Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy

Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum an...
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Autor*in:	Biao Huang [verfasserIn] Yi Zhang [verfasserIn] Liang Wang [verfasserIn] Wenwei Xu [verfasserIn] Jue Zhang [verfasserIn] Qiuhua Zhang [verfasserIn] Huiming Sheng [verfasserIn] Zhigang Hu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay

Übergeordnetes Werk:	In: Kidney & Blood Pressure Research - Karger Publishers, 2002, (2019), Seite 10
Übergeordnetes Werk:	year:2019 ; pages:10

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1159/000500456

Katalog-ID:	DOAJ043799620

Internformat


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520			\|a Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN.
650		4	\|a Phospholipase A2 receptor antibody
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650		4	\|a Idiopathic membranous nephropathy
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700	0		\|a Zhigang Hu \|e verfasserin \|4 aut
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author_variant	b h bh y z yz l w lw w x wx j z jz q z qz h s hs z h zh
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allfields	10.1159/000500456 doi (DE-627)DOAJ043799620 (DE-599)DOAJb38f9501a9384cf4b82519f0a1ff8358 DE-627 ger DE-627 rakwb eng RL1-803 RC666-701 RC870-923 Biao Huang verfasserin aut Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology Yi Zhang verfasserin aut Liang Wang verfasserin aut Wenwei Xu verfasserin aut Jue Zhang verfasserin aut Qiuhua Zhang verfasserin aut Huiming Sheng verfasserin aut Zhigang Hu verfasserin aut In Kidney & Blood Pressure Research Karger Publishers, 2002 (2019), Seite 10 (DE-627)300593767 (DE-600)1482922-8 14230143 nnns year:2019 pages:10 https://doi.org/10.1159/000500456 kostenfrei https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 kostenfrei https://www.karger.com/Article/FullText/500456 kostenfrei https://doaj.org/toc/1420-4096 Journal toc kostenfrei https://doaj.org/toc/1423-0143 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 10
spelling	10.1159/000500456 doi (DE-627)DOAJ043799620 (DE-599)DOAJb38f9501a9384cf4b82519f0a1ff8358 DE-627 ger DE-627 rakwb eng RL1-803 RC666-701 RC870-923 Biao Huang verfasserin aut Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology Yi Zhang verfasserin aut Liang Wang verfasserin aut Wenwei Xu verfasserin aut Jue Zhang verfasserin aut Qiuhua Zhang verfasserin aut Huiming Sheng verfasserin aut Zhigang Hu verfasserin aut In Kidney & Blood Pressure Research Karger Publishers, 2002 (2019), Seite 10 (DE-627)300593767 (DE-600)1482922-8 14230143 nnns year:2019 pages:10 https://doi.org/10.1159/000500456 kostenfrei https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 kostenfrei https://www.karger.com/Article/FullText/500456 kostenfrei https://doaj.org/toc/1420-4096 Journal toc kostenfrei https://doaj.org/toc/1423-0143 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 10
allfields_unstemmed	10.1159/000500456 doi (DE-627)DOAJ043799620 (DE-599)DOAJb38f9501a9384cf4b82519f0a1ff8358 DE-627 ger DE-627 rakwb eng RL1-803 RC666-701 RC870-923 Biao Huang verfasserin aut Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology Yi Zhang verfasserin aut Liang Wang verfasserin aut Wenwei Xu verfasserin aut Jue Zhang verfasserin aut Qiuhua Zhang verfasserin aut Huiming Sheng verfasserin aut Zhigang Hu verfasserin aut In Kidney & Blood Pressure Research Karger Publishers, 2002 (2019), Seite 10 (DE-627)300593767 (DE-600)1482922-8 14230143 nnns year:2019 pages:10 https://doi.org/10.1159/000500456 kostenfrei https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 kostenfrei https://www.karger.com/Article/FullText/500456 kostenfrei https://doaj.org/toc/1420-4096 Journal toc kostenfrei https://doaj.org/toc/1423-0143 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 10
allfieldsGer	10.1159/000500456 doi (DE-627)DOAJ043799620 (DE-599)DOAJb38f9501a9384cf4b82519f0a1ff8358 DE-627 ger DE-627 rakwb eng RL1-803 RC666-701 RC870-923 Biao Huang verfasserin aut Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology Yi Zhang verfasserin aut Liang Wang verfasserin aut Wenwei Xu verfasserin aut Jue Zhang verfasserin aut Qiuhua Zhang verfasserin aut Huiming Sheng verfasserin aut Zhigang Hu verfasserin aut In Kidney & Blood Pressure Research Karger Publishers, 2002 (2019), Seite 10 (DE-627)300593767 (DE-600)1482922-8 14230143 nnns year:2019 pages:10 https://doi.org/10.1159/000500456 kostenfrei https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 kostenfrei https://www.karger.com/Article/FullText/500456 kostenfrei https://doaj.org/toc/1420-4096 Journal toc kostenfrei https://doaj.org/toc/1423-0143 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 10
allfieldsSound	10.1159/000500456 doi (DE-627)DOAJ043799620 (DE-599)DOAJb38f9501a9384cf4b82519f0a1ff8358 DE-627 ger DE-627 rakwb eng RL1-803 RC666-701 RC870-923 Biao Huang verfasserin aut Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology Yi Zhang verfasserin aut Liang Wang verfasserin aut Wenwei Xu verfasserin aut Jue Zhang verfasserin aut Qiuhua Zhang verfasserin aut Huiming Sheng verfasserin aut Zhigang Hu verfasserin aut In Kidney & Blood Pressure Research Karger Publishers, 2002 (2019), Seite 10 (DE-627)300593767 (DE-600)1482922-8 14230143 nnns year:2019 pages:10 https://doi.org/10.1159/000500456 kostenfrei https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 kostenfrei https://www.karger.com/Article/FullText/500456 kostenfrei https://doaj.org/toc/1420-4096 Journal toc kostenfrei https://doaj.org/toc/1423-0143 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 10
language	English
source	In Kidney & Blood Pressure Research (2019), Seite 10 year:2019 pages:10
sourceStr	In Kidney & Blood Pressure Research (2019), Seite 10 year:2019 pages:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay Dermatology Diseases of the circulatory (Cardiovascular) system Diseases of the genitourinary system. Urology
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container_title	Kidney & Blood Pressure Research
authorswithroles_txt_mv	Biao Huang @@aut@@ Yi Zhang @@aut@@ Liang Wang @@aut@@ Wenwei Xu @@aut@@ Jue Zhang @@aut@@ Qiuhua Zhang @@aut@@ Huiming Sheng @@aut@@ Zhigang Hu @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
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spellingShingle	Biao Huang misc RL1-803 misc RC666-701 misc RC870-923 misc Phospholipase A2 receptor antibody misc IgG4 misc Idiopathic membranous nephropathy misc Membranous nephropathy misc Time-resolved fluoroimmunoassay misc Dermatology misc Diseases of the circulatory (Cardiovascular) system misc Diseases of the genitourinary system. Urology Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy
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topic_title	RL1-803 RC666-701 RC870-923 Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay
topic	misc RL1-803 misc RC666-701 misc RC870-923 misc Phospholipase A2 receptor antibody misc IgG4 misc Idiopathic membranous nephropathy misc Membranous nephropathy misc Time-resolved fluoroimmunoassay misc Dermatology misc Diseases of the circulatory (Cardiovascular) system misc Diseases of the genitourinary system. Urology
topic_unstemmed	misc RL1-803 misc RC666-701 misc RC870-923 misc Phospholipase A2 receptor antibody misc IgG4 misc Idiopathic membranous nephropathy misc Membranous nephropathy misc Time-resolved fluoroimmunoassay misc Dermatology misc Diseases of the circulatory (Cardiovascular) system misc Diseases of the genitourinary system. Urology
topic_browse	misc RL1-803 misc RC666-701 misc RC870-923 misc Phospholipase A2 receptor antibody misc IgG4 misc Idiopathic membranous nephropathy misc Membranous nephropathy misc Time-resolved fluoroimmunoassay misc Dermatology misc Diseases of the circulatory (Cardiovascular) system misc Diseases of the genitourinary system. Urology
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title	Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy
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title_full	Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy
author_sort	Biao Huang
journal	Kidney & Blood Pressure Research
journalStr	Kidney & Blood Pressure Research
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author_browse	Biao Huang Yi Zhang Liang Wang Wenwei Xu Jue Zhang Qiuhua Zhang Huiming Sheng Zhigang Hu
class	RL1-803 RC666-701 RC870-923
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doi_str_mv	10.1159/000500456
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title_sort	phospholipase a2 receptor antibody igg4 subclass improves sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy
callnumber	RL1-803
title_auth	Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy
abstract	Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN.
abstractGer	Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN.
abstract_unstemmed	Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69–2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 ± 15.15 ng/mL in 45 healthy volunteers, 31.08 ± 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 ± 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 ± 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN.
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title_short	Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy
url	https://doi.org/10.1159/000500456 https://doaj.org/article/b38f9501a9384cf4b82519f0a1ff8358 https://www.karger.com/Article/FullText/500456 https://doaj.org/toc/1420-4096 https://doaj.org/toc/1423-0143
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author2	Yi Zhang Liang Wang Wenwei Xu Jue Zhang Qiuhua Zhang Huiming Sheng Zhigang Hu
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