Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans

l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases i...
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Gespeichert in:

Autor*in:	Li Liu [verfasserIn] Yue Chen [verfasserIn] Shiqin Yu [verfasserIn] Jian Chen [verfasserIn] Jingwen Zhou [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	D-sorbitol dehydrogenase Gluconobacter oxydans l-ascorbic acid Metabolic engineering

Übergeordnetes Werk:	In: Synthetic and Systems Biotechnology - KeAi Communications Co., Ltd., 2017, 7(2022), 2, Seite 730-737
Übergeordnetes Werk:	volume:7 ; year:2022 ; number:2 ; pages:730-737

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.synbio.2022.02.008

Katalog-ID:	DOAJ043252222

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allfields	10.1016/j.synbio.2022.02.008 doi (DE-627)DOAJ043252222 (DE-599)DOAJ21dd6860d88340f19812876ef75d26fd DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Li Liu verfasserin aut Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene knockout. Then, a total of 38 dehydrogenases were knocked out in G. oxydans WSH-003, and 23 dehydrogenase-deficient strains could increase l-sorbose production. G. oxydans-30, wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted, showed a significant reduction of a by-product with the extension of fermentation time. In addition, the highest conversion ratio of 99.60% was achieved in G. oxydans MD-16, in which 16 different types of dehydrogenases were inactivated consecutively. Finally, the gene vhb encoding hemoglobin was introduced into the strain. The titer of l-sorbose was 298.61 g/L in a 5-L bioreactor. The results showed that the systematic engineering of dehydrogenase could significantly enhance the production of l-sorbose. D-sorbitol dehydrogenase Gluconobacter oxydans l-ascorbic acid Metabolic engineering Biotechnology Biology (General) Yue Chen verfasserin aut Shiqin Yu verfasserin aut Jian Chen verfasserin aut Jingwen Zhou verfasserin aut In Synthetic and Systems Biotechnology KeAi Communications Co., Ltd., 2017 7(2022), 2, Seite 730-737 (DE-627)847423204 (DE-600)2846506-4 2405805X nnns volume:7 year:2022 number:2 pages:730-737 https://doi.org/10.1016/j.synbio.2022.02.008 kostenfrei https://doaj.org/article/21dd6860d88340f19812876ef75d26fd kostenfrei http://www.sciencedirect.com/science/article/pii/S2405805X22000242 kostenfrei https://doaj.org/toc/2405-805X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 2 730-737
spelling	10.1016/j.synbio.2022.02.008 doi (DE-627)DOAJ043252222 (DE-599)DOAJ21dd6860d88340f19812876ef75d26fd DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Li Liu verfasserin aut Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene knockout. Then, a total of 38 dehydrogenases were knocked out in G. oxydans WSH-003, and 23 dehydrogenase-deficient strains could increase l-sorbose production. G. oxydans-30, wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted, showed a significant reduction of a by-product with the extension of fermentation time. In addition, the highest conversion ratio of 99.60% was achieved in G. oxydans MD-16, in which 16 different types of dehydrogenases were inactivated consecutively. Finally, the gene vhb encoding hemoglobin was introduced into the strain. The titer of l-sorbose was 298.61 g/L in a 5-L bioreactor. The results showed that the systematic engineering of dehydrogenase could significantly enhance the production of l-sorbose. D-sorbitol dehydrogenase Gluconobacter oxydans l-ascorbic acid Metabolic engineering Biotechnology Biology (General) Yue Chen verfasserin aut Shiqin Yu verfasserin aut Jian Chen verfasserin aut Jingwen Zhou verfasserin aut In Synthetic and Systems Biotechnology KeAi Communications Co., Ltd., 2017 7(2022), 2, Seite 730-737 (DE-627)847423204 (DE-600)2846506-4 2405805X nnns volume:7 year:2022 number:2 pages:730-737 https://doi.org/10.1016/j.synbio.2022.02.008 kostenfrei https://doaj.org/article/21dd6860d88340f19812876ef75d26fd kostenfrei http://www.sciencedirect.com/science/article/pii/S2405805X22000242 kostenfrei https://doaj.org/toc/2405-805X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 2 730-737
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source	In Synthetic and Systems Biotechnology 7(2022), 2, Seite 730-737 volume:7 year:2022 number:2 pages:730-737
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authorswithroles_txt_mv	Li Liu @@aut@@ Yue Chen @@aut@@ Shiqin Yu @@aut@@ Jian Chen @@aut@@ Jingwen Zhou @@aut@@
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callnumber-first	T - Technology
author	Li Liu
spellingShingle	Li Liu misc TP248.13-248.65 misc QH301-705.5 misc D-sorbitol dehydrogenase misc Gluconobacter oxydans misc l-ascorbic acid misc Metabolic engineering misc Biotechnology misc Biology (General) Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans
authorStr	Li Liu
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topic_title	TP248.13-248.65 QH301-705.5 Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans D-sorbitol dehydrogenase Gluconobacter oxydans l-ascorbic acid Metabolic engineering
topic	misc TP248.13-248.65 misc QH301-705.5 misc D-sorbitol dehydrogenase misc Gluconobacter oxydans misc l-ascorbic acid misc Metabolic engineering misc Biotechnology misc Biology (General)
topic_unstemmed	misc TP248.13-248.65 misc QH301-705.5 misc D-sorbitol dehydrogenase misc Gluconobacter oxydans misc l-ascorbic acid misc Metabolic engineering misc Biotechnology misc Biology (General)
topic_browse	misc TP248.13-248.65 misc QH301-705.5 misc D-sorbitol dehydrogenase misc Gluconobacter oxydans misc l-ascorbic acid misc Metabolic engineering misc Biotechnology misc Biology (General)
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title	Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans
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title_full	Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans
author_sort	Li Liu
journal	Synthetic and Systems Biotechnology
journalStr	Synthetic and Systems Biotechnology
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author_browse	Li Liu Yue Chen Shiqin Yu Jian Chen Jingwen Zhou
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title_sort	enhanced production of l-sorbose by systematic engineering of dehydrogenases in gluconobacter oxydans
callnumber	TP248.13-248.65
title_auth	Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans
abstract	l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene knockout. Then, a total of 38 dehydrogenases were knocked out in G. oxydans WSH-003, and 23 dehydrogenase-deficient strains could increase l-sorbose production. G. oxydans-30, wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted, showed a significant reduction of a by-product with the extension of fermentation time. In addition, the highest conversion ratio of 99.60% was achieved in G. oxydans MD-16, in which 16 different types of dehydrogenases were inactivated consecutively. Finally, the gene vhb encoding hemoglobin was introduced into the strain. The titer of l-sorbose was 298.61 g/L in a 5-L bioreactor. The results showed that the systematic engineering of dehydrogenase could significantly enhance the production of l-sorbose.
abstractGer	l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene knockout. Then, a total of 38 dehydrogenases were knocked out in G. oxydans WSH-003, and 23 dehydrogenase-deficient strains could increase l-sorbose production. G. oxydans-30, wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted, showed a significant reduction of a by-product with the extension of fermentation time. In addition, the highest conversion ratio of 99.60% was achieved in G. oxydans MD-16, in which 16 different types of dehydrogenases were inactivated consecutively. Finally, the gene vhb encoding hemoglobin was introduced into the strain. The titer of l-sorbose was 298.61 g/L in a 5-L bioreactor. The results showed that the systematic engineering of dehydrogenase could significantly enhance the production of l-sorbose.
abstract_unstemmed	l-Sorbose is an essential intermediate for the industrial production of vitamin C (l-ascorbic acid). However, the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to l-sorbose. This study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene knockout. Then, a total of 38 dehydrogenases were knocked out in G. oxydans WSH-003, and 23 dehydrogenase-deficient strains could increase l-sorbose production. G. oxydans-30, wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted, showed a significant reduction of a by-product with the extension of fermentation time. In addition, the highest conversion ratio of 99.60% was achieved in G. oxydans MD-16, in which 16 different types of dehydrogenases were inactivated consecutively. Finally, the gene vhb encoding hemoglobin was introduced into the strain. The titer of l-sorbose was 298.61 g/L in a 5-L bioreactor. The results showed that the systematic engineering of dehydrogenase could significantly enhance the production of l-sorbose.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans
url	https://doi.org/10.1016/j.synbio.2022.02.008 https://doaj.org/article/21dd6860d88340f19812876ef75d26fd http://www.sciencedirect.com/science/article/pii/S2405805X22000242 https://doaj.org/toc/2405-805X
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author2	Yue Chen Shiqin Yu Jian Chen Jingwen Zhou
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doi_str	10.1016/j.synbio.2022.02.008
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up_date	2024-07-03T16:32:30.302Z
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Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans

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