Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome

Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable r...
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Gespeichert in:

Autor*in:	Christian Büll [verfasserIn] Hiren J. Joshi [verfasserIn] Henrik Clausen [verfasserIn] Yoshiki Narimatsu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 1(2020), 1, Seite 100017-
Übergeordnetes Werk:	volume:1 ; year:2020 ; number:1 ; pages:100017-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2020.100017

Katalog-ID:	DOAJ042499836

Internformat


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allfields	10.1016/j.xpro.2020.100017 doi (DE-627)DOAJ042499836 (DE-599)DOAJ9b5e2ce0ca6d4bb7a70fadbd17df1890 DE-627 ger DE-627 rakwb eng Q1-390 Christian Büll verfasserin aut Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Science (General) Hiren J. Joshi verfasserin aut Henrik Clausen verfasserin aut Yoshiki Narimatsu verfasserin aut In STAR Protocols Elsevier, 2020 1(2020), 1, Seite 100017- (DE-627)1747970557 26661667 nnns volume:1 year:2020 number:1 pages:100017- https://doi.org/10.1016/j.xpro.2020.100017 kostenfrei https://doaj.org/article/9b5e2ce0ca6d4bb7a70fadbd17df1890 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166720300046 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 1 2020 1 100017-
spelling	10.1016/j.xpro.2020.100017 doi (DE-627)DOAJ042499836 (DE-599)DOAJ9b5e2ce0ca6d4bb7a70fadbd17df1890 DE-627 ger DE-627 rakwb eng Q1-390 Christian Büll verfasserin aut Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Science (General) Hiren J. Joshi verfasserin aut Henrik Clausen verfasserin aut Yoshiki Narimatsu verfasserin aut In STAR Protocols Elsevier, 2020 1(2020), 1, Seite 100017- (DE-627)1747970557 26661667 nnns volume:1 year:2020 number:1 pages:100017- https://doi.org/10.1016/j.xpro.2020.100017 kostenfrei https://doaj.org/article/9b5e2ce0ca6d4bb7a70fadbd17df1890 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166720300046 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 1 2020 1 100017-
allfields_unstemmed	10.1016/j.xpro.2020.100017 doi (DE-627)DOAJ042499836 (DE-599)DOAJ9b5e2ce0ca6d4bb7a70fadbd17df1890 DE-627 ger DE-627 rakwb eng Q1-390 Christian Büll verfasserin aut Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Science (General) Hiren J. Joshi verfasserin aut Henrik Clausen verfasserin aut Yoshiki Narimatsu verfasserin aut In STAR Protocols Elsevier, 2020 1(2020), 1, Seite 100017- (DE-627)1747970557 26661667 nnns volume:1 year:2020 number:1 pages:100017- https://doi.org/10.1016/j.xpro.2020.100017 kostenfrei https://doaj.org/article/9b5e2ce0ca6d4bb7a70fadbd17df1890 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166720300046 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 1 2020 1 100017-
allfieldsGer	10.1016/j.xpro.2020.100017 doi (DE-627)DOAJ042499836 (DE-599)DOAJ9b5e2ce0ca6d4bb7a70fadbd17df1890 DE-627 ger DE-627 rakwb eng Q1-390 Christian Büll verfasserin aut Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Science (General) Hiren J. Joshi verfasserin aut Henrik Clausen verfasserin aut Yoshiki Narimatsu verfasserin aut In STAR Protocols Elsevier, 2020 1(2020), 1, Seite 100017- (DE-627)1747970557 26661667 nnns volume:1 year:2020 number:1 pages:100017- https://doi.org/10.1016/j.xpro.2020.100017 kostenfrei https://doaj.org/article/9b5e2ce0ca6d4bb7a70fadbd17df1890 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166720300046 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 1 2020 1 100017-
allfieldsSound	10.1016/j.xpro.2020.100017 doi (DE-627)DOAJ042499836 (DE-599)DOAJ9b5e2ce0ca6d4bb7a70fadbd17df1890 DE-627 ger DE-627 rakwb eng Q1-390 Christian Büll verfasserin aut Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Science (General) Hiren J. Joshi verfasserin aut Henrik Clausen verfasserin aut Yoshiki Narimatsu verfasserin aut In STAR Protocols Elsevier, 2020 1(2020), 1, Seite 100017- (DE-627)1747970557 26661667 nnns volume:1 year:2020 number:1 pages:100017- https://doi.org/10.1016/j.xpro.2020.100017 kostenfrei https://doaj.org/article/9b5e2ce0ca6d4bb7a70fadbd17df1890 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166720300046 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 1 2020 1 100017-
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title_sort	cell-based glycan arrays—a practical guide to dissect the human glycome
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title_auth	Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
abstract	Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
abstractGer	Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
abstract_unstemmed	Summary: Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
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