133324

Katalog-ID:	DOAJ042410304

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520			\|a The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling.
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Indexfelder

author_variant	d u du m k n mkn t n tn g t gt k f kf a h h ahh m f mf
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allfields	10.3390/ijms20133324 doi (DE-627)DOAJ042410304 (DE-599)DOAJ1a244956f4e9463c92037c8069b34d19 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Dugarmaa Ulzii verfasserin aut Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling. scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry Makiko Kido-Nakahara verfasserin aut Takeshi Nakahara verfasserin aut Gaku Tsuji verfasserin aut Kazuhisa Furue verfasserin aut Akiko Hashimoto-Hachiya verfasserin aut Masutaka Furue verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 13, p 3324 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:13, p 3324 https://doi.org/10.3390/ijms20133324 kostenfrei https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 kostenfrei https://www.mdpi.com/1422-0067/20/13/3324 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 13, p 3324
spelling	10.3390/ijms20133324 doi (DE-627)DOAJ042410304 (DE-599)DOAJ1a244956f4e9463c92037c8069b34d19 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Dugarmaa Ulzii verfasserin aut Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling. scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry Makiko Kido-Nakahara verfasserin aut Takeshi Nakahara verfasserin aut Gaku Tsuji verfasserin aut Kazuhisa Furue verfasserin aut Akiko Hashimoto-Hachiya verfasserin aut Masutaka Furue verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 13, p 3324 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:13, p 3324 https://doi.org/10.3390/ijms20133324 kostenfrei https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 kostenfrei https://www.mdpi.com/1422-0067/20/13/3324 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 13, p 3324
allfields_unstemmed	10.3390/ijms20133324 doi (DE-627)DOAJ042410304 (DE-599)DOAJ1a244956f4e9463c92037c8069b34d19 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Dugarmaa Ulzii verfasserin aut Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling. scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry Makiko Kido-Nakahara verfasserin aut Takeshi Nakahara verfasserin aut Gaku Tsuji verfasserin aut Kazuhisa Furue verfasserin aut Akiko Hashimoto-Hachiya verfasserin aut Masutaka Furue verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 13, p 3324 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:13, p 3324 https://doi.org/10.3390/ijms20133324 kostenfrei https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 kostenfrei https://www.mdpi.com/1422-0067/20/13/3324 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 13, p 3324
allfieldsGer	10.3390/ijms20133324 doi (DE-627)DOAJ042410304 (DE-599)DOAJ1a244956f4e9463c92037c8069b34d19 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Dugarmaa Ulzii verfasserin aut Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling. scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry Makiko Kido-Nakahara verfasserin aut Takeshi Nakahara verfasserin aut Gaku Tsuji verfasserin aut Kazuhisa Furue verfasserin aut Akiko Hashimoto-Hachiya verfasserin aut Masutaka Furue verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 13, p 3324 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:13, p 3324 https://doi.org/10.3390/ijms20133324 kostenfrei https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 kostenfrei https://www.mdpi.com/1422-0067/20/13/3324 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 13, p 3324
allfieldsSound	10.3390/ijms20133324 doi (DE-627)DOAJ042410304 (DE-599)DOAJ1a244956f4e9463c92037c8069b34d19 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Dugarmaa Ulzii verfasserin aut Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling. scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry Makiko Kido-Nakahara verfasserin aut Takeshi Nakahara verfasserin aut Gaku Tsuji verfasserin aut Kazuhisa Furue verfasserin aut Akiko Hashimoto-Hachiya verfasserin aut Masutaka Furue verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 13, p 3324 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:13, p 3324 https://doi.org/10.3390/ijms20133324 kostenfrei https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 kostenfrei https://www.mdpi.com/1422-0067/20/13/3324 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 13, p 3324
language	English
source	In International Journal of Molecular Sciences 20(2019), 13, p 3324 volume:20 year:2019 number:13, p 3324
sourceStr	In International Journal of Molecular Sciences 20(2019), 13, p 3324 volume:20 year:2019 number:13, p 3324
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Dugarmaa Ulzii @@aut@@ Makiko Kido-Nakahara @@aut@@ Takeshi Nakahara @@aut@@ Gaku Tsuji @@aut@@ Kazuhisa Furue @@aut@@ Akiko Hashimoto-Hachiya @@aut@@ Masutaka Furue @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ042410304
language_de	englisch
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callnumber-first	Q - Science
author	Dugarmaa Ulzii
spellingShingle	Dugarmaa Ulzii misc QH301-705.5 misc QD1-999 misc scratch injury misc IL-13Rα2 misc keratinocyte misc IL-13 misc atopic dermatitis misc IL-4Rα misc IL-13Rα1 misc involucrin misc Biology (General) misc Chemistry Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes
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topic_title	QH301-705.5 QD1-999 Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes scratch injury IL-13Rα2 keratinocyte IL-13 atopic dermatitis IL-4Rα IL-13Rα1 involucrin
topic	misc QH301-705.5 misc QD1-999 misc scratch injury misc IL-13Rα2 misc keratinocyte misc IL-13 misc atopic dermatitis misc IL-4Rα misc IL-13Rα1 misc involucrin misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc scratch injury misc IL-13Rα2 misc keratinocyte misc IL-13 misc atopic dermatitis misc IL-4Rα misc IL-13Rα1 misc involucrin misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc scratch injury misc IL-13Rα2 misc keratinocyte misc IL-13 misc atopic dermatitis misc IL-4Rα misc IL-13Rα1 misc involucrin misc Biology (General) misc Chemistry
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hierarchy_parent_title	International Journal of Molecular Sciences
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title	Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes
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title_full	Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes
author_sort	Dugarmaa Ulzii
journal	International Journal of Molecular Sciences
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author_browse	Dugarmaa Ulzii Makiko Kido-Nakahara Takeshi Nakahara Gaku Tsuji Kazuhisa Furue Akiko Hashimoto-Hachiya Masutaka Furue
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author-letter	Dugarmaa Ulzii
doi_str_mv	10.3390/ijms20133324
author2-role	verfasserin
title_sort	scratching counteracts il-13 signaling by upregulating the decoy receptor il-13rα2 in keratinocytes
callnumber	QH301-705.5
title_auth	Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes
abstract	The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling.
abstractGer	The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling.
abstract_unstemmed	The vicious itch−scratch cycle is a cardinal feature of atopic dermatitis (AD), in which IL-13 signaling plays a dominant role. Keratinocytes express two receptors: The heterodimeric IL-4Rα/IL-13Rα1 and IL-13Rα2. The former one transduces a functional IL-13 signal, whereas the latter IL-13Rα2 works as a nonfunctional decoy receptor. To examine whether scratch injury affects the expression of IL-4Rα, IL-13Rα1, and IL-13Rα2, we scratched confluent keratinocyte sheets and examined the expression of three IL-13 receptors using quantitative real-time PCR (qRT-PCR) and immunofluorescence techniques. Scratch injuries significantly upregulated the expression of <i<IL13RA2</i< in a scratch line number-dependent manner. Scratch-induced <i<IL13RA2</i< upregulation was synergistically enhanced in the simultaneous presence of IL-13. In contrast, scratch injuries did not alter the expression of <i<IL4R</i< and <i<IL13RA1</i<, even in the presence of IL-13. Scratch-induced <i<IL13RA2</i< expression was dependent on ERK1/2 and p38 MAPK signals. The expression of IL-13Rα2 protein was indeed augmented in the scratch edge area and was also overexpressed in lichenified lesional AD skin. IL-13 inhibited the expression of involucrin, an important epidermal terminal differentiation molecule. IL-13-mediated downregulation of involucrin was attenuated in IL-13Rα2-overexpressed keratinocytes, confirming the decoy function of IL-13Rα2. Our findings indicate that scratching upregulates the expression of the IL-13 decoy receptor IL-13Rα2 and counteracts IL-13 signaling.
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container_issue	13, p 3324
title_short	Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes
url	https://doi.org/10.3390/ijms20133324 https://doaj.org/article/1a244956f4e9463c92037c8069b34d19 https://www.mdpi.com/1422-0067/20/13/3324 https://doaj.org/toc/1422-0067
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Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

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