MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replic...
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Autor*in:	Yan Zhou [verfasserIn] Linlin Chen [verfasserIn] Jing Du [verfasserIn] Xiaoqing Hu [verfasserIn] Yuping Xie [verfasserIn] Jinyuan Wu [verfasserIn] Xiaochen Lin [verfasserIn] Na Yin [verfasserIn] Maosheng Sun [verfasserIn] Hongjun Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	rotavirus microrna 7 non-structural protein 5 gene replication anti-viral

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 12(2020), 2, p 209
Übergeordnetes Werk:	volume:12 ; year:2020 ; number:2, p 209

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v12020209

Katalog-ID:	DOAJ044047177

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520			\|a Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.
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allfields	10.3390/v12020209 doi (DE-627)DOAJ044047177 (DE-599)DOAJ3205225bdff540edb5c9eb4a7bf39856 DE-627 ger DE-627 rakwb eng QR1-502 Yan Zhou verfasserin aut MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology Linlin Chen verfasserin aut Jing Du verfasserin aut Xiaoqing Hu verfasserin aut Yuping Xie verfasserin aut Jinyuan Wu verfasserin aut Xiaochen Lin verfasserin aut Na Yin verfasserin aut Maosheng Sun verfasserin aut Hongjun Li verfasserin aut In Viruses MDPI AG, 2009 12(2020), 2, p 209 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:12 year:2020 number:2, p 209 https://doi.org/10.3390/v12020209 kostenfrei https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 kostenfrei https://www.mdpi.com/1999-4915/12/2/209 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 2, p 209
spelling	10.3390/v12020209 doi (DE-627)DOAJ044047177 (DE-599)DOAJ3205225bdff540edb5c9eb4a7bf39856 DE-627 ger DE-627 rakwb eng QR1-502 Yan Zhou verfasserin aut MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology Linlin Chen verfasserin aut Jing Du verfasserin aut Xiaoqing Hu verfasserin aut Yuping Xie verfasserin aut Jinyuan Wu verfasserin aut Xiaochen Lin verfasserin aut Na Yin verfasserin aut Maosheng Sun verfasserin aut Hongjun Li verfasserin aut In Viruses MDPI AG, 2009 12(2020), 2, p 209 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:12 year:2020 number:2, p 209 https://doi.org/10.3390/v12020209 kostenfrei https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 kostenfrei https://www.mdpi.com/1999-4915/12/2/209 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 2, p 209
allfields_unstemmed	10.3390/v12020209 doi (DE-627)DOAJ044047177 (DE-599)DOAJ3205225bdff540edb5c9eb4a7bf39856 DE-627 ger DE-627 rakwb eng QR1-502 Yan Zhou verfasserin aut MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology Linlin Chen verfasserin aut Jing Du verfasserin aut Xiaoqing Hu verfasserin aut Yuping Xie verfasserin aut Jinyuan Wu verfasserin aut Xiaochen Lin verfasserin aut Na Yin verfasserin aut Maosheng Sun verfasserin aut Hongjun Li verfasserin aut In Viruses MDPI AG, 2009 12(2020), 2, p 209 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:12 year:2020 number:2, p 209 https://doi.org/10.3390/v12020209 kostenfrei https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 kostenfrei https://www.mdpi.com/1999-4915/12/2/209 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 2, p 209
allfieldsGer	10.3390/v12020209 doi (DE-627)DOAJ044047177 (DE-599)DOAJ3205225bdff540edb5c9eb4a7bf39856 DE-627 ger DE-627 rakwb eng QR1-502 Yan Zhou verfasserin aut MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology Linlin Chen verfasserin aut Jing Du verfasserin aut Xiaoqing Hu verfasserin aut Yuping Xie verfasserin aut Jinyuan Wu verfasserin aut Xiaochen Lin verfasserin aut Na Yin verfasserin aut Maosheng Sun verfasserin aut Hongjun Li verfasserin aut In Viruses MDPI AG, 2009 12(2020), 2, p 209 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:12 year:2020 number:2, p 209 https://doi.org/10.3390/v12020209 kostenfrei https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 kostenfrei https://www.mdpi.com/1999-4915/12/2/209 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 2, p 209
allfieldsSound	10.3390/v12020209 doi (DE-627)DOAJ044047177 (DE-599)DOAJ3205225bdff540edb5c9eb4a7bf39856 DE-627 ger DE-627 rakwb eng QR1-502 Yan Zhou verfasserin aut MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology Linlin Chen verfasserin aut Jing Du verfasserin aut Xiaoqing Hu verfasserin aut Yuping Xie verfasserin aut Jinyuan Wu verfasserin aut Xiaochen Lin verfasserin aut Na Yin verfasserin aut Maosheng Sun verfasserin aut Hongjun Li verfasserin aut In Viruses MDPI AG, 2009 12(2020), 2, p 209 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:12 year:2020 number:2, p 209 https://doi.org/10.3390/v12020209 kostenfrei https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 kostenfrei https://www.mdpi.com/1999-4915/12/2/209 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 2, p 209
language	English
source	In Viruses 12(2020), 2, p 209 volume:12 year:2020 number:2, p 209
sourceStr	In Viruses 12(2020), 2, p 209 volume:12 year:2020 number:2, p 209
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institution	findex.gbv.de
topic_facet	rotavirus microrna 7 non-structural protein 5 gene replication anti-viral Microbiology
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container_title	Viruses
authorswithroles_txt_mv	Yan Zhou @@aut@@ Linlin Chen @@aut@@ Jing Du @@aut@@ Xiaoqing Hu @@aut@@ Yuping Xie @@aut@@ Jinyuan Wu @@aut@@ Xiaochen Lin @@aut@@ Na Yin @@aut@@ Maosheng Sun @@aut@@ Hongjun Li @@aut@@
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callnumber-first	Q - Science
author	Yan Zhou
spellingShingle	Yan Zhou misc QR1-502 misc rotavirus misc microrna 7 misc non-structural protein 5 misc gene replication misc anti-viral misc Microbiology MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
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topic_title	QR1-502 MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro rotavirus microrna 7 non-structural protein 5 gene replication anti-viral
topic	misc QR1-502 misc rotavirus misc microrna 7 misc non-structural protein 5 misc gene replication misc anti-viral misc Microbiology
topic_unstemmed	misc QR1-502 misc rotavirus misc microrna 7 misc non-structural protein 5 misc gene replication misc anti-viral misc Microbiology
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title	MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
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title_full	MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
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title_sort	microrna-7 inhibits rotavirus replication by targeting viral nsp5 in vivo and in vitro
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title_auth	MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
abstract	Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.
abstractGer	Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.
abstract_unstemmed	Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.
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title_short	MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
url	https://doi.org/10.3390/v12020209 https://doaj.org/article/3205225bdff540edb5c9eb4a7bf39856 https://www.mdpi.com/1999-4915/12/2/209 https://doaj.org/toc/1999-4915
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MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

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