Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks

In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and m...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Daniel Gilbert Weber [verfasserIn] Swaantje Casjens [verfasserIn] Peter Rozynek [verfasserIn] Martin Lehnert [verfasserIn] Sandra Zilch-Schöneweis [verfasserIn] Oleksandr Bryk [verfasserIn] Dirk Taeger [verfasserIn] Maria Gomolka [verfasserIn] Michaela Kreuzer [verfasserIn] Heinz Otten [verfasserIn] Beate Pesch [verfasserIn] Georg Johnen [verfasserIn] Thomas Brüning [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: Biomarker Insights - SAGE Publishing, 2007, 5(2010)
Übergeordnetes Werk:	volume:5 ; year:2010

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4137/BMI.S5522

Katalog-ID:	DOAJ043602975

Internformat


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912			\|a GBV_ILN_2111
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allfields	10.4137/BMI.S5522 doi (DE-627)DOAJ043602975 (DE-599)DOAJ84c7549e7f6c43189e89099e3c17f180 DE-627 ger DE-627 rakwb eng R5-920 Daniel Gilbert Weber verfasserin aut Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. Medicine (General) Swaantje Casjens verfasserin aut Peter Rozynek verfasserin aut Martin Lehnert verfasserin aut Sandra Zilch-Schöneweis verfasserin aut Oleksandr Bryk verfasserin aut Dirk Taeger verfasserin aut Maria Gomolka verfasserin aut Michaela Kreuzer verfasserin aut Heinz Otten verfasserin aut Beate Pesch verfasserin aut Georg Johnen verfasserin aut Thomas Brüning verfasserin aut In Biomarker Insights SAGE Publishing, 2007 5(2010) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:5 year:2010 https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 kostenfrei https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010
spelling	10.4137/BMI.S5522 doi (DE-627)DOAJ043602975 (DE-599)DOAJ84c7549e7f6c43189e89099e3c17f180 DE-627 ger DE-627 rakwb eng R5-920 Daniel Gilbert Weber verfasserin aut Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. Medicine (General) Swaantje Casjens verfasserin aut Peter Rozynek verfasserin aut Martin Lehnert verfasserin aut Sandra Zilch-Schöneweis verfasserin aut Oleksandr Bryk verfasserin aut Dirk Taeger verfasserin aut Maria Gomolka verfasserin aut Michaela Kreuzer verfasserin aut Heinz Otten verfasserin aut Beate Pesch verfasserin aut Georg Johnen verfasserin aut Thomas Brüning verfasserin aut In Biomarker Insights SAGE Publishing, 2007 5(2010) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:5 year:2010 https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 kostenfrei https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010
allfields_unstemmed	10.4137/BMI.S5522 doi (DE-627)DOAJ043602975 (DE-599)DOAJ84c7549e7f6c43189e89099e3c17f180 DE-627 ger DE-627 rakwb eng R5-920 Daniel Gilbert Weber verfasserin aut Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. Medicine (General) Swaantje Casjens verfasserin aut Peter Rozynek verfasserin aut Martin Lehnert verfasserin aut Sandra Zilch-Schöneweis verfasserin aut Oleksandr Bryk verfasserin aut Dirk Taeger verfasserin aut Maria Gomolka verfasserin aut Michaela Kreuzer verfasserin aut Heinz Otten verfasserin aut Beate Pesch verfasserin aut Georg Johnen verfasserin aut Thomas Brüning verfasserin aut In Biomarker Insights SAGE Publishing, 2007 5(2010) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:5 year:2010 https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 kostenfrei https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010
allfieldsGer	10.4137/BMI.S5522 doi (DE-627)DOAJ043602975 (DE-599)DOAJ84c7549e7f6c43189e89099e3c17f180 DE-627 ger DE-627 rakwb eng R5-920 Daniel Gilbert Weber verfasserin aut Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. Medicine (General) Swaantje Casjens verfasserin aut Peter Rozynek verfasserin aut Martin Lehnert verfasserin aut Sandra Zilch-Schöneweis verfasserin aut Oleksandr Bryk verfasserin aut Dirk Taeger verfasserin aut Maria Gomolka verfasserin aut Michaela Kreuzer verfasserin aut Heinz Otten verfasserin aut Beate Pesch verfasserin aut Georg Johnen verfasserin aut Thomas Brüning verfasserin aut In Biomarker Insights SAGE Publishing, 2007 5(2010) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:5 year:2010 https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 kostenfrei https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010
allfieldsSound	10.4137/BMI.S5522 doi (DE-627)DOAJ043602975 (DE-599)DOAJ84c7549e7f6c43189e89099e3c17f180 DE-627 ger DE-627 rakwb eng R5-920 Daniel Gilbert Weber verfasserin aut Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. Medicine (General) Swaantje Casjens verfasserin aut Peter Rozynek verfasserin aut Martin Lehnert verfasserin aut Sandra Zilch-Schöneweis verfasserin aut Oleksandr Bryk verfasserin aut Dirk Taeger verfasserin aut Maria Gomolka verfasserin aut Michaela Kreuzer verfasserin aut Heinz Otten verfasserin aut Beate Pesch verfasserin aut Georg Johnen verfasserin aut Thomas Brüning verfasserin aut In Biomarker Insights SAGE Publishing, 2007 5(2010) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:5 year:2010 https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 kostenfrei https://doi.org/10.4137/BMI.S5522 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2010
language	English
source	In Biomarker Insights 5(2010) volume:5 year:2010
sourceStr	In Biomarker Insights 5(2010) volume:5 year:2010
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container_title	Biomarker Insights
authorswithroles_txt_mv	Daniel Gilbert Weber @@aut@@ Swaantje Casjens @@aut@@ Peter Rozynek @@aut@@ Martin Lehnert @@aut@@ Sandra Zilch-Schöneweis @@aut@@ Oleksandr Bryk @@aut@@ Dirk Taeger @@aut@@ Maria Gomolka @@aut@@ Michaela Kreuzer @@aut@@ Heinz Otten @@aut@@ Beate Pesch @@aut@@ Georg Johnen @@aut@@ Thomas Brüning @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
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callnumber-first	R - Medicine
author	Daniel Gilbert Weber
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topic_title	R5-920 Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
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title	Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
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title_full	Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
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author_browse	Daniel Gilbert Weber Swaantje Casjens Peter Rozynek Martin Lehnert Sandra Zilch-Schöneweis Oleksandr Bryk Dirk Taeger Maria Gomolka Michaela Kreuzer Heinz Otten Beate Pesch Georg Johnen Thomas Brüning
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title_sort	assessment of mrna and microrna stabilization in peripheral human blood for multicenter studies and biobanks
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title_auth	Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
abstract	In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.
abstractGer	In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.
abstract_unstemmed	In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.
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title_short	Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
url	https://doi.org/10.4137/BMI.S5522 https://doaj.org/article/84c7549e7f6c43189e89099e3c17f180 https://doaj.org/toc/1177-2719
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Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks

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