<i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea

Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants respo...
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Autor*in:	Kexin Zhang [verfasserIn] Yu Duan [verfasserIn] Yu Cao [verfasserIn] Yiwen Chen [verfasserIn] Zhongwei Zou [verfasserIn] Fang Li [verfasserIn] Qiang Shen [verfasserIn] Xiaowei Yang [verfasserIn] Yuanchun Ma [verfasserIn] Wanping Fang [verfasserIn] Xujun Zhu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	tea γ-aminobutyric acid polyamine degradation

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 11(2022), 9, p 1356
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:9, p 1356

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods11091356

Katalog-ID:	DOAJ043518044

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spelling	10.3390/foods11091356 doi (DE-627)DOAJ043518044 (DE-599)DOAJcd73173009554cb486a3a38f0ea28ad9 DE-627 ger DE-627 rakwb eng TP1-1185 Kexin Zhang verfasserin aut <i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants responded to exogenous putrescine, resulting in a significant increase in GABA content, while the glutamate level did not change. At the same time, five <i<copper-containing amine oxidase</i< (<i<CuAO</i<) and eight <i<aminoaldehyde dehydrogenase</i< (<i<AMADH</i<) genes involved in the putrescine-derived GABA pathway were identified from the Tea Plant Information Archive. Expression analysis indicated that <i<CsCuAO1</i<, <i<CsCuAO3</i< as well as <i<CsAMADH1</i< were induced to play an important function in response to exogenous putrescine. Thus, the three genes were cloned and the catalytic efficiency of soluble recombinant proteins was determined. <i<CsCuAOs</i< and <i<CsAMADH1</i< exhibited indispensable functions in the GABA production from putrescine in vitro. Subcellular localization assays indicated that <i<CsAMADH1</i< was localized in plastid, while both <i<CsCuAO1</i< and <i<CsCuAO3</i< were localized in peroxisome. In addition, the synergistic effects of <i<CsCuAOs</i< and <i<CsAMADH1</i< were investigated by a transient co-expression system in <i<Nicotiana benthamiana</i<. Our data suggest that these three genes regulate the accumulation of GABA in tea by participating in the polyamine degradation pathway and improve the content of GABA in tea to a certain extent. The results will greatly contribute to the production of GABA tea. tea γ-aminobutyric acid polyamine degradation <i<copper-containing amine oxidase</i< <i<aminoaldehyde dehydrogenase</i< Chemical technology Yu Duan verfasserin aut Yu Cao verfasserin aut Yiwen Chen verfasserin aut Zhongwei Zou verfasserin aut Fang Li verfasserin aut Qiang Shen verfasserin aut Xiaowei Yang verfasserin aut Yuanchun Ma verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Foods MDPI AG, 2013 11(2022), 9, p 1356 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:9, p 1356 https://doi.org/10.3390/foods11091356 kostenfrei https://doaj.org/article/cd73173009554cb486a3a38f0ea28ad9 kostenfrei https://www.mdpi.com/2304-8158/11/9/1356 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1356
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allfieldsSound	10.3390/foods11091356 doi (DE-627)DOAJ043518044 (DE-599)DOAJcd73173009554cb486a3a38f0ea28ad9 DE-627 ger DE-627 rakwb eng TP1-1185 Kexin Zhang verfasserin aut <i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants responded to exogenous putrescine, resulting in a significant increase in GABA content, while the glutamate level did not change. At the same time, five <i<copper-containing amine oxidase</i< (<i<CuAO</i<) and eight <i<aminoaldehyde dehydrogenase</i< (<i<AMADH</i<) genes involved in the putrescine-derived GABA pathway were identified from the Tea Plant Information Archive. Expression analysis indicated that <i<CsCuAO1</i<, <i<CsCuAO3</i< as well as <i<CsAMADH1</i< were induced to play an important function in response to exogenous putrescine. Thus, the three genes were cloned and the catalytic efficiency of soluble recombinant proteins was determined. <i<CsCuAOs</i< and <i<CsAMADH1</i< exhibited indispensable functions in the GABA production from putrescine in vitro. Subcellular localization assays indicated that <i<CsAMADH1</i< was localized in plastid, while both <i<CsCuAO1</i< and <i<CsCuAO3</i< were localized in peroxisome. In addition, the synergistic effects of <i<CsCuAOs</i< and <i<CsAMADH1</i< were investigated by a transient co-expression system in <i<Nicotiana benthamiana</i<. Our data suggest that these three genes regulate the accumulation of GABA in tea by participating in the polyamine degradation pathway and improve the content of GABA in tea to a certain extent. The results will greatly contribute to the production of GABA tea. tea γ-aminobutyric acid polyamine degradation <i<copper-containing amine oxidase</i< <i<aminoaldehyde dehydrogenase</i< Chemical technology Yu Duan verfasserin aut Yu Cao verfasserin aut Yiwen Chen verfasserin aut Zhongwei Zou verfasserin aut Fang Li verfasserin aut Qiang Shen verfasserin aut Xiaowei Yang verfasserin aut Yuanchun Ma verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Foods MDPI AG, 2013 11(2022), 9, p 1356 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:9, p 1356 https://doi.org/10.3390/foods11091356 kostenfrei https://doaj.org/article/cd73173009554cb486a3a38f0ea28ad9 kostenfrei https://www.mdpi.com/2304-8158/11/9/1356 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1356
language	English
source	In Foods 11(2022), 9, p 1356 volume:11 year:2022 number:9, p 1356
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topic_facet	tea γ-aminobutyric acid polyamine degradation <i<copper-containing amine oxidase</i< <i<aminoaldehyde dehydrogenase</i< Chemical technology
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container_title	Foods
authorswithroles_txt_mv	Kexin Zhang @@aut@@ Yu Duan @@aut@@ Yu Cao @@aut@@ Yiwen Chen @@aut@@ Zhongwei Zou @@aut@@ Fang Li @@aut@@ Qiang Shen @@aut@@ Xiaowei Yang @@aut@@ Yuanchun Ma @@aut@@ Wanping Fang @@aut@@ Xujun Zhu @@aut@@
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topic_title	TP1-1185 <i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea tea γ-aminobutyric acid polyamine degradation <i<copper-containing amine oxidase</i< <i<aminoaldehyde dehydrogenase</i<
topic	misc TP1-1185 misc tea misc γ-aminobutyric acid misc polyamine degradation misc <i<copper-containing amine oxidase</i< misc <i<aminoaldehyde dehydrogenase</i< misc Chemical technology
topic_unstemmed	misc TP1-1185 misc tea misc γ-aminobutyric acid misc polyamine degradation misc <i<copper-containing amine oxidase</i< misc <i<aminoaldehyde dehydrogenase</i< misc Chemical technology
topic_browse	misc TP1-1185 misc tea misc γ-aminobutyric acid misc polyamine degradation misc <i<copper-containing amine oxidase</i< misc <i<aminoaldehyde dehydrogenase</i< misc Chemical technology
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hierarchy_parent_title	Foods
hierarchy_parent_id	737287632
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title	<i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea
ctrlnum	(DE-627)DOAJ043518044 (DE-599)DOAJcd73173009554cb486a3a38f0ea28ad9
title_full	<i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea
author_sort	Kexin Zhang
journal	Foods
journalStr	Foods
callnumber-first-code	T
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2022
contenttype_str_mv	txt
author_browse	Kexin Zhang Yu Duan Yu Cao Yiwen Chen Zhongwei Zou Fang Li Qiang Shen Xiaowei Yang Yuanchun Ma Wanping Fang Xujun Zhu
container_volume	11
class	TP1-1185
format_se	Elektronische Aufsätze
author-letter	Kexin Zhang
doi_str_mv	10.3390/foods11091356
author2-role	verfasserin
title_sort	<i<cscuaos</i< and <i<csamadh1</i< are required for putrescine-derived γ-aminobutyric acid accumulation in tea
callnumber	TP1-1185
title_auth	<i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea
abstract	Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants responded to exogenous putrescine, resulting in a significant increase in GABA content, while the glutamate level did not change. At the same time, five <i<copper-containing amine oxidase</i< (<i<CuAO</i<) and eight <i<aminoaldehyde dehydrogenase</i< (<i<AMADH</i<) genes involved in the putrescine-derived GABA pathway were identified from the Tea Plant Information Archive. Expression analysis indicated that <i<CsCuAO1</i<, <i<CsCuAO3</i< as well as <i<CsAMADH1</i< were induced to play an important function in response to exogenous putrescine. Thus, the three genes were cloned and the catalytic efficiency of soluble recombinant proteins was determined. <i<CsCuAOs</i< and <i<CsAMADH1</i< exhibited indispensable functions in the GABA production from putrescine in vitro. Subcellular localization assays indicated that <i<CsAMADH1</i< was localized in plastid, while both <i<CsCuAO1</i< and <i<CsCuAO3</i< were localized in peroxisome. In addition, the synergistic effects of <i<CsCuAOs</i< and <i<CsAMADH1</i< were investigated by a transient co-expression system in <i<Nicotiana benthamiana</i<. Our data suggest that these three genes regulate the accumulation of GABA in tea by participating in the polyamine degradation pathway and improve the content of GABA in tea to a certain extent. The results will greatly contribute to the production of GABA tea.
abstractGer	Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants responded to exogenous putrescine, resulting in a significant increase in GABA content, while the glutamate level did not change. At the same time, five <i<copper-containing amine oxidase</i< (<i<CuAO</i<) and eight <i<aminoaldehyde dehydrogenase</i< (<i<AMADH</i<) genes involved in the putrescine-derived GABA pathway were identified from the Tea Plant Information Archive. Expression analysis indicated that <i<CsCuAO1</i<, <i<CsCuAO3</i< as well as <i<CsAMADH1</i< were induced to play an important function in response to exogenous putrescine. Thus, the three genes were cloned and the catalytic efficiency of soluble recombinant proteins was determined. <i<CsCuAOs</i< and <i<CsAMADH1</i< exhibited indispensable functions in the GABA production from putrescine in vitro. Subcellular localization assays indicated that <i<CsAMADH1</i< was localized in plastid, while both <i<CsCuAO1</i< and <i<CsCuAO3</i< were localized in peroxisome. In addition, the synergistic effects of <i<CsCuAOs</i< and <i<CsAMADH1</i< were investigated by a transient co-expression system in <i<Nicotiana benthamiana</i<. Our data suggest that these three genes regulate the accumulation of GABA in tea by participating in the polyamine degradation pathway and improve the content of GABA in tea to a certain extent. The results will greatly contribute to the production of GABA tea.
abstract_unstemmed	Polyamines are a potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, studies on GABA enrichment in tea mostly focus on the GABA shunt, while the correlation between polyamine degradation and GABA formation in tea is largely unknown. In this study, tea plants responded to exogenous putrescine, resulting in a significant increase in GABA content, while the glutamate level did not change. At the same time, five <i<copper-containing amine oxidase</i< (<i<CuAO</i<) and eight <i<aminoaldehyde dehydrogenase</i< (<i<AMADH</i<) genes involved in the putrescine-derived GABA pathway were identified from the Tea Plant Information Archive. Expression analysis indicated that <i<CsCuAO1</i<, <i<CsCuAO3</i< as well as <i<CsAMADH1</i< were induced to play an important function in response to exogenous putrescine. Thus, the three genes were cloned and the catalytic efficiency of soluble recombinant proteins was determined. <i<CsCuAOs</i< and <i<CsAMADH1</i< exhibited indispensable functions in the GABA production from putrescine in vitro. Subcellular localization assays indicated that <i<CsAMADH1</i< was localized in plastid, while both <i<CsCuAO1</i< and <i<CsCuAO3</i< were localized in peroxisome. In addition, the synergistic effects of <i<CsCuAOs</i< and <i<CsAMADH1</i< were investigated by a transient co-expression system in <i<Nicotiana benthamiana</i<. Our data suggest that these three genes regulate the accumulation of GABA in tea by participating in the polyamine degradation pathway and improve the content of GABA in tea to a certain extent. The results will greatly contribute to the production of GABA tea.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	9, p 1356
title_short	<i<CsCuAOs</i< and <i<CsAMADH1</i< Are Required for Putrescine-Derived γ-Aminobutyric Acid Accumulation in Tea
url	https://doi.org/10.3390/foods11091356 https://doaj.org/article/cd73173009554cb486a3a38f0ea28ad9 https://www.mdpi.com/2304-8158/11/9/1356 https://doaj.org/toc/2304-8158
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author2	Yu Duan Yu Cao Yiwen Chen Zhongwei Zou Fang Li Qiang Shen Xiaowei Yang Yuanchun Ma Wanping Fang Xujun Zhu
author2Str	Yu Duan Yu Cao Yiwen Chen Zhongwei Zou Fang Li Qiang Shen Xiaowei Yang Yuanchun Ma Wanping Fang Xujun Zhu
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up_date	2024-07-03T18:00:11.872Z
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