Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim

<i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little informat...
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Autor*in:	Lu Tian [verfasserIn] Jingwei Shi [verfasserIn] Lin Yang [verfasserIn] Anzhi Wei [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	terpene expresssion

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 11(2022), 12, p 1746
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:12, p 1746

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods11121746

Katalog-ID:	DOAJ043722032

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520			\|a <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research.
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allfields	10.3390/foods11121746 doi (DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1 DE-627 ger DE-627 rakwb eng TP1-1185 Lu Tian verfasserin aut Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research. <i<Zanthoxylum bungeanum</i< Maxim. <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion Chemical technology Jingwei Shi verfasserin aut Lin Yang verfasserin aut Anzhi Wei verfasserin aut In Foods MDPI AG, 2013 11(2022), 12, p 1746 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:12, p 1746 https://doi.org/10.3390/foods11121746 kostenfrei https://doaj.org/article/c2cab3fb13ae43b19c8a2dfa59ceaec1 kostenfrei https://www.mdpi.com/2304-8158/11/12/1746 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1746
spelling	10.3390/foods11121746 doi (DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1 DE-627 ger DE-627 rakwb eng TP1-1185 Lu Tian verfasserin aut Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research. <i<Zanthoxylum bungeanum</i< Maxim. <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion Chemical technology Jingwei Shi verfasserin aut Lin Yang verfasserin aut Anzhi Wei verfasserin aut In Foods MDPI AG, 2013 11(2022), 12, p 1746 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:12, p 1746 https://doi.org/10.3390/foods11121746 kostenfrei https://doaj.org/article/c2cab3fb13ae43b19c8a2dfa59ceaec1 kostenfrei https://www.mdpi.com/2304-8158/11/12/1746 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1746
allfields_unstemmed	10.3390/foods11121746 doi (DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1 DE-627 ger DE-627 rakwb eng TP1-1185 Lu Tian verfasserin aut Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research. <i<Zanthoxylum bungeanum</i< Maxim. <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion Chemical technology Jingwei Shi verfasserin aut Lin Yang verfasserin aut Anzhi Wei verfasserin aut In Foods MDPI AG, 2013 11(2022), 12, p 1746 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:12, p 1746 https://doi.org/10.3390/foods11121746 kostenfrei https://doaj.org/article/c2cab3fb13ae43b19c8a2dfa59ceaec1 kostenfrei https://www.mdpi.com/2304-8158/11/12/1746 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1746
allfieldsGer	10.3390/foods11121746 doi (DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1 DE-627 ger DE-627 rakwb eng TP1-1185 Lu Tian verfasserin aut Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research. <i<Zanthoxylum bungeanum</i< Maxim. <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion Chemical technology Jingwei Shi verfasserin aut Lin Yang verfasserin aut Anzhi Wei verfasserin aut In Foods MDPI AG, 2013 11(2022), 12, p 1746 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:12, p 1746 https://doi.org/10.3390/foods11121746 kostenfrei https://doaj.org/article/c2cab3fb13ae43b19c8a2dfa59ceaec1 kostenfrei https://www.mdpi.com/2304-8158/11/12/1746 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1746
allfieldsSound	10.3390/foods11121746 doi (DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1 DE-627 ger DE-627 rakwb eng TP1-1185 Lu Tian verfasserin aut Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research. <i<Zanthoxylum bungeanum</i< Maxim. <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion Chemical technology Jingwei Shi verfasserin aut Lin Yang verfasserin aut Anzhi Wei verfasserin aut In Foods MDPI AG, 2013 11(2022), 12, p 1746 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:12, p 1746 https://doi.org/10.3390/foods11121746 kostenfrei https://doaj.org/article/c2cab3fb13ae43b19c8a2dfa59ceaec1 kostenfrei https://www.mdpi.com/2304-8158/11/12/1746 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1746
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topic_title	TP1-1185 Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim <i<Zanthoxylum bungeanum</i< Maxim <i<ZbDXS</i< <i<ZbFPS</i< terpene expresssion
topic	misc TP1-1185 misc <i<Zanthoxylum bungeanum</i< Maxim. misc <i<ZbDXS</i< misc <i<ZbFPS</i< misc terpene misc expresssion misc Chemical technology
topic_unstemmed	misc TP1-1185 misc <i<Zanthoxylum bungeanum</i< Maxim. misc <i<ZbDXS</i< misc <i<ZbFPS</i< misc terpene misc expresssion misc Chemical technology
topic_browse	misc TP1-1185 misc <i<Zanthoxylum bungeanum</i< Maxim. misc <i<ZbDXS</i< misc <i<ZbFPS</i< misc terpene misc expresssion misc Chemical technology
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title	Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim
ctrlnum	(DE-627)DOAJ043722032 (DE-599)DOAJc2cab3fb13ae43b19c8a2dfa59ceaec1
title_full	Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim
author_sort	Lu Tian
journal	Foods
journalStr	Foods
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author_browse	Lu Tian Jingwei Shi Lin Yang Anzhi Wei
container_volume	11
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author-letter	Lu Tian
doi_str_mv	10.3390/foods11121746
author2-role	verfasserin
title_sort	molecular cloning and functional analysis of <i<dxs</i< and <i<fps</i< genes from <i<zanthoxylum bungeanum</i< maxim
callnumber	TP1-1185
title_auth	Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim
abstract	<i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research.
abstractGer	<i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research.
abstract_unstemmed	<i<Zanthoxylum bungeanum</i< Maxim. (<i<Z. bungeanum</i<) has attracted attention for its rich aroma. The aroma of <i<Z</i<<i<. bungeanum</i< is mainly volatile terpenes synthesized by plant terpene metabolic pathways. However, there is little information on <i<Z. bungeanum</i< terpene metabolic gene. In this study, the coding sequence of <i<1-deoxy-D-xylulose-5-phosphate synthase</i< (<i<DXS</i<) and <i<farnesyl pyrophosphate synthase</i< (<i<FPS</i<) were cloned from <i<Z. bungeanum</i< cv. ‘Fengxiandahongpao.’ <i<ZbDXS</i< and <i<ZbFPS</i< genes from <i<Z. bungeanum</i< with CDS lengths of 2172 bp and 1029 bp, respectively. The bioinformatics results showed that <i<Z. bungeanum</i< was closely related to citrus, and it was deduced that <i<ZbFPS</i< were hydrophilic proteins without the transmembrane domain. Subcellular localization prediction indicated that <i<ZbDXS</i< was most likely to be located in chloroplasts, and <i<ZbFPS</i< was most likely to be in mitochondria. Meanwhile, the 3D protein structure showed that <i<ZbDXS</i< and <i<ZbFPS</i< were mainly composed of α-helices, which were folded into a single domain. In vitro enzyme activity experiments showed that purified proteins <i<ZbDXS</i< and <i<ZbFPS</i< had the functions of <i<DXS</i< enzyme and <i<FPS</i< enzyme. Transient expression of <i<ZbDXS</i< and <i<ZbFPS</i< in tobacco significantly increased tobacco’s terpene content. Moreover, <i<ZbDXS</i< and <i<ZbFPS</i< were expressed in different tissues of <i<Z. bungeanum</i<, and the relative expression of the two genes was the highest in fruits. Therefore, this suggests that <i<ZbDXS</i< and <i<ZbFPS</i< are positively related to terpene synthesis. This study could provide reference genes for improving <i<Z. bungeanum</i< breeding as well as for the Rutaceae research.
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container_issue	12, p 1746
title_short	Molecular Cloning and Functional Analysis of <i<DXS</i< and <i<FPS</i< Genes from <i<Zanthoxylum bungeanum</i< Maxim
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