Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)

Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains l...
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Autor*in:	Mingyi Wang [verfasserIn] Hui Ding [verfasserIn] Shaoxuan Wu [verfasserIn] Mengya Wang [verfasserIn] Cun Wei [verfasserIn] Bo Wang [verfasserIn] Zhenmin Bao [verfasserIn] Jingjie Hu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	teleost leopard coral grouper gonad vasa germ cell marker

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 13(2022), 6, p 1077
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:6, p 1077

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes13061077

Katalog-ID:	DOAJ04268742X

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520			\|a Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.
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allfields	10.3390/genes13061077 doi (DE-627)DOAJ04268742X (DE-599)DOAJ530d6df4f1fc443dbbd44e93a190a1a9 DE-627 ger DE-627 rakwb eng QH426-470 Mingyi Wang verfasserin aut Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells. teleost leopard coral grouper gonad vasa germ cell marker Genetics Hui Ding verfasserin aut Shaoxuan Wu verfasserin aut Mengya Wang verfasserin aut Cun Wei verfasserin aut Bo Wang verfasserin aut Zhenmin Bao verfasserin aut Jingjie Hu verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1077 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1077 https://doi.org/10.3390/genes13061077 kostenfrei https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 kostenfrei https://www.mdpi.com/2073-4425/13/6/1077 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1077
spelling	10.3390/genes13061077 doi (DE-627)DOAJ04268742X (DE-599)DOAJ530d6df4f1fc443dbbd44e93a190a1a9 DE-627 ger DE-627 rakwb eng QH426-470 Mingyi Wang verfasserin aut Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells. teleost leopard coral grouper gonad vasa germ cell marker Genetics Hui Ding verfasserin aut Shaoxuan Wu verfasserin aut Mengya Wang verfasserin aut Cun Wei verfasserin aut Bo Wang verfasserin aut Zhenmin Bao verfasserin aut Jingjie Hu verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1077 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1077 https://doi.org/10.3390/genes13061077 kostenfrei https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 kostenfrei https://www.mdpi.com/2073-4425/13/6/1077 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1077
allfields_unstemmed	10.3390/genes13061077 doi (DE-627)DOAJ04268742X (DE-599)DOAJ530d6df4f1fc443dbbd44e93a190a1a9 DE-627 ger DE-627 rakwb eng QH426-470 Mingyi Wang verfasserin aut Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells. teleost leopard coral grouper gonad vasa germ cell marker Genetics Hui Ding verfasserin aut Shaoxuan Wu verfasserin aut Mengya Wang verfasserin aut Cun Wei verfasserin aut Bo Wang verfasserin aut Zhenmin Bao verfasserin aut Jingjie Hu verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1077 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1077 https://doi.org/10.3390/genes13061077 kostenfrei https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 kostenfrei https://www.mdpi.com/2073-4425/13/6/1077 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1077
allfieldsGer	10.3390/genes13061077 doi (DE-627)DOAJ04268742X (DE-599)DOAJ530d6df4f1fc443dbbd44e93a190a1a9 DE-627 ger DE-627 rakwb eng QH426-470 Mingyi Wang verfasserin aut Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells. teleost leopard coral grouper gonad vasa germ cell marker Genetics Hui Ding verfasserin aut Shaoxuan Wu verfasserin aut Mengya Wang verfasserin aut Cun Wei verfasserin aut Bo Wang verfasserin aut Zhenmin Bao verfasserin aut Jingjie Hu verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1077 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1077 https://doi.org/10.3390/genes13061077 kostenfrei https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 kostenfrei https://www.mdpi.com/2073-4425/13/6/1077 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1077
allfieldsSound	10.3390/genes13061077 doi (DE-627)DOAJ04268742X (DE-599)DOAJ530d6df4f1fc443dbbd44e93a190a1a9 DE-627 ger DE-627 rakwb eng QH426-470 Mingyi Wang verfasserin aut Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells. teleost leopard coral grouper gonad vasa germ cell marker Genetics Hui Ding verfasserin aut Shaoxuan Wu verfasserin aut Mengya Wang verfasserin aut Cun Wei verfasserin aut Bo Wang verfasserin aut Zhenmin Bao verfasserin aut Jingjie Hu verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1077 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1077 https://doi.org/10.3390/genes13061077 kostenfrei https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 kostenfrei https://www.mdpi.com/2073-4425/13/6/1077 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1077
language	English
source	In Genes 13(2022), 6, p 1077 volume:13 year:2022 number:6, p 1077
sourceStr	In Genes 13(2022), 6, p 1077 volume:13 year:2022 number:6, p 1077
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topic_facet	teleost leopard coral grouper gonad vasa germ cell marker Genetics
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container_title	Genes
authorswithroles_txt_mv	Mingyi Wang @@aut@@ Hui Ding @@aut@@ Shaoxuan Wu @@aut@@ Mengya Wang @@aut@@ Cun Wei @@aut@@ Bo Wang @@aut@@ Zhenmin Bao @@aut@@ Jingjie Hu @@aut@@
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callnumber-first	Q - Science
author	Mingyi Wang
spellingShingle	Mingyi Wang misc QH426-470 misc teleost misc leopard coral grouper misc gonad misc vasa misc germ cell marker misc Genetics Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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topic_title	QH426-470 Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<) teleost leopard coral grouper gonad vasa germ cell marker
topic	misc QH426-470 misc teleost misc leopard coral grouper misc gonad misc vasa misc germ cell marker misc Genetics
topic_unstemmed	misc QH426-470 misc teleost misc leopard coral grouper misc gonad misc vasa misc germ cell marker misc Genetics
topic_browse	misc QH426-470 misc teleost misc leopard coral grouper misc gonad misc vasa misc germ cell marker misc Genetics
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title	Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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title_full	Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
author_sort	Mingyi Wang
journal	Genes
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author_browse	Mingyi Wang Hui Ding Shaoxuan Wu Mengya Wang Cun Wei Bo Wang Zhenmin Bao Jingjie Hu
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author-letter	Mingyi Wang
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title_sort	vasa is a potential germ cell marker in leopard coral grouper (<i<plectropomus leopardus</i<)
callnumber	QH426-470
title_auth	Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
abstract	Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.
abstractGer	Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.
abstract_unstemmed	Vasa (<i<Ddx4</i<, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, <i<vasa</i< homolog termed <i<Plvasa</i< was isolated and identified <i<Plvasa</i< as a putative germ cell marker in the leopard coral grouper, (<i<Plectropomus leopardus</i<). Results indicated that <i<Plvasa</i< contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that <i<Plvasa</i< was homologous with other teleosts. Additionally, the expression of <i<Plvasa</i< was significantly higher in gonads than in other tissues in adult individuals (<i<p</i< < 0.05). Further, the distribution of <i<Plvasa</i< revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the <i<Plvasa</i< gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.
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container_issue	6, p 1077
title_short	Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
url	https://doi.org/10.3390/genes13061077 https://doaj.org/article/530d6df4f1fc443dbbd44e93a190a1a9 https://www.mdpi.com/2073-4425/13/6/1077 https://doaj.org/toc/2073-4425
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author2	Hui Ding Shaoxuan Wu Mengya Wang Cun Wei Bo Wang Zhenmin Bao Jingjie Hu
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up_date	2024-07-03T13:30:20.422Z
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Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (<i<Plectropomus leopardus</i<)

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