Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor

Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially on...
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Autor*in:	Katarzyna Marek [verfasserIn] Federico Armando [verfasserIn] Vanessa Maria Nippold [verfasserIn] Karl Rohn [verfasserIn] Philippe Plattet [verfasserIn] Graham Brogden [verfasserIn] Gisa Gerold [verfasserIn] Wolfgang Baumgärtner [verfasserIn] Christina Puff [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 11, p 6156
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:11, p 6156

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23116156

Katalog-ID:	DOAJ042843359

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520			\|a Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies.
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allfields	10.3390/ijms23116156 doi (DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Katarzyna Marek verfasserin aut Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies. canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry Federico Armando verfasserin aut Vanessa Maria Nippold verfasserin aut Karl Rohn verfasserin aut Philippe Plattet verfasserin aut Graham Brogden verfasserin aut Gisa Gerold verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 11, p 6156 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:11, p 6156 https://doi.org/10.3390/ijms23116156 kostenfrei https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 kostenfrei https://www.mdpi.com/1422-0067/23/11/6156 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 11, p 6156
spelling	10.3390/ijms23116156 doi (DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Katarzyna Marek verfasserin aut Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies. canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry Federico Armando verfasserin aut Vanessa Maria Nippold verfasserin aut Karl Rohn verfasserin aut Philippe Plattet verfasserin aut Graham Brogden verfasserin aut Gisa Gerold verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 11, p 6156 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:11, p 6156 https://doi.org/10.3390/ijms23116156 kostenfrei https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 kostenfrei https://www.mdpi.com/1422-0067/23/11/6156 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 11, p 6156
allfields_unstemmed	10.3390/ijms23116156 doi (DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Katarzyna Marek verfasserin aut Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies. canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry Federico Armando verfasserin aut Vanessa Maria Nippold verfasserin aut Karl Rohn verfasserin aut Philippe Plattet verfasserin aut Graham Brogden verfasserin aut Gisa Gerold verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 11, p 6156 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:11, p 6156 https://doi.org/10.3390/ijms23116156 kostenfrei https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 kostenfrei https://www.mdpi.com/1422-0067/23/11/6156 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 11, p 6156
allfieldsGer	10.3390/ijms23116156 doi (DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Katarzyna Marek verfasserin aut Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies. canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry Federico Armando verfasserin aut Vanessa Maria Nippold verfasserin aut Karl Rohn verfasserin aut Philippe Plattet verfasserin aut Graham Brogden verfasserin aut Gisa Gerold verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 11, p 6156 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:11, p 6156 https://doi.org/10.3390/ijms23116156 kostenfrei https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 kostenfrei https://www.mdpi.com/1422-0067/23/11/6156 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 11, p 6156
allfieldsSound	10.3390/ijms23116156 doi (DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Katarzyna Marek verfasserin aut Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies. canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry Federico Armando verfasserin aut Vanessa Maria Nippold verfasserin aut Karl Rohn verfasserin aut Philippe Plattet verfasserin aut Graham Brogden verfasserin aut Gisa Gerold verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 11, p 6156 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:11, p 6156 https://doi.org/10.3390/ijms23116156 kostenfrei https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 kostenfrei https://www.mdpi.com/1422-0067/23/11/6156 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 11, p 6156
language	English
source	In International Journal of Molecular Sciences 23(2022), 11, p 6156 volume:23 year:2022 number:11, p 6156
sourceStr	In International Journal of Molecular Sciences 23(2022), 11, p 6156 volume:23 year:2022 number:11, p 6156
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Katarzyna Marek @@aut@@ Federico Armando @@aut@@ Vanessa Maria Nippold @@aut@@ Karl Rohn @@aut@@ Philippe Plattet @@aut@@ Graham Brogden @@aut@@ Gisa Gerold @@aut@@ Wolfgang Baumgärtner @@aut@@ Christina Puff @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ042843359
language_de	englisch
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callnumber-first	Q - Science
author	Katarzyna Marek
spellingShingle	Katarzyna Marek misc QH301-705.5 misc QD1-999 misc canine distemper virus misc canine histiocytic sarcoma misc CDV-Onderstepoort misc DH82 misc genetically engineered viruses misc GM-CSF misc Biology (General) misc Chemistry Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
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topic_title	QH301-705.5 QD1-999 Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor canine distemper virus canine histiocytic sarcoma CDV-Onderstepoort DH82 genetically engineered viruses GM-CSF
topic	misc QH301-705.5 misc QD1-999 misc canine distemper virus misc canine histiocytic sarcoma misc CDV-Onderstepoort misc DH82 misc genetically engineered viruses misc GM-CSF misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc canine distemper virus misc canine histiocytic sarcoma misc CDV-Onderstepoort misc DH82 misc genetically engineered viruses misc GM-CSF misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc canine distemper virus misc canine histiocytic sarcoma misc CDV-Onderstepoort misc DH82 misc genetically engineered viruses misc GM-CSF misc Biology (General) misc Chemistry
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title	Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
ctrlnum	(DE-627)DOAJ042843359 (DE-599)DOAJb956a9b68e6b406bb69a6dd1f467a0e1
title_full	Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
author_sort	Katarzyna Marek
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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lang_code	eng
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author_browse	Katarzyna Marek Federico Armando Vanessa Maria Nippold Karl Rohn Philippe Plattet Graham Brogden Gisa Gerold Wolfgang Baumgärtner Christina Puff
container_volume	23
class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Katarzyna Marek
doi_str_mv	10.3390/ijms23116156
author2-role	verfasserin
title_sort	persistent infection of a canine histiocytic sarcoma cell line with attenuated canine distemper virus expressing vasostatin or granulocyte-macrophage colony-stimulating factor
callnumber	QH301-705.5
title_auth	Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
abstract	Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies.
abstractGer	Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies.
abstract_unstemmed	Canine histiocytic sarcoma (HS) represents a neoplasia with poor prognosis. Due to the high metastatic rate of HS, there is urgency to improve treatment options and to prevent tumor metastases. Canine distemper virus (CDV) is a single-stranded negative-sense RNA (ssRNA (-)) virus with potentially oncolytic properties. Moreover, vasostatin and granulocyte-macrophage colony-stimulating factor (GM-CSF) are attractive molecules in cancer therapy research because of their anti-angiogenetic properties and potential modulation of the tumor microenvironment. In the present study, an <i<in vitro</i< characterization of two genetically engineered viruses based on the CDV strain Onderstepoort (CDV-Ond), CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< was performed. Canine histiocytic sarcoma cells (DH82 cells) were persistently infected with CDV-Ond, CDV-Ond<sup<neon</sup<, CDV-Ond<sup<neon-vasostatin</sup< and CDV-Ond<sup<neon-GM-CSF</sup< and characterized on a molecular and protein level regarding their vasostatin and GM-CSF production. Interestingly, DH82 cells persistently infected with CDV-Ond<sup<neon-vasostatin</sup< showed a significantly increased number of vasostatin mRNA transcripts. Similarly, DH82 cells persistently infected with CDV-Ond<sup<neon-GM-CSF</sup< displayed an increased number of GM-CSF mRNA transcripts mirrored on the protein level as confirmed by immunofluorescence and Western blot. In summary, modified CDV-Ond strains expressed GM-CSF and vasostatin, rendering them promising candidates for the improvement of oncolytic virotherapies, which should be further detailed in future <i<in vivo</i< studies.
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container_issue	11, p 6156
title_short	Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
url	https://doi.org/10.3390/ijms23116156 https://doaj.org/article/b956a9b68e6b406bb69a6dd1f467a0e1 https://www.mdpi.com/1422-0067/23/11/6156 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Federico Armando Vanessa Maria Nippold Karl Rohn Philippe Plattet Graham Brogden Gisa Gerold Wolfgang Baumgärtner Christina Puff
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up_date	2024-07-03T14:19:14.959Z
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Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor

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